JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (1): 1-4.doi: 10.6040/j.issn.1671-7554.0.2013.383

• Articles •     Next Articles

Effect of CD40siRNA on CD4+CD25+ regulatory T cells in rats with experimental autoimmune myocarditis

WANG Jie-zhong1, HAN Bo1, GAO Ling2, YANG Wen-wei1, LIU Feng-qin1, Lv-Jian-li1, Zhao Li-jian1   

  1. 1. Department of Pediatric Cardiology; 2. Central Laboratory, Shandong Provincial Hospital Affiliated to
    Shandong University, Jinan 250021, China
  • Received:2013-06-14 Online:2014-01-10 Published:2014-01-10

PDF (PC)

427

Abstract:

 Objective  To investigate the therapeutic effect of CD40 siRNA on viral myocarditis and its effect on CD4+CD25+ regulatory T cells (Treg). Methods  A total of 40 male Lewis rats were randomly divided into four groups: normal control group, experimental autoimmune myocarditis (EAM) model group, CD40 siRNA treatment group and siRNA treatment group, with 10 rats in each group. On the 1st and 8th day, 0.2mL PBS buffer was injected subcutaneously into the two hind footpads of rats in the control group, while 0.2mL porcine cardiac myosin was injected subcutaneously into the two hind footpads of rats in the other three groups. On the 8th day, 25μL CD40siRNA lentiviral expression vector was injected into the tail vein of rats in the CD40siRNA treatment group, and 25μL siRNA lentiviral expression vector was injected into the tail vein of rats in the siRNA group. After all rats were sacrificed on the 21st day, myocardial pathological changes were observed with optical microscope, and CD4+CD25+Treg expression in rats’ spleen was determined with flow cytometry. Results  The myocardial histopathology integral of the CD40siRNA treatment group (11.70 ± 2.95) was significantly lower than that of the EAM model group (17.00 ± 1.76) (P<0.05). The CD4+CD25+Treg expression was significantly higher in the CD40siRNA treatment group (40.70±4.00) than in the EAM model group (12.20±1.10) (P<0.05). Conclusion  CD40siRNA can relieve myocarditis in EAM rats. The mechanism may be related to the up-regulation of CD4+CD25+Treg expression.

Key words: Myocarditis, EAM rats, CD40siRNA, Regulatory T cells

[1] LI Xue, LI Dong, SHI Qing, ZHOU Panpan, JU Xiuli. Expression and role of Helios gene in regulatory T cells of pediatric precursor B-cell acute lymphoblastic leukemia [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(4): 76-81.
[2] LIU Mengmeng, ZHAO Cuifen, KONG Qingyu, CAI Zhifeng, XIA Wei. miR-1/133 participates in the expression of ion channel genes of myocardial cells in myocarditis mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(8): 6-11.
[3] CEN Zhihong, GUO Yujie, WU Weifeng, LI Liping, ZHOU Qiuxi. Changes of regulatory B cells in mice with viral myocarditis induced by Coxsackie virus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 31-35.
[4] ZHANG Rongjun, HAN Bo, GAO Ling, ZHU Mei, DING Guoyu, LIANG Yan. Effects of CD40 knockdown by siRNA on rats with experimental autoimmune myocarditis and IL-22 expression [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 36-40.
[5] LU Guan-yan1, CUI Bin2, LIU Zhong-liang3, LI Yu-tang4, LIU Xue-fei3, MA Xiao-jing1, ZHU Gui-yue1, YUAN Hai-tao1. Valsartan alters the balance of Th17 cells to regulatory T cells in chronic viral  myocarditis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(2): 1-.
[6] LI Qiang1,2, CAI Da-xing1, CHEN Guang3, LIU Ji-feng4, LI Chun-yang1. Detection and significance of CD4+CD25+ Foxp3+ regulatory T cells in peripheral blood of patients with vitiligo [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(1): 133-.
[7] GUO Peng1, LIU Chuan-fang2, ZHAO Wen3. Effects of Wharton′s jelly-derived mesenchymal stem cells from the human umbilical cord on regulatory T cells and the Foxp3 genein severe aplastic anemia patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(9): 71-.
[8] SUN Shijing, HAN Bo, CHEN Yongfen， LU Kang, XI Junfang， SUN Jin. Expression and significance of Galectin-9 in murine with viral myocarditis  [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(8): 21-.
[9] CAO Xia, WANG Lian-lian, LUO Xi. Expression of regulatory T cells and helper T lymphocytes in the peripheral  blood of pregnancy induced hypertension women [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(6): 137-139.
[10] YU Yuan1, LI Yan1, RONG Fengnian2, LIANG Jing1, LIU Xiaolin1, WANG Fuli1. Therapy effect of auto-CIK on regulatory T cells in ovarian neoplasms patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(5): 101-104.
[11] LIU Fengqin1, WANG Yulin1, JIN Youpeng1, ZHU Xiaoxin2, WANG Qingqing1, SONG Jia1. Antiviral effect and inhibition of cardiomyocyte apoptosis of the Qishaowuweizi compound [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(2): 33-37.
[12] SUN Lei1，WU Jing-jing2，YI Shou-nan2，CHEN Li1. Isolation and amplification of human CD4+CD25+ regulatory T cells in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(12): 1-.
[13] FANG Yanni, GUO Chunyan, WANG Yi. Interventional effects of Captopril and Oxymatrine on anti-cardiac myosin antibody expression in mice with chronic viral myocarditis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(1): 25-29.
[14] WANG Hong-Wei, ZHANG Ying-, CHEN Fu-Qin. Expression of FOXP3, GITR and CD25 genes in peripheral blood of patients with Graves′ disease [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(12): 1-5.
[15] LIU Zhen-hu,HAN Bo,GUO Chun-yan,CHANG Hong,ZHANG Jie,HAN Xiu-zhen. Effect of the CD4+ T lymphocyte in the development of viral myocarditis in mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(5): 462-465.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd