Journal of Shandong University (Health Sciences) ›› 2024, Vol. 62 ›› Issue (10): 87-97.doi: 10.6040/j.issn.1671-7554.0.2024.0652

• Preclinical Medicine • Previous Articles    

Relationship between fusobacterium nucleatum mediated autophagy and cell refractive index based on optical surface wave

YU Li1, WANG Yijia2, YANG Yong3, LIU Xuehuan2, WAN Xuehua4, BAO Cuiping2, MIAO Beiliang5, LI Siqi1, LI Jing1, LIU Jun6   

  1. 1. School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;
    2. Translational Medicine Research Institute of Nankai University Peoples Hospital, Tianjin 300121, China;
    3. Research Center for Frontier Fundamental Studies, Zhejiang Laboratory, Hangzhou 311100, Zhejiang, China;
    4. Teda Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, China;
    5. Department of Nephrology and Endocrinology, Wangjing Hospital, Beijing 100102, China;
    6. Department of Radiology, Tianjin Fourth Center Hospital, Tianjin 300142, China
  • Published:2024-10-12

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Abstract: Objective To explore the relationship between autophagy levels mediated by Fusobacterium nucleatum(Fn)and changes in cellular refractive index using optical surface wave sensing technology and to preliminarily investigates the potential of optical surface wave technology for detecting autophagy levels in cancer cells. Methods CCK-8 was used to assess the impact of Fn on the proliferation activity of colorectal cancer cells. The effects of Fn on autophagy levels were examined using both Western blotting and dual-labeling fluorescence microscopy techniques. Additionally, optical surface wave technology was employed to monitor changes in cellular refractive index under different Fn infection states, and to observe their correlation with autophagy levels. Results When multiplicity of infection was 100 and the infection time was 4 hours, the protein expression levels of Beclin-1 and LC3 were increased(P<0.05), while the expression level of P62 was decreased(P<0.01), indicating elevated autophagy levels. Concurrently, optical surface wave showed that the refractive indices of HCT116 and HT29 cells also increased with the elevation of autophagy levels(P<0.01). Further investigation revealed that changes in cellular refractive index were primarily due to variations in autophagosome levels within the cells, and were not influenced by factors such as cell proliferation or extracellular substances. Conclusion The autophagy levels mediated by Fn correlate with cellular refractive index, indicating that optical surface wave sensing technology can dynamically reflect changes in Fn-mediated autophagy levels. This suggests that optical surface wave technology could serve as a rapid, intuitive, and non-invasive method to assess autophagy levels without affecting cellular viability.

Key words: Fusobacterium nucleatum, Colorectal cancer, Autophagy, Optical surface wave technique, Cell refractive index

CLC Number: 

  • Q28
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