Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (10): 64-71.doi: 10.6040/j.issn.1671-7554.0.2017.795

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Umbilical cord blood NK cells improved liver injury in sub-acute aging rats

LAN Jing1, LI Dong2, SHI Qing2, LIU Zilin1, ZHOU Panpan1, JU Xiuli1,2   

  1. 1. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Stem Cell and Regenerative Medicine Research Center of Shandong University, Jinan 250012, Shandong, China
  • Received:2017-08-18 Published:2022-09-27

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Abstract: Objective To investigate the feasibility and molecular mechanism of umbilical cord blood NK cells in improving liver injury in sub-acute aging rat models. Methods NK cells in cord blood(CB)were amplified from fresh CB mononuclear cells using genetically engineered K562 cells. Male Wistar rats aged 8-10 weeks were randomly divided into four groups: Normal group, Model group, Mod-Con group and Mod-NK group. Body weight and liver index, superoxide dismutase(SOD)and malondialdehyde(MDA)levels in serum and liver, and genes related to apoptosis were tested one week after the experiment. The morphology of liver sections was observed. Results Sub-acute aging rat models could be constructed successfully by subcutaneous injection of D-galactose. The transfusion of high-purity and high-concentration NK cells could significantly promote the recovery of liver injury and body weight(P<0.01), decrease the liver index(P<0.05), enhance the SOD activity(P<0.01), and reduce the level of MDA in serum and liver tissues(P<0.01). The liver sections showed that hepatic edema relieved. Masson staining indicted that fibrosis of liver decreased. Expression of apoptosis promoting genes decreased(P<0.01), while anti-apoptotic gene and fibroblast growth factor increased(P<0.01). Conclusion Transfusion of umbilical cord blood NK cells can effectively improve the liver injury in sub-acute aging rats.

Key words: Natural killer cells, Sub-acute aging, Anti-aging, Liver injury

CLC Number: 

  • R332
[1] 史进方, 顾国浩, 张学光. 免疫衰老及其免疫学预警指标[J]. 现代免疫学, 2005, 25(4): 347-349.
[2] Pita-Lopez ML, Pera A, Solana R. Adaptive memory of human NK-like CD8+ T-Cells to aging, and viral and tumor antigens[J]. Front Immunol, 2016, 7: 616. doi: 10.3389/fimmu.2016.00616.
[3] Pereira BI, Akbar AN. Convergence of innate and adaptive immunity during human aging [J]. Front Immunol, 2016, 7: 445. eCollection 2016.
[4] Lutz CT, Quinn LS. Sarcopenia, obesity, and natural killer cell immune senescence in aging: altered cytokine levels as a common mechanism[J]. Aging, 2012, 4(8): 535-546.
[5] Vivier E, Raulet DH, Moretta A, et al. Innate or adaptive immunity? The example of natural killer cells[J]. Science, 2011, 331(6013): 44-49.
[6] 丁琳. 自然杀伤细胞在病毒性肝衰竭中的作用及趋化机制研究[D]. 武汉: 华中科技大学, 2013.
[7] 魏海荣. NK细胞功能异常导致HBV慢性免疫逃逸和急性肝再生抑制的机制研究[D]. 合肥: 中国科学技术大学, 2010.
[8] 杨银莉, 张建. 慢性HBV感染引起NK细胞功能受损的分子机制研究[D]. 济南: 山东大学, 2016.
[9] Song X, Bao M, Li D, et al. Advanced glycation in D-galactose induced mouse aging model [J]. Mech Ageing Dev, 1999, 108(3): 239-251.
[10] Fujisaki H, Kakuda H, Shimasaki N, et al. Expansion of highly cytotoxic human natural killer cells for cancer cell therapy[J]. Cancer Res, 2009, 69(9): 4010-4017.
[11] Strauss-Albee DM, Horowitz A, Parham P, et al. Coordinated regulation of NK receptor expression in the maturing human immune system[J]. J Immunol, 2014, 193(10): 4871-4879.
[12] Davis SJ, Ikemixu S, Evans EJ, et al. The nature of molecular recognition by T cells [J]. Nat Immunol, 2003, 4(3): 217-224.
[13] Mu X, Zhang Y, Li J, et al. Angelica sinensis polysaccharide prevents hematopoietic stem cells senescence in D-Galactose-induced aging mouse model[J]. Stem Cells Int, 2017, 2017: 3508907. doi: 10.1155/2017/3508907.
[14] Shah NN, Baird K, Delbrook CP, et al. Acute GVHD in patients receiving IL-15/4-1BBL activated NK cells following T-cell-depleted stem cell transplantation[J]. Blood, 2015, 125(5): 784-792.
[15] 王少康, 孙桂菊, 张建新, 等. 亚急性衰老动物模型的建立与评价[J]. 东南大学学报(医学版), 2002, 21(3): 217-220. WANG Shaokang, SUN Guiju, ZHANG Jianxin, et al. Development and estimation of an animal sub-acute aging model induced by D-galactose [J]. Journal of Southeast University(Medical Science Edition), 2002, 21(3): 217-220.
[16] Chen C, Huang L, Nong Z, et al. Hyperharic oxygen prevents cognitive impairments in mice induced by D-galactose by improving cholinergic and anti-apoptotic functions [J]. Neurochem Res, 2017, 42(4): 1240-1253.
[17] Khan MZ, Atlas N, Nawaz W. Neuroprotective effects of Caralluma tuberculata on ameliorating cognitive impairment in a d-galactose-induced mouse model [J]. Biomed Pharmacother, 2016, 84: 387-394. doi: 10.1016/j.biopha.2016.09.055.
[18] Aydın AF, Çoban J, Dogan-Ekici I, et al. Carnosine and taurine treatments diminished brain oxidative stress and apoptosis in D-galactose aging model [J]. Metab Brain Dis, 2016, 31(2): 337-345.
[19] 董雅洁, 高维娟. Bcl-2、Bax、Caspase-3在细胞凋亡中的作用及其关系[J]. 中国老年学杂志, 2012, 32(21): 4828-4830.
[20] Bueno V, Sant'Anna OA, Lord JM. Ageing and myeloid-derived suppressor cells: possible involvement in immunosenescence and age-related disease[J]. Age, 2014, 36(6): 9729.
[21] Coppé JP, Patil CK, Rodier F, et al. Senescence-associated secretory phenotypes reveal cell-nonautonomous functions of oncogenic RAS and the p53 tumor suppressor [J]. PLoS Biol, 2008, 6(12): 2853-2868.
[22] Sagiv A, Krizhanovsky V. Immunosurveillance of senescent cells: the bright side of the senescence program [J]. Biogerontology, 2013, 14(6): 617-628.
[23] Krizhanovsky V, Yon M, Dickins RA, et al. Senescence of activated stellate cells limits liver fibrosis [J]. Cell, 2008, 134(4): 657-667.
[24] Xue W, Zender L, Miething C, et al. Senescence and tumour clearance is triggered by p53 restoration in murine liver carcinomas[J]. Nature, 2007, 445(7128): 656-660.
[25] Dalle JH, Meneses J, Wagner E, et al. Characterization of cord blood natural killer cells: implications for transplantation and neonatal infections [J]. Pediatr Res, 2005, 57(5pt1): 649-655.
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