JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (8): 6-11.doi: 10.6040/j.issn.1671-7554.0.2015.1171

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miR-1/133 participates in the expression of ion channel genes of myocardial cells in myocarditis mice

LIU Mengmeng, ZHAO Cuifen, KONG Qingyu, CAI Zhifeng, XIA Wei   

  • Received:2015-11-26 Online:2016-08-10 Published:2016-08-10

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Abstract: Objective To explore the involvement of miR-1/133 in regulating the expression of potassium and calcium ion channel in mouse with acute viral myocarditis. Methods After the mice models of acute viral myocarditis were established, they were divided into 4 groups: control group, viral myocarditis group, myocarditis+miR-1/133 mimics group, and myocarditis+miR-1/133 NC group, with 10 mice in each group. The myocardial pathological changes were observed with HE staining. The expressions of miR-1, miR-133, Kcnd2, Irx5, Kcnj2, and α1c in myocardium were detected with qRT-PCR. The expressions of Kv4.2, Kir2.1, and Cav1.2 in myocardium were detected with Western blotting. Results HE staining showed that, in the control group, myocardial cells arranged orderly, and no inflammatory cells infiltrated myocardium matrix; in the myocarditis group and myocarditis+miR-1/133 NC group, myocardial cells swelled and arranged disorderly, and inflammatory cells infiltrated myocardium matrix; in the myocarditis+miR-1/133 mimics group, myocardial cells arranged orderly without cell edema, and few inflammatory cells infiltrated myocardium matrix. Compared with the control group, in the myocarditis group and myocarditis+miR-1/133 NC group, the expressions of miR-1, miR-133, Kcnd2, Kcnj2, Kv4.2 and Kir2.1 in the myocardium significantly decreased(P<0.01), while the expressions of Irx5, α1c and Cav1.2 increased(P<0.01). Compared with the myocarditis 山 东 大 学 学 报 (医 学 版)54卷8期 -刘蒙蒙,等.miR-1/133对病毒性心肌炎小鼠心肌细胞离子通道表达的影响 \=-group and myocarditis+miR-1/133 NC group, in the myocarditis+miR-1/133 mimics group, the expressions of miR-1, miR-133, Kcnd2, Kcnj2, Kv4.2 and Kir2.1 in the myocardium were upregulated(P<0.05), while the expressions of Irx5, α1c and Cav1.2 were downregulated(P<0.01). Conclusion miR-1/133 is involved in the regulation of calcium and potassium ion channel gene expression in mice with acute viral myocarditis.

Key words: Potassium channel, miR-1, miR-133, Viral myocarditis, Calcium channel, Gene expression

CLC Number: 

  • R725.4
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