贵州地区临床酵母菌种类组成和分布

doi:10.6040/j.issn.1671-7554.0.2014.141

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (8): 94-97.doi: 10.6040/j.issn.1671-7554.0.2014.141

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Composition and distribution of clinical yeasts in Guizhou region, China

WANG Meizhu¹, CAO Yu², ZHAO Liang¹, XU Xiaoshan³, ZHANG Peifang³, LV Qian¹, BAI Fengyan⁴, KANG Yingqian¹

1. Department of Microbiology, Guiyang Medical College, Guiyang 550004, Guizhou, China;
2. Department of Dermatology and Venereal Disease, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou, China;
3. Department of Obstetrics and Gynecology, Baiyun People's Hospital, Guiyang 550014, Guizhou, China;
4. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

Received:2014-03-14 Revised:2014-06-18 Online:2014-08-10 Published:2014-08-10

Abstract

Abstract: Objectives To survey the species composition and distribution of clinical yeasts in Guizhou region. Methods Morphological observation, ITS1-SSCP patterning and sequencing were performed to identify 248 yeast strains from different clinical origins in Guizhou region, China. Results The identification result suggested that the isolated strains came from 2 genus and 10 species, in which the dominant genus and species were Candida and C.albicans, respectively. The molecular identification and CHROMagar testing coincidence rate was 91.53%. The distribution of different sources of clinical yeasts was significantly different in Guizhou region. Conclusion The combination of ITS1-SSCP and sequencing analyses can be accuracy and reliable when identifying the clinical yeasts with similar phenotypes. Although the most popular clinical yeast species was still C.albicans in local area, the increasing trend of non-albicans Candida species was noticed in this study, especially C.glabrata strains isolated from vulvovaginal andidiasis (VVC) patients.

Key words: Yeast, Strain identification, Guizhou, Single-strand conformation polymorphism

CLC Number:

R379

WANG Meizhu, CAO Yu, ZHAO Liang, XU Xiaoshan, ZHANG Peifang, LV Qian, BAI Fengyan, KANG Yingqian. Composition and distribution of clinical yeasts in Guizhou region, China[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(8): 94-97.

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