JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (11): 16-20.doi: 10.6040/j.issn.1671-7554.0.2014.039

Previous Articles     Next Articles

Safety of reconstituted bone xenografts on neuronal cells

WANG Xianmei, LI Na, SUN Xiaoxia, LIU Chenghu, HOU Li   

  1. CFDA-Jinan Quality Supervision and Inspection Center for Medical Devices; Shandong Provincial Key Laboratory of Biological Evaluation of Medical Device, Jinan 250101, Shandong, China
  • Received:2014-01-11 Published:2014-11-10

Abstract: Objective To evaluate the clinical safety of reconstituted bone xenografts on the nervous system by studying the influence on neuronal cell viability and apoptosis in Wistar rats. Methods Hippocampal and cortical neurons cells were cultured in the reconstituted bone xenograft extracts (preparation of liquid extracts was in accordance with ISO 10993.12-2002). The neuronal cells were divided into two groups: the reconstituted bone xenograft group, and the negative control group. Tests were conducted to study the viability and apoptosis of neuronal cells in different contact time and culture time with MTT incorporation assay and flow cytometry. Results There were no significant differences in neuronal cell viability and apoptosis between the two groups (P >0.05). Conclusion The reconstituted bone xenografts have no obvious influence on neuronal cell viability and apoptosis in Wistar rats. The result of this study may provide an experimental and theoretical basis for the safe clinical application of reconstituted bone xenografts.

Key words: Cell viability, Neuronal cells, Reconstituted bone xenograft, Cell apoptosis

CLC Number: 

  • R338.1
[1] Bauer T W. Bone graft substitutes[J]. Skeletal Radiol, 2007, 36(12): 1105-1107.
[2] Urabe K, Itoman M, Toyama Y, et al. Current trends in bone grafting and the issue of banked bone allografts based on the fourth nationwide survey of bone grafting status from 2000 to 2004[J]. J Orthop Sci, 2007, 12(6): 520-525.
[3] 邱贵兴, 孙世荃. 同种异体骨植入材料的临床应用[J]. 中华骨科杂志, 2004, 24(10): 635-637. QIU Guixing, SUN Shiquan. Clinical application of allogeneic bone implant materials[J]. Chinese Journal of Orthopaedics, 2004, 24(10): 635-637.
[4] 王峰. 人工重组合异体骨的临床应用[J]. 安徽中医学院学报, 2005, 24(5): 49-50. WANG Feng. Clinical application of artificial recombinant bone xenograft[J]. Journal of Anhui Traditional Chinese Medical College, 2005, 24(5): 49-50.
[5] 于忠英, 袁志, 张伟, 等. 重组合异种骨治疗股骨非感染性骨不连的长期疗效观察[J]. 现代生物医学进展, 2012, 12(7): 1303-1305. YU Zhongying, YUAN Zhi, ZHANG Wei, et al. Long-term efficacy observation of recombinant bone xenograft in treatment of femoral non-infected nonunion[J]. Progress in Modern Biomedicine, 2012, 12(7): 1303-1305.
[6] Zhao L, Sheng A L, Huang S H, et al. Mechanism underlying activity-dependent insertion of TrkB into the neuronal surface[J]. Journal of Cell Science, 2009, 122(17): 3123-3136.
[7] Huang S H, Duan S, Sun T, et al. JIP3 mediates TrkB axonal anterograde transport and enhances BDNF signaling by directly bridging TrkB with kinesin-1[J]. J Neurosci, 2011, 31(29): 10602-10614.
[8] 张磊. 复合异种骨的相关研究和进展[J]. 国外医学生物医学工程分册, 2004, 27(4): 242. ZHANG Lei. Improvement and study of complex-heterogeneity bone[J]. Foreign Medical Biomedical Engineering Fascicle, 2004, 27(4): 242.
[9] Hashizume H, Tamaki T, Oura H. Change in the extracellular matrix on the surface of sintered bovine bone implanted in the femur of a rabbit: an immunohistochemical study[J]. J Orthop Sci, 1998, 3(1): 42-53.
[10] Charalambides C, Beer M, Cobb A G. Poor results after augmenting autograft with xenograft (Surgihone) in hip revision surgery: a report of 27 cases[J]. Acta Orthop, 2005, 76(4): 544-549.
[11] 刘苏南, 郑启新. 异种骨移植的免疫反应及处理方法的研究进展[J]. 中华实验外科杂志, 2002, 19(1): 93. LIU Sunan, ZHENG Qixin. The research progress of immune response and treatment method of Xenogeneic Bone[J]. Chinese Journal of Experimental Surgery, 2002, 19(1): 93.
[12] 陈学英, 原林, 李靖, 等. 异种骨移植材料的制备与生物学评价[J]. 解剖学杂志, 2009, 32(2): 226-229. CHEN Xueying, YUAN Lin, LI Jing, et al. Preparation and study of xenogeneic bone graft[J]. Chinese Journal of Anatomy, 2009, 32(2): 226-229.
[13] Keskin D, Gundogdu C, Atac A C. Experimental comparison of bovine-derived xenograft, xenograft, xenograft-autologous bone marrow and autogenous bone graft for the treatment of bony defects in the rabbit ulna[J]. Med Princ Pract, 2007, 16(4): 299-305.
[14] Bi L, Hu Y, Fan H, et al. Treatment of contaminated bone defects with clindamycin-reconstituted bone xenograft-composites[J]. J Biomed Mater Res B Appl Biomater, 2007, 82(2): 418-427.
[1] LI Hongzhi, LIU Jing, SONG Yan, CHI Lingyi, LIU Yuguang. Role of liraglutide in the repair of spinal cord injury [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 1-5.
[2] YOU Jiebing, SUN Zhongwen, DU Juan, XU Wenjuan, WANG Yalin, LI Shuai, ZHU Meijia. Effects of rosiglitazone and insulin on the expression of TIPE2 and cell apoptosis in the brain of type 2 diabetic GK rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(4): 43-48.
[3] YANG Lu, LIU Yanguo, LI Jisheng, WANG Xiuwen. Effect of bufalin on chemosensitivity to cisplatin in non-small cell lung cancer and the underlying mechanisms [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(3): 6-11.
[4] DONG Ke1, PU Yedi2, LIU Qiong1, DAI Guangxia1, LI Lizhen1, LI Hao1, WANG Lingling1, WANG Luqun1. Inhibition of proliferation of human non-Hodgkins lymphoma Raji cells by small interference RNA silencing Pokemon gene [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 53-57.
[5] LIANG Ting-ting， ZHANG Ping， MA De-mei， ZHU Lin. Effect on apoptosis mediated by TRAIL against Caski cells of HPV16-E5-targeted siRNA [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(10): 24-28.
[6] . Effects of Gefitinib in combination with Celecoxib on apoptosis and expressions of EGFR and COX2 in cancerous lung cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(8): 37-41.
[7] WANG Chunfeng, JIANG Weiguo
. Effects of GnRHa and Gestrinone on growth and apoptosis
in ectopic endometrial stroma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(02): 70-73.
[8] ZHANG Jiao,LIU Qian,MAO Hai-ting,WANG Jie. Effect of interferon on the apoptosis in the hepatoma cell line HepG2.2.15 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(6): 582-585.
[9] RUAN Yong-wei,JIN Xing,HOU Lian-ze. Sequential chemotherapy of Epirubicin，Taxotere，and 5Fluorouracilincreases the apoptosis in breast cancer cell lines [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(7): 750-753.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd