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Effects of didanosine on the outgrowth of dissociated cultured dorsal root ganglion neuronal

LIU Hua-xiang1，LIU Zhen2，YANG Xiang-dong3，WANG Li-hong2，LI Zhen-zhong2   

  1. 1. Department of Rheumatology, Qilu Hospital of Shandong University;2. Department of Anatomy, School of Medicine, Shandong University；3. Department of Nephrology, Qilu Hospital of Shandong University
  • Received:2007-03-22 Revised:1900-01-01 Online:2007-12-24 Published:2007-12-24
  • Contact: LI Zhen-zhong

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Abstract: Objective To investigate the effects of didanosine (ddI) on the outgrowth of dissociated cultured dorsal root ganglion (DRG) neuronal. MethodsDissociated cultured DRG cells of the rat embryos were treated with ddI at different concentrations (1μg/mL, 5μg/mL, 10μg/mL and 20μg/mL, respectively). After 3 days exposure to ddI, the dissociated DRG cells were processed by microtubule associated protein 2 (MAP2) immunofluorescent labeling and the changes of neuronal cell body and neurites were observed by confocal laser scanning microscopy (CLSM). ResultsThe number of neurites in each neuron was decreased and the length of neurites in each neuron was shortened after 3 days treatment with ddI as compared with those in the control group. Whereas alterations of the diameter of neuronal cell bodies were not observed compared with those in the control group. ConclusionddI directly effects the outgrowth of neurites of the DRG neurons but dose not cause neuronal cell body shrinking in dissociated DRG cell cultures.

Key words: Didanosine, Ganglion, spinal, Neurons, Confocal laser scanning microscope

CLC Number: 

  • R322.85
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