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Different cell factor combinations on human bone marrow AC133+ and CD34+ cells ex vivo

ZHANG Chun-hua1，WEN Ze-qing1，ZHU Yong2   

  1. 1. Department of Obstetrics and Gynecology，Shandong Provincial Hospital, Shandong University； 2. Department of Surgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine
  • Received:2007-03-08 Revised:1900-01-01 Online:2007-10-24 Published:2007-10-24
  • Contact: WEN Ze-qing

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Abstract: Objective: To explore the effects of different cell factor combinations on the human bone marrow CD34+ cells and AC133+ cells ex vivo. Methods: The CD34+ cells and AC133+ cells were gathered by MiniMACS, then the cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, expansion of the CD34+ and AC133+　selection cells was observed and the clone formation of the CD34+ and AC133+ cells was also observed by using Half-solid Methyl cellulose under different cell factor stimulation (in7d, 14d, 21d.). The rate of cell apoptosis was determined. Results: The number the expansion folds and clones of the AC133+ selection cells were higher than those of the CD34+ selection cells under the same condition(P＜0.05).The rate of cell apoptosis from the AC133+ selection cells was lower than that from the CD34+ selection cells(P＜0.05). Conclusion: The AC133+ cells contain more hematopoietic progenitor cells than the CD34+ cells.

Key words: Bone marrow, Hematopoietic stem cells, AC133+ cells, CD34+ cells, Cytokines

CLC Number: 

  • Q254
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