红花如意丸对寒凝血瘀型子宫内膜异位症痛经患者疼痛的疗效及机制

doi:10.6040/j.issn.1671-7554.0.2024.0939

Abstract

Abstract: Objective To investigate the mechanism and analgesic effect of Honghua Ruyi Wan in treatments of dysmenorrhea in patients with cold congealing and blood stasis type endometriosis(EMs). Methods Sixty endometriosis patients from the First Affiliated Hospital and the Outpatient Department of Hebei University of Traditional Chinese Medicine participated in a randomized, double-blind, placebo-controlled trial. They were divided into two groups: a treatment group(30 cases)and a control group(30 cases). Honghua Ruyi Wan was orally administered to the treatment group, while Honghua Ruyi Wan simulator was administered to the control group. The numeric rating scale(NRS)score was assessed before treatment, and after one month and three months of treatment, as well as after three months of follow-up. Before and after three months of treatment, 5 mL of venous blood was collected on a fasting stomach, and the supernatant was extracted using centrifugation. Serum prostaglandin PGE2, PGF2α, vasodilator-stimulated phosphoprotein(VASP), fatty acid-binding protein 5(FABP5), vinculin(VCL), and melanoma cell adhesion molecule(MCAM)were determined by enzyme-linked immunosorbent test. Results Following one and three months of treatment and a three-month follow-up, the NRS scores of the patients in the treatment group were lower than those before treatment(P<0.01)and lower than those in the control group(P<0.01). After treatment, the PGF2α and MCAM levels in the treatment group were lower than those before treatment(P<0.05)and lower than those in the control group(P<0.05); the contents of PGE2, VCL, FABP5, and VASP were higher than those before treatment and those of the control group(P<0.05). The NRS score had a positive correlation with PGF2α and MCAM(P<0.001), but a negative correlation with PGE2, VASP, FABP5, and VCL(P<0.001). Conclusion In patients with cold coagulation and blood stasis EMs dysmenorrhea, the Honghua Ruyi Wan can reduce the NRS score, increase the serum levels of PGE2, VCL, FABP5 and VASP, and reduce the serum levels of PGF2α and MCAM, suggesting that Honghua Ruyi Wan may improve pelvic blood supply and nutrition through improving cell adhesion, morphology and function of vascular endothelial cells, improving blood flow, controlling inflammatory response, improving energy metabolism, and other mechanisms to treat dysmenorrhea in cold congealing and blood stasis type EMs.

Key words: Endometriosis, Prostaglandin, Melanoma cell adhesion molecule, Vasodilator-stimulated phosphoprotein, Fatty acid-binding protein 5, Vinculin

CLC Number:

R711

DU Yuhan, SUN Ying, DU Huilan. Effects and the mechanism of Honghua Ruyi Wan on dysmenorrhea in patients with cold congealing and blood stasis type endometriosis[J].Journal of Shandong University (Health Sciences), 2025, 63(10): 52-60.

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Effects and the mechanism of Honghua Ruyi Wan on dysmenorrhea in patients with cold congealing and blood stasis type endometriosis

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