Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (7): 98-103.doi: 10.6040/j.issn.1671-7554.0.2021.1454

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Clinical significance of Rh phenotype distribution and irregular antibody screening in RhD negative blood donors in Jinan of Shandong Province

ZHOU Na1,2, PAN Yingfang1, SHAO Jingru1, LI Jing1, SONG Yonghong1, XU Qun1, LYU Hongjuan1   

  1. 1. Blood Group Reference Laboratory, Shandong Blood Center, Jinan 250014, Shandong, China;
    2. Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-07-27

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Abstract: Objective To investigate the phenotype distribution and irregular antibody screening of RhD negative blood donors in Jinan, Shandong Province, so as to provide data support for rational collection and supply of RhD negative blood. Methods Blood samples of RhD negative blood donors were collected from Shandong Blood Center from July 2020 to June 2021 and then irregular antibody screening and Rh phenotype confirmation were conducted. Results Among the 877 RhD negative blood donors who were initially confirmed, 55 cases were RhD variants blood donors, and 822 were confirmed RhD negative blood donors by three kind of anti-D reagents, 6(0.730%)of which were irregular antibody positive. Antibodies detected included 4 anti-D antibodies, 1 anti-M antibody and 1 anti-Lea antibody. Positive rate of irregular antibody was much higher in female RhD negative blood donors than that in male. Conclusion Positive rate of irregular antibody in female RhD negative blood donors is much higher than that in male and the causes of antibody production are mostly natural and immune antibodies by pregnancy. Rh antigen phenotype detection and screening of irregular antibody for RhD negative blood donors are helpful for scientific and rational collection of RhD negative blood, which is of great significance for the clinical safety of blood transfusion.

Key words: RhD negative, Rh antigen phenotype, Irregular antibody, Blood donors, Rare blood database

CLC Number: 

  • R446
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