Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (4): 15-19.doi: 10.6040/j.issn.1671-7554.0.2019.032

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Natural killer cells regulate Th17/Treg cell immune response through modulating the function of dendritic cells during chlamydial lung infection

ZHOU Xiaoqing1, LI Jing1,2, ZHAO Lei3, TANG Wei1, YANG Xi1,4, WANG Hong1   

  1. 1. Department of Pathogenic Biology, Basic Science of Medical School, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Laboratory Medicine, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui, China;
    3. Institute of Basic Medical Science, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    4. Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg R3E 0T5, Canada
  • Published:2022-09-27

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Abstract: Objective To explore the mechanism of natural killer(NK)cells in regulating of Th17/Treg cell responses during chlamydia muridarum(Cm)lung infection. Methods A pulmonary infection model of Cm was established. The spleen dendritic cells(DCs)from Cm-infected mice with or without NK cells depletion were obtained by application of CD11c+ MicroBeads. The naïve mice were given intravenous injection of DCs from different groups of mice, and divided into NK-DCs recipients group(n=13)and NK+DCs recipients group(n=13). After adoptive transfer, the same infection method was used to recipient mice with Cm. The degree of disease was compared between the two groups. Flow cytometry was used to detect the changes of Th17 and Treg cells in the spleen and lung of mice. Results Compared with NK+DCs recipients, NK-DCs recipients exhibited aggravated disease, higher weight loss and severe lung injury. Immunological analysis showed the proportion of Th17 cells in spleen and lung was reduced while the proportion of Treg cell response was enhanced, which contributed to imbalance of Th17/Treg response in NK-DCs recipients. 山 东 大 学 学 报 (医 学 版)57卷4期 -周小青,等.自然杀伤细胞通过树突状细胞调控衣原体肺部感染中Th17/Treg免疫应答平衡 \=- Conclusion NK cells can effectively initiate Th17 cells response and inhibit Treg cell response by modulating DCs and thus play an important role in controlling chlamydial infection.

Key words: Chlamydia, Natural killer cells, Dendritic cells, Th17/Treg response, Lung infection

CLC Number: 

  • R374
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