Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (4): 15-19.doi: 10.6040/j.issn.1671-7554.0.2019.032

Previous Articles    

Natural killer cells regulate Th17/Treg cell immune response through modulating the function of dendritic cells during chlamydial lung infection

ZHOU Xiaoqing1, LI Jing1,2, ZHAO Lei3, TANG Wei1, YANG Xi1,4, WANG Hong1   

  1. 1. Department of Pathogenic Biology, Basic Science of Medical School, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Laboratory Medicine, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui, China;
    3. Institute of Basic Medical Science, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    4. Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg R3E 0T5, Canada
  • Published:2022-09-27

PDF (PC)

16

Abstract: Objective To explore the mechanism of natural killer(NK)cells in regulating of Th17/Treg cell responses during chlamydia muridarum(Cm)lung infection. Methods A pulmonary infection model of Cm was established. The spleen dendritic cells(DCs)from Cm-infected mice with or without NK cells depletion were obtained by application of CD11c+ MicroBeads. The naïve mice were given intravenous injection of DCs from different groups of mice, and divided into NK-DCs recipients group(n=13)and NK+DCs recipients group(n=13). After adoptive transfer, the same infection method was used to recipient mice with Cm. The degree of disease was compared between the two groups. Flow cytometry was used to detect the changes of Th17 and Treg cells in the spleen and lung of mice. Results Compared with NK+DCs recipients, NK-DCs recipients exhibited aggravated disease, higher weight loss and severe lung injury. Immunological analysis showed the proportion of Th17 cells in spleen and lung was reduced while the proportion of Treg cell response was enhanced, which contributed to imbalance of Th17/Treg response in NK-DCs recipients. 山 东 大 学 学 报 (医 学 版)57卷4期 -周小青,等.自然杀伤细胞通过树突状细胞调控衣原体肺部感染中Th17/Treg免疫应答平衡 \=- Conclusion NK cells can effectively initiate Th17 cells response and inhibit Treg cell response by modulating DCs and thus play an important role in controlling chlamydial infection.

Key words: Chlamydia, Natural killer cells, Dendritic cells, Th17/Treg response, Lung infection

CLC Number: 

  • R374
[1] Elwell C, Mirrashidi K, Engel J. Chlamydia cell biology and pathogenesis[J]. Nat Rev Microbiol, 2016, 14(6): 385-400.
[2] Brunham RC, Rey-Ladino J. Immunology of Chlamydia infection: implications for a Chlamydia trachomatis vaccine[J]. Nat Rev Immunol, 2005, 5(2):149-161.
[3] 吴移谋. 衣原体[M]. 北京: 人民卫生出版社, 2012.
[4] Bai H, Cheng J, Gao X, et al. IL-17/Th17 promotes type 1 T cell immunity against pulmonary intracellular bacterial infection through modulating dendritic cell function[J]. J Immunol, 2009, 183(9): 5886-5895.
[5] 陈彦波, 吴移谋. Th17细胞在衣原体感染中的作用研究[J]. 微生物学免疫学进展, 2015, 43(2): 45-48 CHEN Yanbo, WU Yimou. Role of Th17 cells in chlamydial infection[J]. Progress in Microbiology and Immunology, 2015, 43(2): 45-48.
[6] Zhang Y, Wang H, Ren J, et al. IL-17A synergizes with IFN-γ to upregulate iNOS and NO production and inhibit chlamydial growth[J]. PLoS One, 2012, 7(6): e39214. doi:10.1371/journal.pone.0039214.
[7] Labuda JC, McSorley SJ. Diversity in the T cell response to Chlamydia-sum are better than one[J]. Immunol Lett, 2018, 202: 59-64. doi:10.1016/j.imlet.2018.08.002.
[8] Stary G, Olive A, Radovic-Moreno AF, et al. VACCINES. A mucosal vaccine against Chlamydia trachomatis generates two waves of protective memory T cells[J]. Science, 2015, 348(6241): aaa8205. doi:10.1126/science.aaa8205.
[9] Noack M, Miossec P. Th17 and regulatory T cell balance in autoimmune and inflammatory diseases[J]. Autoimmun Rev, 2014, 13(6): 668-677.
[10] Yang H, Guo HZ, Li XY, et al. Viral RNA-unprimed RIG-I restrains Stat3 activation in the modulation of regulatory T cell/Th17 cell balance[J]. J Immunol, 2017, 199(1): 119-128.
[11] 胡亚南, 单永业, 罗亚文, 等. Th17/Treg比率在慢性乙型肝炎患者外周血的动态变化及意义[J]. 中国免疫学杂志, 2017, 33(7): 1072-1075. HU Yanan, SHAN Yongye, LUO Yawen, et al. Dynamic changes and significance of peripheral blood Th17/Treg in chronic hepatitis B patients[J]. Chinese Journal of Immunology, 2017, 33(7): 1072-1075.
[12] Li J, Dong X, Zhao L, et al. Natural killer cells regulate Th1/Treg and Th17/Treg balance in chlamydial lung infection[J]. J Cell Mol Med, 2016, 20(7): 1339-1351.
[13] 董晓婧, 李静, 赵蕾, 等. 鼠衣原体肺部感染中自然杀伤细胞对白细胞介素-22产生及疾病进程的影响[J]. 山东大学学报(医学版), 2016, 54(11): 19-23. DONG Xiaojing, LI Jing, ZHAO Lei, et al. Effect of natural killer cells on the production of interleukin-22 and disease process during Chlamydia muridarum lung infection[J]. Journal of Shandong University(Health Sciences), 2016, 54(11): 19-23.
[14] Zheng M, Sun R, Wei H, et al. NK cells help induce anti-hepatitis B virus CD8+ T cell immunity in mice[J]. J Immunol, 2016, 196(10): 4122-4131.
[15] Crouse J, Xu HC, Lang PA, et al. NK cells regulating T cell responses: mechanisms and outcome[J]. Trends Immunol, 2015, 36(1): 49-58.
[16] Jiao L, Gao X, Joyee AG, et al. NK cells promote type 1 T cell immunity through modulating the function of dendritic cells during intracellular bacterial infection[J]. J Immunol, 2011, 187(1): 401-411.
[17] Robbins SH, Bessou G, Cornillon A, et al. Natural killer cells promote early CD8 T cell responses against cytomegalovirus[J]. PLoS Pathog, 2007, 3(8): e123. doi:10.1371/journal.ppat.0030123.
[18] Moore-Connors JM, Fraser R, Halperin SA, et al. CD4+CD25+Foxp3+ regulatory T cells promote Th17 responses and genital tract inflammation upon intracellular Chlamydia muridarum infection[J]. J Immunol, 2013, 191(6): 3430-3439.
[19] Shekhar S, Peng Y, Gao XL, et al. NK cells modulate the lung dendritic cell-mediated Th1/Th17 immunity during intracellular bacterial infection[J]. Eur J Immunol, 2015, 45(10): 2810-2820.
[20] 赵国生, 徐晚雪, 王海萍, 等. 宿主的遗传背景对呼吸道感染沙眼衣原体后Treg产生的影响[J]. 中华微生物学和免疫学杂志, 2013, 33(3): 193-196. ZHAO Guosheng, XU Wanxue, WANG Haiping, et al. The different genetic background of inbred mouse strains affect Treg cells during Chlamydia muridarum lung infection[J]. Chinese Journal of Microbiology and Immunology, 2013, 33(3): 193-196.
[21] Mabey DC, Hu V, Bailey RL, et al. Towards a safe and effective chlamydial vaccine: lessons from the eye[J]. Vaccine, 2014, 32(14): 1572-1578.
[22] Cook KD, Waggoner SN, Whitmire JK. NK cells and their ability to modulate T cells during virus infections[J]. Crit Rev Immunol, 2014, 34(5): 359-388.
[23] Thomas R, Yang X. NK-DC crosstalk in immunity to microbial infection[J]. J Immunol Res, 2016, 2016: 6374379. doi:10.1155/2016/6374379.
[24] Andrews DM, Scalzo AA, Yokoyama WM, et al. Functional interactions between dendritic cells and NK cells during viral infection[J]. Nat Immunol, 2003, 4(2): 175-181.
[25] Ferlazzo G, Morandi B. Cross-talks between natural killer cells and distinct subsets of dendritic cells[J]. Front Immunol, 2014, 5: 159. doi:10.3389/fimmu.2014.00159.
[1] WANG Fuli, SUN Yinping, QIN Jie, RONG Jiansheng. Efficacy of DC-CIK cells combined with EGFR-TKI for 35 elderly patients with advanced EGFR-mutant lung cancer [J]. Journal of Shandong University (Health Sciences), 2022, 60(7): 110-117.
[2] XIANG Yujiao, LIU Qiang, LIU Lu, SHI Yan. Mechanism of abnormal immune response of dendritic cells in immune thrombocytopenia [J]. Journal of Shandong University (Health Sciences), 2022, 60(7): 89-97.
[3] LAN Jing, LI Dong, SHI Qing, LIU Zilin, ZHOU Panpan, JU Xiuli. Umbilical cord blood NK cells improved liver injury in sub-acute aging rats [J]. Journal of Shandong University (Health Sciences), 2018, 56(10): 64-71.
[4] DONG Xiaojing, LI Jing, ZHAO Lei, QI Mei, SUN Zeyu, WANG Hong, ZHAO Weiming. Effect of natural killer cells on the production of interleukin-22 and disease process during Chlamydia muridarum lung infection [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(11): 19-23.
[5] YANG Hui, DONG Rong, XUE Hui, HU Zuquan, ZENG Zhu. Gene microarray analysis of mature dendritic cells cultured with hepatocellular carcinoma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(1): 1-6.
[6] ZHUANG Yong, LI Dong, FU Jinqiu, SHI Qing, JU Xiuli. Biological features of dendritic cells derived from childhood B lineage acute lymphoblastic leukemia in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(11): 60-64.
[7] BIAN Xiao-kun1， SI Yuan-quan1， LU Nan3， LI Ying-Jie1， JIA Yan-fei2， ZHANG Yi1. Expression and clinical significance of immunoglobulin-like transcript 2 in gastric cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(1): 117-.
[8] YIN Xian-ming1, DONG Bai-hua1, ZHANG Yun2, SU Shi-li1, DONG Zhao-gang2, QU Xun2. Expression of integrin αvβ3 on NK cells of women with  early pregnancy or missed abortion [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(6): 140-.
[9] WANG He-li1, SUN Xue-mei2, SUN Zheng-yun1, WANG Na1, LIN Xia1. Changes of CD3+, CD4+ and CD8+ T cells and natural killer cells in neonates born to gestational diabetes mellitus mothers and  their relationship with blood glucose [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(4): 4-.
[10] NI Yong-liang, LI Qing. Clinical effectiveness of dendritic cells therapy combined with  intravesical  perfusion of epirubicin on bladder cancer after TURBT [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(2): 114-118.
[11] . [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(10): 18-22.
[12] CUI Yingying1,2，　ZHOU Ting2，　ZHANG Bei1,2，　RONG Fengnian2. Effect of DC-CIK pulsed with tumor lysate antigens of  SKOV3 on cytotoxic activity against SKOV3 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(2): 131-135.
[13] LI Bo1, ZHAO Qingli1, LUN Xiaoqin2, WEI Xuebin1, LI Shun1, LI Qing1

. Immunotherapeutic effect of dendritic cell vaccine modified with the CXCL10
gene and tumor cell RNA on mice with prostate cancer
 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(02): 10-13.
[14] MA Ming ,LI Xiu-zhen,WANG Chao,GUO Hong-min,MA Xin,ZHANG Xu. The MAGE-3-sensitized dendritic cell vaccine kills bladder cancer cells in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(5): 485-489.
[15] GAO Li-fen,SUN Wen-sheng,MA Chun-hong,ZHANG Li-ning,LIANG Xiao-hong,WANG Xiao-yan,GUO Chun,CHEN You-hai. Effects of transient transfection of pcDNA3-HBV on dendritic cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(4): 325-328.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd