Abstract: Objective To investigate the effect of resveratrol(Res)on the proliferation and cell cycle of human retinal capillary endothelium cells(HRCECs)in high glucose environment, and to explore its underlying mechanism. Methods HRCECs were divided into 5 groups with different treatment: Control group(N group)was treated with 5.5 mmol/L glucose; hypertonic group(M group)was treated with 5.5 mmol/L glucose and 19.5 mmol/L mannitol; high glucose control group(H group)was treated with 25 mmol/L glucose; experiment groups(R1-R4 groups)were treated with 25 mmol/L glucose and 25, 50, 100, 200 μmol/L Res. CCK-8 assay and flow cytometry were used to detect the effect of Res on the proliferation and cell cycle of HRCECs, respectively. Real-time PCR and Western blotting were used to detect the mRNA and protein levels of PCNA, Cyclin A1, CDK2 and P21^CIP1, respectively. Results The proliferation 山 东 大 学 学 报 (医 学 版)57卷3期 -崔锡铭,等.白藜芦醇对高糖环境下人视网膜血管内皮细胞增殖的影响及分子机制 \=-of HRCECs induced by high glucose was inhibited by Res in a concentration-dependent manner. Compared with H group, the cell numbers in S phase were increased in R2 and R3 groups(both P<0.01). Compared with N group, the mRNA and protein expressions of Cyclin A1, CDK2 and PCNA were increased while those of P21^CIP1 were decreased in H group(all P<0.01). Compared with H group, the expressions of Cyclin A1, CDK2 and PCNA in HRCRCs were decreased and those of P21^CIP1 were increased in R2 and R3 groups(all P<0.01). Conclusion Res can inhibit the high glucose-induced proliferation by down-regulating the expressions of Cyclin A1, CDK2 and PCNA and up-regulating the expression of P21^CIP1 in HRCECs.

Key words: Resveratrol, Human retinal capillary endothelium cells, Cycle-related proteins, Cell cycle