JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (3): 70-74.doi: 10.6040/j.issn.1671-7554.0.2016.1186

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Nucleoprotein gene analysis of measles virus in Guizhou Province in 2013

TANG Xiaomin1, ZHANG Li1, YE Xufang1, REN Gang1, ZUO Li2   

  1. 1. Department of Immunization Programs, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, Guizhou, China;
    2. Department of Immunology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China
  • Received:2016-09-20 Online:2017-03-10 Published:2017-03-10

Abstract: Objective To analyze the nucleoprotein genes of measles virus in Guizhou Province in 2013. Methods The throat swab, urine and sera samples were collected. Virus RNA was extracted directly from clinical specimens or cultured positive product of Vero/SLAM cell separation. Fragments of 634 nucleotide acids at the carboxyl end of the nucleoprotein gene were amplified by RT-PCR and then the PCR products were sequenced and analyzed. Results A total of 41 strains of measles virus were detected from 74 suspected measles cases. The results of phylogenetic analysis showed that 39 strains of measles virus were identified as H1a sub-genotype of H1 genotype, and located in two obvious branches. Two strains of them were identified as measles vaccine strain(A genotype). The nucleotide and amino acid divergences between 39 strains of measles virus strains and H1 genotype reference strain MVi/Hunan CHN/0.93/7(AF045212)were 2.0%-2.9% and 2.0%-4.7%, respectively. The nucleotide and amino acid divergences between 39 strains of measles virus strains and WHO H1a sub-genotype reference strain(Chin9322)were 0.9%-1.8% and 0.7%-3.4%, respectively. In addition, the nucleotide and amino acid homology among 39 MV strains of H1a sub-genotype were 97.3%-100% and 96.6%-100%, respectively. The nucleotide and amino acid homology between 2 strains of measles vaccine strains and S191 were 99.8%-100% and 99.3%-100%, respectively. Conclusion H1a sub-genotype was the predominant genotype in Guizhou Province in 2013, and there existed different chain of transmission in one year.

Key words: Genotype, Sequence analysis, Nucleoprotein, Measles virus

CLC Number: 

  • R373.11
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