JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (7): 6-10.doi: 10.6040/j.issn.1671-7554.0.2015.1247

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Expression and clinical significance of ARNT2 in gastric carcinoma

HAO Shuhua, JIA Yanfei, ZHENG Yan, MA Xiaoli, KONG Yi, LI Ping, ZONG Shuai, ZHANG Yao, WANG Yunshan   

  1. Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Received:2015-12-07 Online:2016-07-10 Published:2016-07-10

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Abstract: Objective To investigate the expression of aryl hydrocarbon receptor nuclear translocator 2(ARNT2)in human gastric carcinoma and its clinical significance. Methods The mRNA and protein expression levels of ARNT2 in normal gastric cell line and several gastric cancer cell lines were measured by quantitative real-time reverse transcription-polymerase chain reaction(qRT-PCR)and Western blotting. The expressions of ARNT2 protein in 61 cases of gastric cancer tissues and adjacent non-tumor tissues were detected by immunohistochemistry. Results The mRNA and protein expressions of ARNT2 in gastric cancer cell lines were significantly lower than those in normal gastric cell line. The positive expression of ARNT2 in gastric cancer tissues was lower than that in adjacent non-tumor tissues(32.79% vs 80.33%, P<0.05). Furthermore, ARNT2 expression was closely correlated with the differentiation of gastric cancer(P<0.05). Conclusion The expression of ARNT2 is lower in gastric cancer cells and tissues than in non-tumor cells and tissues, and is correlated with the differentiation of gastric cancer, which suggests that ARNT2 may participate in the occurrence and development of gastric cancer.

Key words: Gastric carcinoma, Aryl hydrocarbon receptor nuclear translocator 2, Transcription factor

CLC Number: 

  • R735.2
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