microRNA519D与基质金属蛋白酶2在36例子痫前期患者胎盘组织中的表达及意义

doi:10.6040/j.issn.1671-7554.0.2019.1049

摘要/Abstract

摘要： 目的探讨microRNA519D(miR-519d)及基质金属蛋白酶2(MMP2)在子痫前期(PE)患者胎盘组织中的表达。方法采用实时定量PCR、免疫组化及Western blotting技术检测于2018年11月至2019年5月行剖宫产的16例子痫前期孕妇(PE组)、20例子痫前期重度孕妇(PE重度组)和35例正常孕妇(对照组)胎盘组织中miR-519d与MMP2的表达。结果对照组、PE组及PE重度组胎盘组织中miR-519d的表达量分别为0.992±0.092、1.550±0.119、2.698±0.278,3组中miR-519d表达差异有统计学意义(F=638.245,P<0.001);经过两两比较,各组间miR-519d表达差异有统计学意义(PE组vs对照组,T=18.226,P<0.001;PE重度组vs对照组,T=26.588,P<0.001;PE组vs PE重度组,T=16.636,P<0.001)。对照组、PE组及PE重度组胎盘组织中MMP2蛋白的表达量分别为0.844±0.047、0.458±0.039、0.273±0.012,3组中MMP2表达差异有统计学意义(F=1552.203,P<0.001);经过两两比较,各组间MMP2表达差异均有统计学意义(PE组vs对照组,T=28.823,P<0.001;PE重度组vs对照组,T=68.189,P<0.001;PE组vs PE重度组,T=18.442,P<0.001)。分析显示MMP2和miR-519d表达呈负相关(r=-0.907,P<0.001)。结论 miR-519d在PE患者胎盘组织中表达升高,MMP2在PE患者胎盘组织中表达降低,且随病情程度加重,下降更明显,两者可能共同参与PE的发病过程。

关键词: 子痫前期, 胎盘, 基质金属蛋白酶2, microRNA519D

Abstract: Objective To investigate the expressions of microRNA519D(miR-519d)and matrix metalloproteinase2(MMP2)in placental tissues of patients with pre-eclampsia(PE). Methods A total of 71 pregnant women in Tangshan Gongren Hospital from Nov. 2018 to May 2019 were divided into PE patients(PE group, n=16), severe PE patients(severe PE group, n=20), and normal pregnant women(control group, n=35). The expression levels of miR-519d and MMP2 were detected by real-time quantitative PCR, immunohistochemistry and Western blotting methods. Results The expression levels of miR-519d in placental tissues of control group, PE group and severe PE group were 0.992±0.092, 1.550±0.119, and 2.698±0.278, respectively, and there was statistical difference on the whole(F=638.245,P<0.001). The expression levels of miR-519d between each group were statistically different(PE group vs control group, T=18.226, P<0.001; severe PE group vs control group, T=26.588, P<0.001; PE group vs severe PE 山东大学学报 (医学版)58卷1期 -张炜悦,等.microRNA519D与基质金属蛋白酶2在36例子痫前期患者胎盘组织中的表达及意义 \=- group, T=16.636, P<0.001). The expression levels of MMP2 in placental tissues of control group, PE group and severe PE group were 0.844±0.047, 0.458±0.039, and 0.273±0.012, respectively, and there was statistical difference on the whole (F=1 552.203,P<0.001). The expression levels of miR-519d between each group were statistically different(PE group vs control group, T=28.823, P<0.001; severe PE group vs control group, T=68.189, P<0.001; PE group vs severe PE group, T=18.442, P<0.001). MMP2 and miR-519d expressions exhibited a negative correlation(r=-0.907, P<0.001). Conclusion The expression of miR-519d in placental tissue of PE patients is increased, while the expression of MMP2 is decreased with the exacerbation of PE. MMP2 and miR-519d may participate in the pathogenesis of PE.

Key words: Pre-eclampsia, Placenca, Matrix metalloproteinase2, microRNA519D

中图分类号:

R714.24

张炜悦, 李亚光, 刘恩令, 王伟杰, 魏靖文, 荆楠. microRNA519D与基质金属蛋白酶2在36例子痫前期患者胎盘组织中的表达及意义[J]. 山东大学学报 (医学版), 2020, 58(1): 54-59.

ZHANG Weiyue, LI Yaguang, LIU Enling, WANG Weijie, WEI Jingwen, JING Nan. Expression of microRNA519D and matrix metalloproteinase2 in placenta tissues of pre-eclampsia and its significance[J]. Journal of Shandong University (Health Sciences), 2020, 58(1): 54-59.

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