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山东大学学报 (医学版) ›› 2020, Vol. 58 ›› Issue (1): 54-59.doi: 10.6040/j.issn.1671-7554.0.2019.1049

• • 上一篇    

microRNA519D与基质金属蛋白酶2在36例子痫前期患者胎盘组织中的表达及意义

张炜悦1, 李亚光1, 刘恩令2, 王伟杰1, 魏靖文1, 荆楠1   

  1. 1. 华北理工大学研究生院, 河北 唐山 063210;2. 唐山市工人医院妇产科, 河北 唐山 063000
  • 发布日期:2022-09-27
  • 通讯作者: 刘恩令. E-mail:tsenling888@163.com
  • 基金资助:
    河北省卫健委重点科技研究计划(20190167)

Expression of microRNA519D and matrix metalloproteinase2 in placenta tissues of pre-eclampsia and its significance

ZHANG Weiyue1, LI Yaguang1, LIU Enling2, WANG Weijie1, WEI Jingwen1, JING Nan1   

  1. 1. Graduate School, North China University of Science and Technology, Tangshan 063210, Hebei, China;
    2. Department of Obstetrics and Gynecology, Tangshan Gongren Hospital, Tangshan 063000, Hebei, China
  • Published:2022-09-27

摘要: 目的 探讨microRNA519D(miR-519d)及基质金属蛋白酶2(MMP2)在子痫前期(PE)患者胎盘组织中的表达。 方法 采用实时定量PCR、免疫组化及Western blotting技术检测于2018年11月至2019年5月行剖宫产的16例子痫前期孕妇(PE组)、20例子痫前期重度孕妇(PE重度组)和35例正常孕妇(对照组)胎盘组织中miR-519d与MMP2的表达。 结果 对照组、PE组及PE重度组胎盘组织中miR-519d的表达量分别为0.992±0.092、1.550±0.119、2.698±0.278,3组中miR-519d表达差异有统计学意义(F=638.245,P<0.001);经过两两比较,各组间miR-519d表达差异有统计学意义(PE组vs对照组,T=18.226,P<0.001;PE重度组vs对照组,T=26.588,P<0.001;PE组vs PE重度组,T=16.636,P<0.001)。对照组、PE组及PE重度组胎盘组织中MMP2蛋白的表达量分别为0.844±0.047、0.458±0.039、0.273±0.012,3组中MMP2表达差异有统计学意义(F=1552.203,P<0.001);经过两两比较,各组间MMP2表达差异均有统计学意义(PE组vs对照组,T=28.823,P<0.001;PE重度组vs对照组,T=68.189,P<0.001;PE组vs PE重度组,T=18.442,P<0.001)。分析显示MMP2和miR-519d表达呈负相关(r=-0.907,P<0.001)。 结论 miR-519d在PE患者胎盘组织中表达升高,MMP2在PE患者胎盘组织中表达降低,且随病情程度加重,下降更明显,两者可能共同参与PE的发病过程。

关键词: 子痫前期, 胎盘, 基质金属蛋白酶2, microRNA519D

Abstract: Objective To investigate the expressions of microRNA519D(miR-519d)and matrix metalloproteinase2(MMP2)in placental tissues of patients with pre-eclampsia(PE). Methods A total of 71 pregnant women in Tangshan Gongren Hospital from Nov. 2018 to May 2019 were divided into PE patients(PE group, n=16), severe PE patients(severe PE group, n=20), and normal pregnant women(control group, n=35). The expression levels of miR-519d and MMP2 were detected by real-time quantitative PCR, immunohistochemistry and Western blotting methods. Results The expression levels of miR-519d in placental tissues of control group, PE group and severe PE group were 0.992±0.092, 1.550±0.119, and 2.698±0.278, respectively, and there was statistical difference on the whole(F=638.245,P<0.001). The expression levels of miR-519d between each group were statistically different(PE group vs control group, T=18.226, P<0.001; severe PE group vs control group, T=26.588, P<0.001; PE group vs severe PE 山 东 大 学 学 报 (医 学 版)58卷1期 -张炜悦,等.microRNA519D与基质金属蛋白酶2在36例子痫前期患者胎盘组织中的表达及意义 \=- group, T=16.636, P<0.001). The expression levels of MMP2 in placental tissues of control group, PE group and severe PE group were 0.844±0.047, 0.458±0.039, and 0.273±0.012, respectively, and there was statistical difference on the whole (F=1 552.203,P<0.001). The expression levels of miR-519d between each group were statistically different(PE group vs control group, T=28.823, P<0.001; severe PE group vs control group, T=68.189, P<0.001; PE group vs severe PE group, T=18.442, P<0.001). MMP2 and miR-519d expressions exhibited a negative correlation(r=-0.907, P<0.001). Conclusion The expression of miR-519d in placental tissue of PE patients is increased, while the expression of MMP2 is decreased with the exacerbation of PE. MMP2 and miR-519d may participate in the pathogenesis of PE.

Key words: Pre-eclampsia, Placenca, Matrix metalloproteinase2, microRNA519D

中图分类号: 

  • R714.24
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