山东大学学报 (医学版) ›› 2019, Vol. 57 ›› Issue (1): 55-61.doi: 10.6040/j.issn.1671-7554.0.2018.1089
• • 上一篇
陈欧1,2,李国勇3,刘爱红2,朱晓波2,陈少杰2,王一彪2
CHEN Ou1,2, LI Guoyong3, LIU Aihong2, ZHU Xiaobo2, CHEN Shaojie2, WANG Yibiao2
摘要: 目的 探讨运用网络药理学方法预测麻黄治疗哮喘的抗炎靶点及其相关信号通路,发现哮喘的发病机制。 方法 在TCMSP数据库中搜索并筛选麻黄的活性成分,运用PharmMapper数据库预测活性成分的作用靶点,并进行分子对接。应用cytoscape3.6.1软件构建麻黄活性成分-预测靶点网络,并对网络拓扑结构进行分析。TTD数据库中搜索抗炎靶点,建立蛋白互作网络,并与麻黄活性成分-预测靶点网络融合,筛选活性成分作用的抗炎靶点。构建麻黄抗炎靶点对抗哮喘的体内反应网络,筛选与哮喘发病相关的抗炎靶点。使用Enrichr数据库以及cytoscape3.6.1对预测的麻黄治疗哮喘的抗炎靶点进行KEGG生物通路富集分析。 结果 筛选出23个化合物,对应156个靶点蛋白,其中表皮活性生长因子受体(EGFR)、E选择素(SELE)、巨噬细胞迁移抑制因子(MIF)、有丝分裂原激活蛋白激酶14(MAPK 14)4个靶点可能是麻黄治疗哮喘的重要抗炎靶点。KEGG分析得到的与这些抗炎靶点相关的主要信号通路有上皮细胞信号通路等。 结论 EGFR、SELE、MIF、MAPK14可能是麻黄在哮喘治疗中发挥抗炎作用的主要靶点,控制哮喘发生和发展,延缓病情恶化,可考虑整体控制这些抗炎靶点以及信号通路网络,而不仅仅是针对单一途径、疾病的关键靶点。
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