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山东大学学报 (医学版) ›› 2022, Vol. 60 ›› Issue (2): 22-26.doi: 10.6040/j.issn.1671-7554.0.2021.1148

• 临床医学 • 上一篇    下一篇

脂肪酸转运蛋白1在多囊卵巢综合征患者颗粒细胞中的表达

宋甜,付琳琳,王秋敏,杨晓,王莹,边月红,石玉华   

  1. 山东大学生殖医学研究中心(山东大学附属生殖医院)生殖内分泌教育部重点实验室 山东省生殖医学重点实验室山东省生殖健康临床医学研究中心 国家辅助生殖与优生工程技术研究中心, 山东 济南 250012
  • 发布日期:2022-01-25
  • 通讯作者: 石玉华. E-mail:shiyuhua2003@126.com
  • 基金资助:
    国家重点研发计划(2018YFC1003202,2017YFC1001004);泰山学者工程专项经费(ts201712103)

Expression of fatty acid transport protein 1 in granulosa cells of patients with polycystic ovary syndrome

SONG Tian, FU Linlin, WANG Qiumin, YANG Xiao, WANG Ying, BIAN Yuehong, SHI Yuhua   

  1. Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University;
    Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University;
    Shandong Key Laboratory of Reproductive Medicine;
    Shandong Provincial Clinical Research Center for Reproductive Health;
    National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan 250012, Shandong, China
  • Published:2022-01-25

摘要： 目的 探讨脂肪酸转运蛋白1(FATP1)在多囊卵巢综合征(PCOS)患者颗粒细胞中的表达水平及临床意义。 方法 选取进行体外受精-胚胎移植(IVF-ET)的75例PCOS患者(PCOS组),根据体质量指数(BMI)≥25判定为肥胖的标准,分为PCOS正常体质量组(n=36)和PCOS肥胖组(n=39);同期62例因输卵管梗阻或男方因素助孕患者(对照组),分为对照正常体质量组(n=31)和对照肥胖组(n=31)。采用逆转录实时定量PCR(qRT-PCR)检测患者颗粒细胞中FATP1的表达,分析其在PCOS患者中的差异性表达。 结果 FATP1在PCOS组卵巢颗粒细胞中的表达高于对照组(P <0.001);FATP1在PCOS正常体质量组卵巢颗粒细胞中的表达高于对照正常体质量组(P<0.001); FATP1在PCOS肥胖组卵巢颗粒细胞中的表达高于对照肥胖组(P<0.001)。二分类Logistic回归分析结果显示,高FATP1表达水平(OR=4.242, 95%CI:2.025~8.883, P<0.001)、高抗苗勒氏管激素(AMH,OR=1.512, 95%CI:1.184~1.928, P<0.001)水平、 高黄体生成素/卵泡刺激素(LH/FSH,OR=4.301, 95%CI:1.265~15.304, P=0.02)比值和高睾酮(T,OR=1.059, 95%CI:1.003~1.117, P=0.038)水平是影响PCOS的危险因素。 结论 FATP1在PCOS患者颗粒细胞中表达增高,可能在PCOS发生发展中发挥重要作用。

关键词: 脂肪酸转运蛋白1, 多囊卵巢综合征, 颗粒细胞, 脂肪酸, 增殖

Abstract: Objective To investigate the expression of fatty acid transport protein 1(FATP1)in granulosa cells of patients with polycystic ovary syndrome(PCOS)and its clinical significance. Methods A total of 75 PCOS patients(PCOS group)undergoing in vitro fertilization-embryo transform(IVF-ET)were selected. According to the standard of body mass index(BMI)≥ 25 as obesity, the subjects were divided into normal BMI PCOS group(n=36)and obese PCOS group(n=39). Another 62 patients(control group)seeking assisted reproduction treatment due to tubal or male factors were divided into normal BMI control group(n=31)and obese control group(n=31). The expression of FATP1 in patients granulosa cells was detected with reverse transcription real-time quantitative PCR(qRT-PCR), and its differential expression in PCOS patients was analyzed. Results The expression of FATP1 was higher in PCOS group than in controls(P<0.001), higher in the normal BMI PCOS group than in normal BMI control group(P<0.001), and higher in obese PCOS group than in obese control group(P<0.001). Binary Logistic regression analysis showed that higher FATP1 level(OR=4.242, 95%CI: 2.025-8.883, P<0.001), higher anti-Mullerian hormone level(AMH, OR=1.512, 95%CI: 1.186-1.928, P<0.001), higher luteotropic hormone/follicle stimulating hormone ratio(LH/FSH, OR=4.401, 95%CI: 1.265-15.304, P=0.02), and higher testosterone level(T, OR=1.059, 95%CI: 1.003-1.117, P=0.038)were risk factors of PCOS. Conclusion The expression of FATP1 is increased in PCOS patients, suggesting that FATP1 may play an important role in the development of PCOS.

Key words: Fatty acid transport protein1, Polycystic ovary syndrome, Granulosa cells, Fatty acid, Proliferation

中图分类号: 

  • R711.75
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