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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (9): 66-72.doi: 10.6040/j.issn.1671-7554.0.2017.024

• 基础医学 • 上一篇    下一篇

人血管基质片段联合脂肪干细胞促进裸鼠放射性皮肤损伤的愈合

朱琳1,李薇薇2,刘志凯3   

  1. 1.中国医学科学院北京协和医学院, 北京协和医院整形外科, 协和转化医学中心, 北京 100730;2.清华大学附属北京清华长庚医院整形外科, 北京 102218;3.中国医学科学院北京协和医学院, 北京协和医院放疗科, 协和转化医学中心, 北京 100730
  • 收稿日期:2017-01-08 出版日期:2017-09-10 发布日期:2017-09-10
  • 通讯作者: 李薇薇. E-mail:mimilee2004@126.com E-mail:mimilee2004@126.com
  • 基金资助:
    国家自然科学基金青年基金(81601694)

Human stromal vascular fraction combined with adipose stem cells promotes the healing of radiation skin damage in nude mice

ZHU Lin1, LI Weiwei2, LIU Zhikai3   

  1. 1. Department of Plastic Surgery, Chinese Academy of Medical Sciences, Peking Union Medical College, Peking Union Medical College Hospital, Peking Union Translational Medical Center, Beijing 100730, China;
    2. Department of Plastic Surgery, Beijing Tsinghua Changgeng Hospital, Tsinghua University, Beijing 102218, China;
    3. Department of Radiotherapy, Chinese Academy of Medical Sciences, Peking Union Medical College, Peking Union Medical College Hospital, Peking Union Translational Medical Center, Beijing 100730, China
  • Received:2017-01-08 Online:2017-09-10 Published:2017-09-10

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摘要： 目的 评价人血管基质片段(SVF)联合脂肪干细胞(ASCs)移植对裸鼠放射性皮肤损伤愈合的影响。 方法 从健康成年女性脂肪抽吸物酶解获取SVF及ASCs。制备裸鼠放射性皮肤损伤模型后,随机选取SVF+ASCs、SVF、ASCs、对照组的创面给予干预。术后7、14 d观察创面愈合情况;术后第14天取材,行HE染色观察创面炎性反应,免疫组织化学染色计数CD31阳性细胞的分布,Masson染色观察胶原排列情况。 结果 SVF+ASCs组14 d时创面愈合完全,对照组创面仍有肉芽组织;ASCs、SVF组创面仍有部分区域未愈合。SVF+ASCs组炎性细胞计数较ASCs组、SVF组及对照组明显减少,微血管计数CD31细胞阳性率明显高于其余3组,Masson染色SVF+ASCs组胶原排列较其他3组整齐。 结论 SVF+ASCs联合移植,可促进裸鼠放射性皮肤损伤的愈合,具有良好的应用前景。

关键词: 血管基质片段细胞, 脂肪干细胞, 放射性皮肤损伤, 血管化, 裸鼠

Abstract: Objective To evaluate the effects of stromal vascular fraction(SVF)combined with adipose stem cells(ASCs)transplantation on the healing of radiation skin damage in nude mice. Methods SVF and ASCs were extracted from the fat tissues of healthy adult women. The wound models were randomly divided into 4 groups: SVF+ASCs group, SVF group, ASCs group and sham group. The healing of wounds was observed on day 7 and 14. On day 14, samples were collected. The inflammatory reaction was observed with HE dying. The distribution of CD31 positive cells was determined with immunohistochemical staining. The distribution of collagen was observed with Masson staining. Results On day 14, wounds of the SVF+ASCs group were completely healed; wounds of the control group still showed granulation tissues; wounds of ASCs and SVF groups were largely unhealed. The count of inflammatory cells was lower in SVF+ASCs group than in ASCs group, SVF group and control group. Count of CD31 cells was significantly higher in SVF+ASCs group than in the other 3 groups. The collagen arrangement was more organized in SVF+ 山 东 大 学 学 报 (医 学 版)55卷9期 -朱琳,等.人血管基质片段联合脂肪干细胞促进裸鼠放射性皮肤损伤的愈合 \=-ASCs group than in the other 3 groups. Conclusion The SVF+ASCs transplantation can promote the healing of radiation skin damage innude mice and may be widely spread in clinical practice.

Key words: Stromal vascular fraction, Adipose stem cell, Radiation skin damage, Vascularization, Nude mouse

中图分类号: 

  • R622
[1] 陈犹白,王瑶,曲宝林,等. 脂肪干细胞治疗放射性皮肤和软组织损伤的研究进展[J/CD]. 中华损伤与修复杂志,2016,11(5): 366-369. CHEN Youbai, WANG Yao, QU Baolin, et al. The therapeutic effect of adipose-derived stem cells on radiation-induced injury of skin and soft tissue[J]. Chin J Injury Repair and Wound Healing(Electric Edition), 2016, 11(5): 366-369.
[2] Maxson S, Lopez EA, Yoo D, et al. Concise review: role of mesenchymal stem cells in wound repair[J]. Stem Cells Transl Med, 2012, 1(2): 142-149.
[3] Yang Z, Lin Z, Qin H, et al. Preformed gelatin microcryogels as injectable cell carriers for enhanced skin wound healing[J]. Acta Biom, 2015, 25(10): 291-303.
[4] 徐翔,汪海滨,孙中生,等. 脂肪来源SVF联合PRP注射改善鼻唇沟的临床研究[J]. 中国美容整形外科杂志,2015,26(2): 72-75. XU Xiang, WANG Haibin, SUN Zhongsheng, et al. Improvement of nasolabial fold by combined injection of platelet rich plasma and adipose-derived stromal vascular fraction[J]. Chin J Aesthet and Plast Surg, 2015, 26(2): 72-75.
[5] Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies[J]. Tissue Eng, 2001, 7(2): 211-228.
[6] 朱琳,刘志飞,张星,等. 糖尿病人脂肪干细胞(ADSCs)的分离、培养及鉴定研究[J]. 医学研究杂志,2014,43(8): 63-66. ZHU Lin, LIU Zhifei, ZHANG Xing, et al. Culture and identification of DM-derived adipose stem cells[J]. J Med Res, 2014, 43(8): 63-66.
[7] 张琳,富泽龙,冯锐.人脂肪干细胞复合富血小板血浆治疗裸鼠放射性皮肤损伤的实验研究[J].医学研究杂志,2015,44(7): 57-61. ZHANG Lin, FU Zelong, FENG Rui. Effects of human adipose-derived stem cells -platelet-rich plasma composite on healing of radiation skin injury[J]. J Med Res, 2015, 44(7): 57-61.
[8] 刘宸,章宏伟,徐宁.同种异体富血小板血浆可增强糖尿病大鼠合成创面胶原[J]. 中国组织工程研究,2014,18(39): 6329-6334. LIU Chen, ZHANG Hongwei, XU Ning. Allogeneic platelet-rich plasma promotes wound collagen synthesis in diabetic rats[J]. Chin J Tissue Eng Res, 2014, 18(39): 6329-6334.
[9] 杨懿爱, 郑丹宁, 李青峰. 细胞移植在自体脂肪填充中的应用进展[J]. 组织工程与重建外科杂志, 2012, 8(2): 106-109. YANG Yiai, ZHENG Danning, LI Qingfeng. Regenerative cell therapy in autologous fat transfer[J]. J Tissue Eng and Reconstruct Surg, 2012, 8(2): 106-109.
[10] 胡大海. 加强创面处理领域的干细胞研究[J]. 中华烧伤杂志, 2010, 26(4): 247-249. HU Dahai. Expediting the study on the role of stem cells in wound treatment[J]. Chin J Burn, 2010, 26(4): 247-249.
[11] 张兴安,吴蜀江,卢海彬,等. 自体富血小板血浆修复面神经损伤[J]. 中国组织工程研究,2013,17(28): 5145-5150. ZHANG Xingan, WU Sujiang, LU Haibin, et al. Autologous platelet rich plasma repair facial nerve injury[J]. Chin J Tissue Eng Res, 2013, 17(28): 5145-5150.
[12] 戴闽,周通华,程细高,等. 静磁场作用下金属离子对成骨细胞的生物学影响[J]. 天津医药,2011,39(8): 727-729. DAI Min, ZHOU Tonghua, CHENG Xigao, et al. The bio-effect of metal ions on osteoblasts under the static magnetic field[J]. Tianjin Med J, 2011, 39(8): 727-729.
[13] 李劼,高建华,鲁峰,等. 脂肪组织来源干细胞提高游离脂肪移植存活率的研究[J]. 中华整形外科杂志,2009,25(2): 129-133. LI Jie, GAO Jianhua, LU Feng, et al. Experimental study of the effect of adipose tissue derived stem cells on the survival rate of free fat transplantation[J]. Zhonghua Zheng Xing Wai Ke Za Zhi, 2009, 25(2): 129-133.
[14] 付冰川,高建华,鲁峰,等. 新鲜分离的脂肪SVF细胞促进脂肪移植存活的实验研究[J]. 中华整形外科杂志,2010,26(4): 289-294. FU Bingchuan, GAO Jianhua, LU Feng, et al. Experimental study of the effect of adipose stromal vascular fraction cells on the survival rate of fat transplantation[J]. Chinese Journal of Plastic Surgery, 2009, 25(2): 129-133.
[15] 李立群,潘盛盛,倪彬婷,等. 人血管基质片段细胞标记、分化及胶原支架的相容性[J].中华整形外科杂志,2013,29(3): 214-217. LI Liqun, PAN Shengsheng, NI Binting, et al. Experimental study on fluorescent labeling, differentiation, and compatibility with collagen framework of human stromal vascular fraction cells[J]. Chinese Journal of Plastic Surgery, 2013, 29(3): 214-217.
[16] Rourke RW, White AE, Metcalf MD, et al. Hypoxia-induced inflammatory eytokine secretion in human adipose tissue stromovascular cells[J]. Diabetologia, 2011, 54(6): 1480-1490.
[17] Matsumoto D, Sato K, Takaki Y, et al. Cell-assisted lipotransfer: supportive use of human adipose—derived ceils for soft tissue augmentation with lipoinjection[J]. Tissue Eng, 2006, 12(12): 3375-3382.
[18] Klinger M, Lisa A, Klinger F, et al. Regenerative approach to scars, ulcers and related problems with fat grafting[J]. Clin in Plast Surg, 2015, 42(3): 345-352.
[19] Andrew N, James G, Derek A, et al. Stromal vascular fraction: a regenerative reality? Part 1: Current concepts and review of the literature[J]. J Plast Reconstr Aesthet Surg, 2015, 69(2): 170-179.
[20] 马海欢.干细胞在创面修复中的应用[J].中国美容整形外科杂志,2011,22(5): 257-260. MA Haihuan. The role of stem cells in wound healing[J]. Chin J Aesthet and Plast Surg, 2011, 22(5): 257-260.
[21] Zhang S, Dong Z, Peng Z, et al. Anti-aging effect of adipose-derived stem cells in a mouse model of skin aging induced by D-galactose[J]. PloS One, 2014, 9(5): e97573. doi: 10.1371/journal.pone.0097573.
[22] Marco K, Andrea L, Francesco K, et al. Regenerative approach to scars, ulcers and related problems with fat grafting[J]. Clin Plast Surg, 2015, 42(3): 345-352.
[23] Dong Z, Peng Z, Chang Q, et al. The survival condition and immunoregulatory function of adipose stromal vascular fraction(SVF)in the early stage of nonvascularized adipose transplantation[J]. PloS One, 2013, 8(11): e80364. doi: 10.1371/journal.pone.0080364.
[24] Casteilla L, Planat-Benard V, Laharrague P, et al. AdiposeVrd V-Benstromal cells: their identity and uses in clinical trials, an update[J]. World J Stem Cells, 2011, 3(4): 25-33.
[25] 李立群,高建华,鲁峰,等. 血管基质片段细胞携血管内皮生长因子对移植颗粒脂肪血管化的影响[J].中华整形外科杂志,2012,28(2): 122-126. LI Liqun, GAO Jianhua, LU Feng, et al. Experimental study of the effect of adipose stromal vascular traction cells with VEGF on the neovascularization of free fat transplantation[J]. Chinese Journal of Plastic Surgery, 2012, 28(2): 122-126.
[26] King A, Balaji S, Weswani SG, et al. The role of stem cells in wound angiogenesis. Adv in wound care(New Rochelle), 2014, 10(3): 614-625.
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