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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (9): 66-72.doi: 10.6040/j.issn.1671-7554.0.2017.024

• 基础医学 • 上一篇    下一篇

人血管基质片段联合脂肪干细胞促进裸鼠放射性皮肤损伤的愈合

朱琳1,李薇薇2,刘志凯3   

  1. 1.中国医学科学院北京协和医学院, 北京协和医院整形外科, 协和转化医学中心, 北京 100730;2.清华大学附属北京清华长庚医院整形外科, 北京 102218;3.中国医学科学院北京协和医学院, 北京协和医院放疗科, 协和转化医学中心, 北京 100730
  • 收稿日期:2017-01-08 出版日期:2017-09-10 发布日期:2017-09-10
  • 通讯作者: 李薇薇. E-mail:mimilee2004@126.com E-mail:mimilee2004@126.com
  • 基金资助:
    国家自然科学基金青年基金(81601694)

Human stromal vascular fraction combined with adipose stem cells promotes the healing of radiation skin damage in nude mice

ZHU Lin1, LI Weiwei2, LIU Zhikai3   

  1. 1. Department of Plastic Surgery, Chinese Academy of Medical Sciences, Peking Union Medical College, Peking Union Medical College Hospital, Peking Union Translational Medical Center, Beijing 100730, China;
    2. Department of Plastic Surgery, Beijing Tsinghua Changgeng Hospital, Tsinghua University, Beijing 102218, China;
    3. Department of Radiotherapy, Chinese Academy of Medical Sciences, Peking Union Medical College, Peking Union Medical College Hospital, Peking Union Translational Medical Center, Beijing 100730, China
  • Received:2017-01-08 Online:2017-09-10 Published:2017-09-10

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摘要： 目的 评价人血管基质片段(SVF)联合脂肪干细胞(ASCs)移植对裸鼠放射性皮肤损伤愈合的影响。 方法 从健康成年女性脂肪抽吸物酶解获取SVF及ASCs。制备裸鼠放射性皮肤损伤模型后,随机选取SVF+ASCs、SVF、ASCs、对照组的创面给予干预。术后7、14 d观察创面愈合情况;术后第14天取材,行HE染色观察创面炎性反应,免疫组织化学染色计数CD31阳性细胞的分布,Masson染色观察胶原排列情况。 结果 SVF+ASCs组14 d时创面愈合完全,对照组创面仍有肉芽组织;ASCs、SVF组创面仍有部分区域未愈合。SVF+ASCs组炎性细胞计数较ASCs组、SVF组及对照组明显减少,微血管计数CD31细胞阳性率明显高于其余3组,Masson染色SVF+ASCs组胶原排列较其他3组整齐。 结论 SVF+ASCs联合移植,可促进裸鼠放射性皮肤损伤的愈合,具有良好的应用前景。

关键词: 血管基质片段细胞, 脂肪干细胞, 放射性皮肤损伤, 血管化, 裸鼠

Abstract: Objective To evaluate the effects of stromal vascular fraction(SVF)combined with adipose stem cells(ASCs)transplantation on the healing of radiation skin damage in nude mice. Methods SVF and ASCs were extracted from the fat tissues of healthy adult women. The wound models were randomly divided into 4 groups: SVF+ASCs group, SVF group, ASCs group and sham group. The healing of wounds was observed on day 7 and 14. On day 14, samples were collected. The inflammatory reaction was observed with HE dying. The distribution of CD31 positive cells was determined with immunohistochemical staining. The distribution of collagen was observed with Masson staining. Results On day 14, wounds of the SVF+ASCs group were completely healed; wounds of the control group still showed granulation tissues; wounds of ASCs and SVF groups were largely unhealed. The count of inflammatory cells was lower in SVF+ASCs group than in ASCs group, SVF group and control group. Count of CD31 cells was significantly higher in SVF+ASCs group than in the other 3 groups. The collagen arrangement was more organized in SVF+ 山 东 大 学 学 报 (医 学 版)55卷9期 -朱琳,等.人血管基质片段联合脂肪干细胞促进裸鼠放射性皮肤损伤的愈合 \=-ASCs group than in the other 3 groups. Conclusion The SVF+ASCs transplantation can promote the healing of radiation skin damage innude mice and may be widely spread in clinical practice.

Key words: Stromal vascular fraction, Adipose stem cell, Radiation skin damage, Vascularization, Nude mouse

中图分类号: 

  • R622
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