富氢水对兔腰椎间盘退变的影响

doi:10.6040/j.issn.1671-7554.0.2016.731

摘要/Abstract

摘要： 目的探讨腹腔注射富氢生理盐水对腰椎间盘退变的影响。方法 36只新西兰大白兔随机分为空白对照组、模型对照组和模型实验组,每组12只。各组均行右后外侧肌间隙入路显露腰椎间盘,空白对照组不做其他处理;模型对照组和模型实验组采用髓核穿刺抽吸法造腰椎间盘退变模型。模型实验组行腹腔注射富氢生理盐水8 mL/kg,每3天1次;模型对照组腹腔注射等量的等渗盐水;空白对照组不做任何处理。分别在4、8周行X线检查和组织病理学评价;采用ELISA法检测椎间盘组织中肿瘤坏死因子(TNF-α)、白细胞介素1(IL-1)、基质金属蛋白酶-3(MMP-3)含量。结果腰椎间盘退变模型用富氢生理盐水治疗4、8周后,在影像学和组织病理学方面均有明显改善; 模型实验组椎间盘中的TNF-α、IL-1、MMP-3含量均明显低于模型对照组,高于空白对照组, 差异有统计学意义(P<0.001)。结论腹腔注射富氢生理盐水治疗兔腰椎间盘退变性疾病具有一定的积极作用。

关键词: 白细胞介素1, 肿瘤坏死因子, 富氢水, 基质金属蛋白酶-3, 腰椎间盘退变, 兔

Abstract: Objective To investigate the effects of hydrogen-rich saline for lumbar intervertebral disc degeneration(LIVDD)in rabbits by intraperitoneal injection. Methods A total of 36 New Zealand white rabbits were randomly divided into 3 groups: normal control group, model control group and model experimental group, with 12 rabbits in each group. The lumbar discs of all rabbits were revealed from the right posterior lateral muscle gap. No further procedures were performed in the normal control group. Models of LIVDD induced by puncture and aspiration were established in the other 2 groups. Rabbits in the model experimental group were intraperitoneally injected with 8 mL/kg hydrogen-rich saline, once every 3 days. Equivalent saline was injected in rabbits of the model control group in the same way, while no treatment was done to the normal control group. The treatment lasted for 8 weeks. X-ray and histopathological examinations were conducted at the 4th and 8th week respectively after treatment. The contents of tumor necrosis factor(TNF-α), interleukin-1(IL-1)and matrix metalloproteinases-3(MMP-3)in the intervertebral disc tissue were detected with ELISA method. Results Significant improvements in imaging and histopathological data were found in the model experimental group after 4 or 8 weeks of hydrogen-rich saline treatment. The contents of TNF-α, IL-1 and MMP-3 in the experimental group were significantly lower than those in the model control group(P<0.001), but higher than those in the normal control group(P<0.001). The contents of inflammatory mediators over time had statistical difference in the model control group(P<0.001). Conclusion The intraperitoneal injection of hydrogen-rich saline in the treatment of LIVDD in rabbits plays a certain periodic positive role.

Key words: Interleukin-1, Matrix metalloproteinases-3, Hydrogen-rich saline, Tumor necrosis factor, Lumbar intervertebral disc degeneration, Rabbit

中图分类号:

R681.5

樊彦伟,严冬雪,招友. 富氢水对兔腰椎间盘退变的影响[J]. 山东大学学报（医学版）, 2017, 55(3): 38-42.

FAN Yanwei, YAN Dongxue, ZHAO You. Effects of hydrogen-rich saline for lumbar intervertebral disc degeneration in rabbits[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 38-42.

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