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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (7): 13-18.doi: 10.6040/j.issn.1671-7554.0.2014.719

• 基础医学 • 上一篇    下一篇

益气活血养阴解毒方对放射性肺损伤中TGF-β1、TNF-α表达的影响

王静1, 高桂新2, 万云焱1, 刘庆华1, 姚周虹1, 林殿杰1   

  1. 1. 山东大学附属省立医院呼吸内科, 山东 济南 250021;
    2. 山东大学附属省立医院肿瘤科, 山东 济南 250021
  • 收稿日期:2014-10-17 修回日期:2015-03-27 出版日期:2015-07-10 发布日期:2015-07-10
  • 通讯作者: 林殿杰。E-mail:dianjielin@126.com;高桂新。E-mail:slggxin@163.com E-mail:dianjielin@126.com;slggxin@163.com
  • 基金资助:
    山东省自然科学基金(ZR2009CM063,2010ZRB141621B)

Effect of Yiqihuoxue-Yangyinjiedu recipe on expressions of TGF-β1 and TNF-α in radiation-induced lung injury

WANG Jing1, GAO Guixin2, WAN Yunyan1, LIU Qinghua1, YAO Zhouhong1, LIN Dianjie1   

  1. 1. Department of Respiratory Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Oncology Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2014-10-17 Revised:2015-03-27 Online:2015-07-10 Published:2015-07-10

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摘要： 目的 研究益气活血养阴解毒方对放射性肺损伤中转化生长因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)表达的影响, 探讨其对放射性肺损伤的防治作用及机制。方法 将小鼠随机分为对照组(n=21)、中药组(n=21)、照射组(n=21)、治疗组(照射+中药, n=21), 用直线加速器对后两组小鼠全肺进行单次照射(12 Gy), 分别于照射后1、24、72 h和2、4、8、24周处死小鼠, 取标本进行组织学观察、RT-qPCR及免疫组化检测, 观察小鼠组织学变化及不同时间点的TGF-β1、TNF-α的表达。结果 益气活血养阴解毒方可以改善放射性肺损伤。照射组TGF-β1、TNF-α的mRNA及蛋白水平表达均高于对照组及中药组(P<0.05);除照射后1、24 h外, 治疗组TGF-β1、TNF-α在其余各时间点的表达水平均低于照射组(P<0.05)。结论 益气活血养阴解毒方具有抑制照射刺激下TGF-β1、TNF-α的表达、减轻放射性肺损伤的作用, 其机制与抑制TGF-β1、TNF-α的表达有关。

关键词: 放射性肺损伤, 转化生长因子-β1, 肿瘤坏死因子-α, 益气活血养阴解毒方

Abstract: Objective To investigate the influence of traditional Chinese medicine, Yiqihuoxue-Yangyinjiedu recipe, on transforming growth factor-β1(TGF-β1) and tumor necrosis factor-α(TNF-α) in lung tissues of mice with radiation-induced lung injury, and to explore the effects and action mechanism of Yiqihuoxue-Yangyinjiedu recipe on preventing and treating radiation-induced lung injury. Methods Mice were randomly divided into control group (n=21), drug group (n=21), radiation group (n=21), and treatment group (radiation+drug, n=21). The latter two groups were irradiated at whole thorax with a dose of 12 Gy. The pathology of radiation-induced lung injury was monitored. The expressions of TGF-β1 and TNF-α were analyzed by RT-qPCR and immunohistochemistry at 1, 24, 72 h and 2, 4, 8, 24 week post-irradiation. Results The results of histology demonstrated that Yiqihuoxue-Yangyinjiedu recipe treatment ameliorated radiation-induced lung injury. Expressions of TGF-β1, TNF-α mRNA and protein in radiation group were higher than those in control group and drug group (P<0.05). Except at 1 h and 24 h, expressions of TGF-β1 and TNF-α in treatment group at each time point were lower than those in radiation group (P<0.05). Conclusion Yiqihuoxue-Yangyinjiedu recipe can inhibit the expressions of TGF-β1 and TNF-α, and may play an important role in preventing and treating radiation-induced lung injury, which may be related to inhibit the expressions of TGF-β1 and TNF-α.

Key words: Radiation-induced lung injury, Tumor necrosis factor-α, Transforming growth factor-β1, Yiqihuoxue-Yangyinjiedu recipe

中图分类号: 

  • R563.1
[1] Mazeron R, Etienne-Mastroianni B, Pérol D, et al. Predictive factors of late radiation fibrosis: a prospective study in non-small cell lung cancer[J]. Int J Radiat Oncol Biol Phys, 2010, 77(1): 38-43.
[2] Oh YT, Noh OK, Jang H, et al. The features of radiation induced lung fibrosis related with dosimetric parameters[J]. Radiother Oncol, 2012, 102(3):343-346.
[3] Arkovitz MS, Garcia VF, Szabó C, et al. Decreased pulmonary compliance is an early indicator of pulmonary oxygen injury[J]. J Surg Res, 1997, 67(2): 193-198.
[4] Ashcroft T, Simpson JM, Timbrell V. Simple method of estimating severity of pulmonary fibrosis on a numerical scale[J]. J Clin Pathol, 1988, 41(4): 467-470.
[5] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001, 25(4): 402-408.
[6] Friedrichs K, Gluba S, Eidtmann H, et al. Overexpression of p53 and prognosis in breast cancer[J]. Cancer, 1993, 72(12): 3641-3647.
[7] Hill RP, Zaidi A, Mahmood J, et al. Investigations into the role of inflammation in normal tissue response to irradiation[J]. Radiother Oncol, 2011, 101(1): 73-79.
[8] Kim JY, Kim YS, Kim YK, et al. The TGF-beta1 dynamics during radiation therapy and its correlation to symptomatic radiation pneumonitis in lung cancer patients[J]. Radiat Oncol, 2009, 4: 59.
[9] Sanderson N, Factor V, Nagy P, et al. Hepatic expression of mature transforming growth factor beta1 in transgenic mice results in multiple tissue lesions[J]. Proc Natl Acad Sci U S A, 1995, 92(7): 2572-2576.
[10] 黄莺, 叶燕青, 孙洁民.肺纤维化形成过程中大鼠肺成纤维细胞分泌TGF-βmRNA及HGF mRNA对肺泡II型上皮细胞的影响[J]. 重庆医学, 2010, 39(19): 2548-2549, 2552. HUANG Ying, YE Yanqing, SUN Jiemin. Effect of pulmonary fibroblast express TGF-β mRNA and HGF mRNA to II alveolar epithelial cell in the rat model of pingyangmycin induced pulmonary fibrosis[J]. Chongqing Medicine, 2010, 39(19): 2548-2549, 2552.
[11] Chung EJ, Hudak K, Horton JA, et al. Transforming growth factor alpha is a critical mediator of radiation lung injury[J]. Radiat Res. 2014, 182(3): 350-362.
[12] 高桂新, 王成祥.周平安教授治疗放射性肺炎的临床经验[J].北京中医药大学学报:中医临床版, 2009, 16(4): 24-25.
[13] Dong XR, Wang JN, Liu L, et al. Modulation of radiation-induced tumour necrosis factor-α and transforming growth factor β1 expression in the lung tissue by Shengqi Fuzheng injection[J]. Mol Med Rep, 2010, 3(4): 621-627.
[14] Zhang K, Yang S, Zhu Y, et al. Protection against acute radiation-induced lung injury: a novel role for the anti-angiogenic agent Endostar[J]. Mol Med Rep, 2012, 6(2): 309-315.
[15] Wang J, Xu HW, Li BS, et al. Preliminary study of protective effects of flavonoids against radiation-induced lung injury in mice[J]. Asian Pac J Cancer Prev, 2012, 13(12): 6441-6446.
[16] Xie CH, Zhang MS, Zhou YF, et al. Chinese medicine Angelica sinensis suppresses radiation-induced expression of TNF-alpha and TGF-beta1 in mice[J]. Oncol Rep, 2006, 15(6): 1429-1436.
[17] 谢丛华, 周云峰, 彭纲, 等.当归对小鼠放射性肺损伤过程中TNF-α水平的影响[J].中华放射肿瘤学杂志, 2005, 14(1): 59-63. XIE Conghua, ZHOU Yunfeng, PENG Gang, et al. Modulation by Angelia Sinensis the expression of tumor necrosis factor αin radiation-induced damage of the lung[J]. Chin J Radiat Oncol, 2005, 14(1): 59-63.
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