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山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (8): 12-16.doi: 10.6040/j.issn.1671-7554.0.2015.1223

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p38 MAPK信号通路调控人主动脉平滑肌细胞Ⅰ型和Ⅲ型胶原的表达

王晓琳1,周元丽2,孙伟3,李莉1   

  1. 1.山东大学附属济南市中心医院心内科, 山东 济南 250013;2.山东大学附属济南市中心医院保健科, 山东 济南 250013;3.济南大学, 山东省医学科学院医学与生命科学学院, 山东 济南 250062
  • 收稿日期:2015-12-03 出版日期:2016-08-10 发布日期:2016-08-10
  • 通讯作者: 李莉. E-mail:lili74@medmail.com.cn E-mail:lili74@medmail.com.cn
  • 基金资助:
    国家自然科学基金青年科学基金(81200211);山东省重点研发计划(2015GGA01027);山东省优秀中青年科学家科研奖励基金(2012BSA01018);济南市科技明星计划(20120144)

p38 MAPK signaling pathway regulates the expressions of type Ⅰ and Ⅲ collagens in human aortic smooth muscle cells

WANG Xiaolin1, ZHOU Yuanli2, SUN Wei3, LI Li1   

  1. 1. Department of Cardiology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    2. Department of Health, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    3. School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences, Jinan 250062, Shandong, China
  • Received:2015-12-03 Online:2016-08-10 Published:2016-08-10

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摘要： 目的 探讨p38 MAPK对人主动脉平滑肌细胞Ⅰ型和Ⅲ型胶原表达的影响。 方法 分别使用药理学抑制剂和siRNA干预细胞。药理学抑制剂试验细胞分为:(1) 正常对照组,平滑肌细胞完全培养基正常培养;(2) DMSO(p38 MAPK抑制剂溶剂)对照组,0.01% DMSO处理;(3) p38 MAPK抑制剂组,0.01% p38 MAPK抑制剂(20 mg/mL)处理。基因沉默试验细胞分为:(1) 静态对照组,平滑肌细胞完全培养基正常培养; (2) 阴性对照siRNA组,MACSfectin试剂转染阴性对照siRNA 48 h; (3) p38 MAPK siRNA组,MACSfectin试剂转染p38 MAPK siRNA 48 h。采用qRT-PCR法检测Ⅰ型和Ⅲ型胶原mRNA表达;采用Western blotting法检测Ⅰ型和Ⅲ型胶原蛋白表达以及p38 MAPK转染效率;荧光显微镜观察siRNA转染效率。 结果 与正常对照组相比,DMSO对照组细胞Ⅰ型和Ⅲ型胶原mRNA和蛋白表达无明显变化(P>0.05),p38 MAPK抑制剂组细胞Ⅰ型和Ⅲ型胶原mRNA和蛋白表达显著降低(P<0.05)。与静态对照组相比,阴性对照siRNA组细胞Ⅰ型和Ⅲ型胶原mRNA和蛋白表达无明显变化(P>0.05),p38 MAPK siRNA组细胞Ⅰ型和Ⅲ型胶原mRNA和蛋白表达显著降低(P<0.05)。 结论 p38 MAPK信号通路调控人主动脉平滑肌细胞Ⅰ型和Ⅲ型胶原的表达。

关键词: Ⅰ型胶原, p38 MAPK, Ⅲ型胶原, 人主动脉平滑肌细胞

Abstract: Objective To investigate the effects of p38 MAPK on the expressions of type Ⅰ and type Ⅲ collagens in human aortic smooth muscle cells(HASMCs). Methods HASMCs were treated with inhibitors and siRNAs respectively. Inhibitor test cells were divided into 3 groups: (1) normal control group(cells were cultured in smooth muscle cell complete medium); (2) DMSO control group(cells were treated with 0.01% DMSO); (3) p38 MAPK inhibitor group(cells were treated with 0.01% p38 MAPK inhibitor(20 mg/mL). Gene silencing test cells were divided into 3 groups: (1) static control group(cells were cultured in smooth muscle cell complete medium); (2) negative control siRNA group(cells were transfected with negative control siRNAs for 48 h using MACSfectin reagent); (3) p38 MAPK siRNA group(cells were transfected with p38 MAPK siRNAs for 48 h using MACSfectin reagent). The mRNA expressions 山 东 大 学 学 报 (医 学 版)54卷8期 -王晓琳,等.p38 MAPK信号通路调控人主动脉平滑肌细胞Ⅰ型和Ⅲ型胶原的表达 \=- of type Ⅰ and Ⅲ collagens were detected by qRT-PCR, and the protein expressions were detected by Western blotting. The siRNAs transfection efficiency was observed with fluorescence microscope. Results Compared with the normal control group, the mRNA and protein expressions of type Ⅰ and Ⅲ collagens were not obviously changed in the DMSO control group(P>0.05), while the expressions decreased significantly in the p38 MAPK inhibitor group(P<0.05). Compared with the static control group, the mRNA and protein expressions of type Ⅰ and Ⅲ collagens were not obviously changed in the negative control siRNA group(P>0.05), while the expressions decreased significantly in the p38 MAPK siRNA group(P<0.05). Conclusion p38 MAPK signaling pathway regulates the expressions of type Ⅰ and type Ⅲ collagens in HASMCs.

Key words: Type Ⅲ collagen, Human aortic smooth muscle cells, p38 MAPK, Type Ⅰ collagen

中图分类号: 

  • R541.4
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