miR-1/133对病毒性心肌炎小鼠心肌细胞离子通道表达的影响

doi:10.6040/j.issn.1671-7554.0.2015.1171

摘要/Abstract

摘要： 目的探讨miR-1/133是否参与病毒性心肌炎心肌细胞钾离子、钙离子通道基因表达的调节。方法建立Balb/c小鼠急性病毒性心肌炎模型。将小鼠分为对照组、心肌炎组、心肌炎+miR-1/133mimics组、心肌炎+miR-1/133 NC组,每组10只。采用苏木精-伊红染色法观察心肌形态学改变;采用 qRT-PCR法检测心肌中miR-1、miR-133及Kcnd2、Irx5、Kcnj2和α1c的相对表达量; 采用Western blotting法检测心肌中蛋白Kv4.2、Kir2.1、Cav1.2的相对表达量。结果苏木精-伊红染色显示对照组心肌细胞排列整齐,间质无炎性细胞浸润;心肌炎组与心肌炎+miR-1/133 NC组心肌细胞水肿、排列紊乱,炎性细胞浸润间质;心肌炎+miR-1/133mimics组心肌细胞排列较整齐,无细胞水肿,间质少量炎性细胞浸润。与对照组相比,心肌炎组与心肌炎+miR-1/133 NC组心肌miR-1、miR-133及Kcnd2、Kcnj2表达下调,蛋白Kv4.2、Kir2.1的表达下调(P<0.01);Irx5、α1c及蛋白Cav1.2表达均上调(P<0.01);心肌炎+miR-1/133 mimics组较心肌炎组与心肌炎+miR-1/133 NC组相比,miR-1、miR-133及Kcnd2、Kcnj2表达上调,蛋白Kv4.2、Kir2.1表达上调(P<0.05),Irx5、α1c及蛋白Cav1.2表达均下调(P<0.01)。结论 miR-1/133参与病毒性心肌炎心肌细胞钾离子和钙离子通道基因表达的调节。

关键词: 钾通道, 钙通道, 基因表达, 微小RNA-1, 微小RNA-133, 病毒性心肌炎

Abstract: Objective To explore the involvement of miR-1/133 in regulating the expression of potassium and calcium ion channel in mouse with acute viral myocarditis. Methods After the mice models of acute viral myocarditis were established, they were divided into 4 groups: control group, viral myocarditis group, myocarditis+miR-1/133 mimics group, and myocarditis+miR-1/133 NC group, with 10 mice in each group. The myocardial pathological changes were observed with HE staining. The expressions of miR-1, miR-133, Kcnd2, Irx5, Kcnj2, and α1c in myocardium were detected with qRT-PCR. The expressions of Kv4.2, Kir2.1, and Cav1.2 in myocardium were detected with Western blotting. Results HE staining showed that, in the control group, myocardial cells arranged orderly, and no inflammatory cells infiltrated myocardium matrix; in the myocarditis group and myocarditis+miR-1/133 NC group, myocardial cells swelled and arranged disorderly, and inflammatory cells infiltrated myocardium matrix; in the myocarditis+miR-1/133 mimics group, myocardial cells arranged orderly without cell edema, and few inflammatory cells infiltrated myocardium matrix. Compared with the control group, in the myocarditis group and myocarditis+miR-1/133 NC group, the expressions of miR-1, miR-133, Kcnd2, Kcnj2, Kv4.2 and Kir2.1 in the myocardium significantly decreased(P<0.01), while the expressions of Irx5, α1c and Cav1.2 increased(P<0.01). Compared with the myocarditis 山东大学学报 (医学版)54卷8期 -刘蒙蒙,等.miR-1/133对病毒性心肌炎小鼠心肌细胞离子通道表达的影响 \=-group and myocarditis+miR-1/133 NC group, in the myocarditis+miR-1/133 mimics group, the expressions of miR-1, miR-133, Kcnd2, Kcnj2, Kv4.2 and Kir2.1 in the myocardium were upregulated(P<0.05), while the expressions of Irx5, α1c and Cav1.2 were downregulated(P<0.01). Conclusion miR-1/133 is involved in the regulation of calcium and potassium ion channel gene expression in mice with acute viral myocarditis.

Key words: Potassium channel, miR-1, miR-133, Viral myocarditis, Calcium channel, Gene expression

中图分类号:

R725.4

刘蒙蒙,赵翠芬,孔清玉,蔡直锋,夏伟. miR-1/133对病毒性心肌炎小鼠心肌细胞离子通道表达的影响[J]. 山东大学学报（医学版）, 2016, 54(8): 6-11.

LIU Mengmeng, ZHAO Cuifen, KONG Qingyu, CAI Zhifeng, XIA Wei. miR-1/133 participates in the expression of ion channel genes of myocardial cells in myocarditis mice[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(8): 6-11.

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