血清S100B蛋白、神经元特异性烯醇化酶与新生儿低血糖脑损伤的相关性

doi:10.6040/j.issn.1671-7554.0.2015.1013

摘要/Abstract

摘要： 目的探讨新生儿血清S100B蛋白、神经元特异性烯醇化酶(NSE)水平与新生儿低血糖脑损伤(HBD)的关系。方法选取2014年10月至2015年9月低血糖新生儿48例,依据临床表现分为症状性低血糖组(n=22)和无症状低血糖组(n=26)。正常对照组选取同期健康足月新生儿40例。各组新生儿留取出生后24、72 h血清样本,采用Human S100B Elisa kit及酶标仪检测血清S100B水平;应用罗氏411电化学发光免疫分析仪测定血清NSE水平。各组新生儿均行脑电图检查。结果症状性低血糖组血糖水平低于无症状性低血糖组和正常对照组(P<0.01),无症状低血糖组血糖水平低于正常对照组(P<0.05);症状性低血糖组低血糖持续时间高于无症状低血糖组(P<0.01);24 h血清NSE和S100B水平表达症状性低血糖组均显著高于无症状低血糖组和正常对照组(P<0.01),无症状低血糖组高于正常对照组(P<0.05)。而各组间72 h血清NSE和S100B水平差异无统计学意义(P>0.05);24 h血清NSE和S100B与血糖水平呈显著负相关性(P<0.05),与低血糖持续时间呈显著正相关性(P<0.05);血清NSE与S100B水平呈显著正相关性(P<0.05)。结论血清S100B蛋白及NSE在新生儿低血糖脑损伤时水平升高,且与患儿血糖降低程度以及低血糖持续时间相关,可作为反映新生儿HBD的早期敏感指标。

关键词: 新生儿, S100B蛋白, 神经元特异性烯醇化酶, 低血糖脑损伤

Abstract: Objective To investigate whether the serum S100B protein and neuron-specific enolase(NSE)levels were associated with neonatal hypoglycemic brain damage(HBD). Methods A total of 48 hypoglycemic neonates admitted during Oct. 2014 and Sept. 2015 were selected as the experimental group, who were divided into 2 subgroups according to clinical manifestations and EEG examination: symptomatic hypoglycemic group(n=22)and asymptomatic hypoglycemic group(n=26). Another 40 healthy subjects served as the control group. Blood samples were collected from all neonates 24 and 72 hours after birth. The levels of serum S100B protein and NSE protein were measured with Human S100B Elisa kit and Cobas 411 electro-chemiluminescence immunoassay analyzer, respectively. EEG examination was performed after admission. Results The level of blood glucose in the symptomatic group was lower than that of asymptomatic group and control group(P<0.01), and that of the asymptomatic group was lower than the control 山东大学学报 (医学版)54卷4期 -臧丽娇,等.血清S100B蛋白、神经元特异性烯醇化酶与新生儿低血糖脑损伤的相关性 \=-group(P<0.05). The duration of hypoglycemia was longer in the symptomatic group than in the asymptomatic group(P<0.01). The symptomatic group had significantly increased levels of serum 24 h-NSE and 24 h-S100B than the other 2 groups(P<0.01), and the asymptomatic group had higher levels than the control group(P<0.05). There were no statistical differences in the levels of serum 72 h-NSE and 72 h-S100B among the 3 groups(P>0.05). The level of serum 24 h-NSE was negatively correlated with 24 h-S100B, positively correlated with the duration of hypoglycemia(P<0.05), and the serum NSE was positively correlated with S100B protein(P<0.05). Conclusion The levels of serum S100B protein and NSE elevate in neonatal HBD, which are closely associated with the severity and duration of hypoglycemia, suggesting that they may serve as early sensitive markers for the diagnosis of neonatal HBD.

Key words: Neonate, Hypoglycemic brain damage, S100B protein, Neuron-specific enolase

中图分类号:

R722.1

臧丽娇,仇杰,庄根苗,安丽. 血清S100B蛋白、神经元特异性烯醇化酶与新生儿低血糖脑损伤的相关性[J]. 山东大学学报（医学版）, 2016, 54(4): 51-54.

ZANG Lijiao, QIU Jie, ZHUANG Genmiao, AN Li. Correlation among serum S100B protein, NSE and neonatal hypoglycemic brain damage[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 51-54.

参考文献

[1] 宿军, 王莉. 新生儿低血糖性脑损伤研究进展[J]. 中国当代儿科杂志, 2011, 13(5): 446-451. SU Jun, WANG Li. Research progress in neonatal hypoglycemic brain injury[J]. Chinese Journal of Contemporary Pediatrics, 2011, 13(5): 446-451.
[2] Vijay K, Agarwal A. Neonatal hypoglycemia resulting in occipital cerebral injury[J]. Pediatr Radiol, 2010, 40(Suppl 1): 178.
[3] Rehni AK, Nautiyal N, Perez-Pinzon MA, et al. Hyperglycemia / hypoglycemia-induced mitochondrial dysfunction and cerebral ischemic damage in diabetics[J]. Metabolic Brain Disease, 2014, 30(2): 1-11.
[4] Chaves ML, Ferreira ED. Serum levels of S100B and NSE proteins in Alzheimers disease patients[J]. J Neuro inflammation, 2010, 7(1): 1-7.
[5] 邵肖梅, 叶鸿猖. 实用新生儿学[M]. 4版. 北京: 人民卫生出版社, 2011: 755-758.
[6] 刘晓燕. 临床脑电图学[M]. 北京: 人民卫生出版社, 2006: 562-575.
[7] Lang TF, Hussain K. Pediatric hypoglycemia[J]. Adv Clin Chem, 2014, 63: 211-245.
[8] Rozance PJ, Hay WW. Hypoglycemia in newborn infants: Features associated with adverse outcomes[J]. Biology of the Neonate, 2006, 90(90): 74-86.
[9] Cryer PE. Hypoglycemia, functional brain failure, and brain death[J]. J Clin Invest, 2007, 117(4): 868-870.
[10] 王广斌, 季泰令. S100B蛋白、NSE作为脑损伤标志物的研究进展[J]. 中国老年学杂志, 2011, 3(31): 890-892.
[11] Zimmer DB, Cornwall EH, Landar A. The S100 protein family: history, function, and expression[J]. Brain Res Bull, 1995, 37(4): 417-429.
[12] Kaya C, Atas A, Aksoy N, et al. Evaluation of pre-treatment and post-treatment S100B, oxidant and antioxidant capacity in children with diabetic ketoacidosis[J]. J Clin Res Pediatr Endocrinol, 2015, 7(2): 109-113.
[13] Goncalves CA, Leite MC, Nardin P. Biological and methomological features of the measurement of S100B, a putative marker of brain injury[J]. Clin Biochem, 2008, 41(10-11): 755-763.
[14] Chiaretti A, Barone G, Riccardi R, et al. NGF, DCX, and NSE upregulation correlates with severity and outcome of head trauma in children[J]. Neurology, 2009, 72(7): 609-616.
[15] Li J, Zhang H, Xie M, et al. NSE, a potential biomarker, is closely connected to diabetic peripheral neuropathy[J]. Diabetes Care, 2013, 36(11): 3405-3410.
[16] Korfias S, Papadimitriou A, Stranjalis G, et al. Serum biochemical markers of brain injury[J]. Mini Rev Med Chem, 2009, 20(9): 227-234.
[17] Berger RP, Adelson PD, Richich R, et al. Serum biomarkers after traumatic and hypoxemic brain injuries: insight into the biochemical response of the pediatrirc brain to inflicted brain injury[J]. Dev Neurosci, 2006, 28(4-5): 327-335.
[18] Calderon LM, Guyette FX, Doshi AA, et al. Combining NSE and S100B with clinical examination findings to predict survival after resuscitation from cardiac arrest[J]. Resuscitation, 2014, 85(8): 1025-1029.

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