PRP在颅骨缺损修复中血管化的实验研究

doi:10.6040/j.issn.1671-7554.0.2014.234

摘要/Abstract

摘要： 目的观察富血小板血浆(PRP)在颅骨缺损修复中血管化的作用.方法在Wistar大鼠颅骨正中缘备出1.0 cm×0.8 cm大小缺损,随机分为4组,模型组只植入煅烧骨,BMSCs组植入煅烧骨+BMSCs(简称复合体),对照组植入复合体+VEGF,PRP组植入复合体+PRP;观察各组大鼠4、8、12周在颅骨缺损修复中血管化情况并检测1、4周血清NO、SOD、GSH-px和MDA含量变化.结果术后4、8和12周新生血管面积比,PRP组(3.08±0.21)%、(4.41±0.32)%、(5.71±0.47)%,对照组(2.26±0.17)%、(3.75±0.29)%、(4.53±0.42)%,BMSCs组(1.47±0.19)%、(2.62±0.27)%、(3.45±0.35)%,模型组(0.99±0.18)%、(1.71±0.23)%、(2.02±0.39)%,PRP组新生血管多,面积大,血管化情况明显优于模型组、BMSCs组(P<0.01)和对照组(P<0.05);PRP组NO、GSH-px和SOD的活性显著增高,与新生血管形成呈正相关,MDA含量明显降低,与血管形成呈负相关(P<0.01).结论 PRP能促进新生血管形成,加快颅骨缺损的修复.

关键词: 血管化, 丙二醛, 颅骨缺损, 富血小板血浆, 一氧化氮

Abstract: Objective To observe the effect of platelet-rich plasma (PRP) on the vascularization in defects of rat skull. Methods Bone defect with the size of 1.0 cm×0.8 cm was established in rat skulls with dental drill. Wistar rats were randomly divided into 4 groups: model group (implanted with true bone ceramics), BMSCs group (implanted with true bone ceramics and BMSCs), control group (implanted with true bone ceramics, BMSCs and VEGF), and PRP group (implanted with true bone ceramics, BMSCs and PRP). In week 4, 8 and 12 after operation, new vascularization was observed. In week 1 and 4, the contents of nitric oxide (NO), superoxide dismutase (SOD), glutathion peroxidase (GSH-px) and malondialdehyde (MDA) in serum were detected. Results In week 4, 8 and 12 after operation, new vascularization of PRP group, control group, BMSCs group and PRP group were (3.08±0.21)%, (4.41±0.32)% and (5.71±0.47)%, (2.26±0.17)%, (3.75±0.29)% and (4.53±0.42)%, (1.47±0.19)%, (2.62±0.27)% and (3.45±0.35)%, and (0.99±0.18)%, (1.71±0.23)% and (2.02±0.39)%, respectively. The new vascularization of PRP group was significantly higher than that of the model group, BMSCs group (both P<0.01), and control group (P<0.05). NO, activity of GSH-px and SOD of PRP group significantly increased, which was positively correlated with vascularization. MDA content decreased markedly and negatively correlated with vascularization (P<0.01). Conclusion PRP can promote vascularization and bone repair in skull defects.

Key words: Skull defects, Platelet-rich plasma, Vascularization, Malondialdehyde, Nitric oxide

中图分类号:

R687.34

刘斌钰, 刘斌焰, 邢雁霞, 何引飞, 徐欣. PRP在颅骨缺损修复中血管化的实验研究[J]. 山东大学学报（医学版）, 2015, 53(2): 27-33.

LIU Binyu, LIU Binyan, XING Yanxia, HE Yinfei, XU Xin. Experimental study of platelet-rich plasma on the vascularization in skull defects[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 27-33.

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