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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (10): 77-80,95.doi: 10.6040/j.issn.1671-7554.0.2014.360

• 临床医学 • 上一篇    下一篇

多囊卵巢综合征患者卵泡液中GDF9和BMP15的表达及意义

哈灵侠1,2, 李向红1,2   

  1. 1. 宁夏医科大学总医院生殖医学中心, 宁夏 银川 750004;
    2. 宁夏回族自治区生殖与遗传重点实验室 生育力保持省部共建教育部重点实验室, 宁夏 银川 750004
  • 收稿日期:2014-06-01 修回日期:2014-09-12 出版日期:2014-10-10 发布日期:2014-10-10
  • 通讯作者: 哈灵侠。E-mail：hlxny@163.com E-mail:hlxny@163.com
  • 基金资助:
    宁夏回族自治区自然科学基金（NZ1231）

Expression and significance of GDF9 and BMP15 in follicular fluid of women with polycysticovary syndrome

HA Lingxia1,2, LI Xianghong1,2   

  1. 1. Center for Reproductive Medicine, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    2. Key Laboratory of Reproduction and Genetics, Key Laboratory of Fertility Preservation and Maintenance, Ningxia Medical University, Ministry of Education in Ningxia, Yinchuan 750004, Ningxia, China
  • Received:2014-06-01 Revised:2014-09-12 Online:2014-10-10 Published:2014-10-10

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摘要： 目的 探讨体外受精-胚胎移植（IVF-ET）周期多囊卵巢综合征（PCOS）患者卵泡液中卵源性因子-生长分化因子9（GDF9）和骨形态发生蛋白15（BMP15）的表达及对IVF结局的影响。方法 收集60例PCOS患者（PCOS组）及60例卵巢功能正常妇女（对照组），分别记录患者一般情况、获卵数、成熟卵子数、正常受精卵数、卵裂数、优质胚胎数和临床妊娠率。Western blotting 检测所有对象卵泡液中GDF9和BMP15蛋白的表达量。根据阴道B超是否见到孕囊将PCOS患者进一步分为妊娠组和非妊娠组，分析两组卵泡液中GDF9和BMP15蛋白的表达情况。结果 ①PCOS组与对照组比较，获卵数分别为（18.76±8.51）个和（11.23±4.29）个，差异有统计学意义（P<0.05）；成熟卵子率、受精率、卵裂率、优质胚胎率和临床妊娠率差异无统计学意义（P>0.05）。②PCOS组卵泡液中GDF9和BMP15的表达量分别为0.85±0.27和1.29±0.48，对照组卵泡液中GDF9和BMP15的表达量分别为1.33±0.24和1.94±0.57，两组比较差异均有统计学意义（P<0.05）。③PCOS患者妊娠组GDF9和BMP15的表达量分别为1.01±0.31和1.50±0.41，非妊娠组GDF9和BMP15的表达量分别为0.71±0.21和1.09±0.46，两组比较差异均有统计学意义（P<0.05）。结论 经促排卵刺激的PCOS 患者卵泡液中GDF9、BMP15蛋白表达低于正常对照组，其表达水平的低下可能影响卵母细胞质量和胚胎发育潜能；妊娠组卵泡液GDF9、BMP15蛋白表达高于未妊娠组，提示卵源性因子GDF9、BMP15高表达可能与良好的妊娠结局相关。

关键词: 多囊卵巢综合征, 骨形态发生蛋白15, 生长分化因子9, 体外受精-胚胎移植

Abstract: Objective To investigate the expression of growth differentiation factor9 (GDF9) and bone morphogenetic protein15 (BMP15) in follicular fluid of polycystic ovarian syndrome (PCOS) patients and the effects on the outcome of in vitro fertilization and embryo transfer (IVF-ET). Methods A total of 60 PCOS patients (PCOS group) and 60 women with normal ovarian function (control group) were collected. The general information, the numbers of oocytes, mature oocytes, fertilized oocytes, cleavage, high quality embryos, and the rate of clinical pregnancy were recorded. Western blotting was used to detect the expressions of GDF9 and BMP15 in the follicular fluid of all subjects. The PCOS group was further divided into pregnant and non-pregnant subgroups according to the presence of a gestational sac on ultrasound. The expressions of GDF9 and BMP15 were analyzed. Results ① The number of oocytes was 18.76±8.51 and 11.23±4.29 in the PCOS group and control group, with significant difference (P<0.05). The number ofmature eggs, fertilization rate, cleavage rate, good quality embryo and the clinical pregnancy rate between the two groups had no significant differences (P>0.05). ② The expressions of GDF9 and BMP15 were 0.85±0.27 and 1.29±0.48 in the PCOS group, and 1.33±0.24 and 1.94±0.57 in the control group, with significant difference (P<0.05). ③ The expressions of GDF9 and BMP15 were 1.01 ± 0.31 and 1.50 ± 0.41 in the pregnant subgroup, and 0.71±0.21 and 1.09±0.46 in the non-pregnant subgroup, with significant difference (P<0.05). Conclusion The expressions of GDF9 and BMP15 in follicular fluid in the PCOS group are lower than those of the control group, suggesting that the low expression may affect oocyte quality and embryo development potential. The expressions of GDF9 and BMP15 in follicular fluid of the pregnant subgroup are higher than those of the non-pregnant subgroup, suggesting that the high expression may be associated with a good pregnancy outcome.

Key words: Polycystic ovary syndrome, In vitro fertilization and embryo transfer, Growth differentiation factor 9, Bone morphogenetic protein 15

中图分类号: 

  • R711.6
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