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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (10): 25-28.doi: 10.6040/j.issn.1671-7554.0.2014.144

• 基础医学 • 上一篇    下一篇

阿奇霉素和地塞米松对烟曲霉孢子刺激A549细胞分泌ICAM-1和TNF-α的影响

吴凤娟1, 李陶2, 亓倩3, 李玉2   

  1. 1. 菏泽市立医院呼吸科, 山东 菏泽 274007;
    2. 山东大学齐鲁医院呼吸科, 山东 济南 250012;
    3. 山东大学医学院, 山东 济南 250012
  • 收稿日期:2014-03-17 修回日期:2014-06-10 出版日期:2014-10-10 发布日期:2014-10-10
  • 通讯作者: 李玉。E-mail：qlliyu@163.com E-mail:qlliyu@163.com

Effects of azithromycin and dexamethasone on the secretion of ICAM-1 and TNF-α in A549 cells stimulated with Aspergillus fumigatus conidia

WU Fengjuan1, LI Tao2, QI Qian3, LI Yu2   

  1. 1. Department of Pulmonary Disease, Heze Municipal Hospital, Heze 274007, Shandong, China;
    2. Department of Pulmonary Disease, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    3. School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-03-17 Revised:2014-06-10 Online:2014-10-10 Published:2014-10-10

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摘要： 目的 观察阿奇霉素和地塞米松对烟曲霉孢子刺激A549细胞分泌ICAM-1和TNF-α的影响。方法 以正常培养的A549细胞为对照，采用ELISA方法检测A549细胞与烟曲霉孢子共孵育4、24 h后细胞分泌ICAM-1和TNF-α的水平，并在细胞与孢子共孵育24 h基础上，分别加入阿奇霉素（0.5、2.5 mg/L）和地塞米松（0.5、2.5 mg/L），比较不同药物浓度对两种细胞因子水平的影响。结果 A549细胞与烟曲霉孢子共孵4、24 h后，细胞释放ICAM-1和TNF-α的浓度均较对照组明显升高（P<0.05）；与单纯烟曲霉孢子刺激相比，2.5 mg/L阿奇霉素明显抑制与烟曲霉孢子共孵的细胞分泌TNF-α（P<0.05），2.5 mg/L地塞米松明显抑制与烟曲霉孢子共孵的细胞分泌ICAM-1和TNF-α（P<0.05）。结论 阿奇霉素和地塞米松均可以抑制烟曲霉孢子诱导的炎性因子释放，减轻炎症反应及肺损伤。

关键词: A549细胞, 细胞因子, 地塞米松, 烟曲霉孢子, 阿奇霉素

Abstract: Objective To evaluate the effect of azithromycin and dexamethasone on the secretion of ICAM-1 and TNF-α in A549 cells stimulated with Aspergillus fumigatus conidia. Methods The concentrations of ICAM-1 and TNF-α were detected by ELISA when A549 cells were cultured alone and were cultured with Aspergillus fumigatus conidia for 4 and 24 hours. The normal cultured A549 cells were taken as control group. After being cultured with conidia for 24 hours, A549 cells were treatd with azithromycin (0.5, 2.5 mg/L) or dexamethasone (0.5, 2.5 mg/L) and then the ICAM-1 and TNF-α concentrations were detected by ELISA. Results After being cultured with Aspergillus fumigatus conidia for 4 and 24 hours, both ICAM-1 and TNF-α secreted in A549 cells significantly increased(P<0.05), compared with control group. Compared with the stimulation of Aspergillus fumigatus conidia alone, 2.5 mg/L azithromycin significantly inhibited the secretion of TNF-α in A549 cells incubated with Aspergillus fumigatus conidia for 24 hours(P<0.05). While, 2.5 mg/L dexamethasone significantly inhibited the secretion of both TNF-α (P<0.05) and ICAM-1 in A549 cells incubated with Aspergillus fumigatus conidia for 24 hours (P<0.05). Conclusion Azithromycin and dexamethasone can inhibit the secretion of ICAM-1 and TNF-α in A549 cells stimulated with Aspergillus fumigatus conidia and thus relieve the inflammatory response of lung.

Key words: Cytokines, Azithromycin, Dexamethasone, Aspergillus fumigatus conidia, A549 cells

中图分类号: 

  • R379.6
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