您的位置:山东大学 -> 科技期刊社 -> 《山东大学学报(医学版)》

山东大学学报(医学版) ›› 2014, Vol. 52 ›› Issue (9): 44-47.doi: 10.6040/j.issn.1671-7554.0.2014.217

• 基础医学 • 上一篇    下一篇

大黄素对角膜炎大鼠角膜组织NF-κB活化表达的影响及意义

陈国玲1, 任然2, 张英辉3, 宋玮1, 考欣1, 张晗1   

  1. 1. 山东大学第二医院眼科, 山东 济南 250033;
    2. 济南市第五人民医院放射科, 山东 济南 250022;
    3. 山东大学第二医院病案室, 山东 济南 250033
  • 收稿日期:2014-04-12 修回日期:2014-06-06 出版日期:2014-09-10 发布日期:2014-09-10
  • 基金资助:
    山东省教育厅科技计划项目(J08LH59)

Significance and effect of emodin on activation of nuclear factor-κB in corneas of keratitis rats

CHEN Guoling1, REN Ran2, ZHANG Yinghui3, SONG Wei1, KAO Xin1, ZHANG Han1   

  1. 1. Department of Ophthalmology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Department of Radiology, the 5th People's Hospital of Jinan, Jinan 250022, Shandong, China;
    3. Department of Medical Records, the Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2014-04-12 Revised:2014-06-06 Online:2014-09-10 Published:2014-09-10
  • Contact: 张晗。E-mail:zhanghan9157@gmail.com E-mail:zhanghan9157@gmail.com

摘要: 目的 观察大黄素对角膜炎大鼠角膜组织核因子-κB(NF-κB)活化表达的影响及意义。方法 制备角膜炎动物模型。模型制备前30 min,分别于治疗组和炎症组大鼠结膜下注射大黄素及溶剂。裂隙灯显微镜观察大鼠眼部变化;Western blotting检测评价角膜组织NF-κB活化表达;免疫组织化学法检测角膜组织细胞间黏附分子-1 (ICAM-1)蛋白的表达。结果 成功制备了角膜炎大鼠模型,大黄素可改善角膜炎大鼠的眼部炎症反应,减少角膜组织炎细胞浸润。治疗组κB抑制蛋白α(IκBα)在脂多糖(LPS)刺激后各时点的表达均高于同时点炎症组表达(P<0.01)。LPS刺激可促进ICAM-1在角膜组织的阳性表达,该作用可被大黄素部分抑制。结论 大黄素可部分抑制角膜炎大鼠角膜组织中NF-κB活化,有效减轻角膜炎症的损伤。

关键词: 核转录因子-κB, 细胞因子, 角膜炎, 大黄素

Abstract: Objective To investigate the significance and effect of emodin on the activation of nuclear factor-κB (NF-κB) in corneas of keratitis rats. Methods Pseudomonas aeruginosa lipopolysaccharide (LPS)-induced keratitis models were established in Wistar rats. Before LPS exposure (30 minutes), emodin and its vehicle were injected into subconjunctival tissues of rats in treatment group and inflammation group. At different time after LPS challenge, rats were examined with slit lamp microscope to observe the severity of ocular inflammation. The expression of inhibitor NF-κBα (IκBα) was determined with Western blotting, and intercellular adhesion molecule-1 (ICAM-1) was detected with immunohistochemical staining. Results The rat models of keratitis were successfully established. LPS could induce a severe corneal inflammatory response, which could be improved by emodin. IκBα level was significantly decreased after LPS exposure, and the degeneration of IκBα induced by LPS was markedly inhibited by pretreatment with emodin (P<0.01). Furthermore, the positive cells of ICAM-1 staining were significantly decreased in treatment group as compared with inflammation group. Conclusion LPS takes part in the activation of NF-κB in keratitis corneas, and emodin can partly inhibit this activation and result in therapeutic effect.

Key words: Emodin, Cytokines, Nuclear factor-κB, Keratitis

中图分类号: 

  • R772.2
[1] 胡铭洋, 彭燕. 大黄治疗重症急性胰腺炎的研究进展[J]. 现代消化及介入诊疗, 2013, 18(5): 305-309. HU Mingyang, PENG Yan. Research progress of Rhu-barb in treatment of severe acute pancreatitis[J]. Modern Digestion & Intervention, 2013, 18(5): 305-309.
[2] Li Z, Xia X, Zhang S, et al. Up-regulation of Toll-like receptor 4 was suppressed by emodin and baicalin in the setting of acute pancreatitis[J]. Biomed Pharmacother, 2009, 63(2): 120-128.
[3] 沈爱娟, 蔡宛如. 大黄素抗炎作用及对急性肺损伤治疗作用研究进展[J]. 浙江中医药大学学报, 2013, 37(10):1261-1264. SHEN Aijuan, CAI Wanru. The research progress on anti-inflammatory effects and the therapy to acute lung injury of emodin[J]. Journal of Zhejiang Chinese Medical University, 2013, 37(10):1261-1264.
[4] Kitano A, Saika S, Yamanaka O, et al. Emodin suppression of ocular surface inflammatory reaction[J]. Invest Ophthalmol Vis Sci, 2007, 48(11): 5013-5022.
[5] 陈国玲, 陈双, 肖瑛,等. 大黄素对急性角膜炎症反应中ICAM-1表达的影响及意义[J]. 山东大学学报: 医学版, 2009, 47(5): 31-33. CHEN Guoling, CHEN Shuang, Xiaoying, et al. Signification and the Effect of emodin on expression of ICAM-1 in corneas of acute keratitis[J]. Journal of Shandong University: Health Science, 2009, 47(5): 31-33.
[6] Girgis D O, Sloop G D, Reed J M, et al. A new topical model of Staphylococcus corneal infection in the mouse[J]. Invest Ophthalmol Vis Sci, 2003, 44(4): 1591-1597.
[7] Kalariya N M, Shoeb M, Reddy A B, et al. Piceatannol suppresses endotoxin-induced ocular inflammation in rats[J]. Int Immunopharmacol, 2013, 17(2): 439-446.
[8] Chang C Y, Fu E, Chiang C Y, et al. Effect of paeonol on tissue destruction in experimental periodontitis of rats[J]. Am J Chin Med, 2014, 42(2): 361-374.
[9] 彭笑怒, 李文军, 田辉. 抑制聚二磷酸腺苷核糖聚合酶1减轻尼古丁诱导的支气管上皮细胞炎症反应[J]. 山东大学学报: 医学版, 2013, 51(2): 37-43. PENG Xiaonu, LI Wenjun, TIAN Hui. Poly(ADP-ribose) polymerase 1 inhibition protects human bronchial endothelial cells against nicotine-induced inflammation response[J]. Journal of Shandong University: Health Science, 2013, 51(2): 37-43.
[10] Kim B H, Lee J M, Jung Y G, et al. Phytosphingosine derivatives ameliorate skin inflammation by inhibiting NF- κB and JAK/STAT signaling in keratincoytes and mice[J]. J Invest Dermatol, 2014, 134(4): 1023-1032.
[11] Gupta D, Du Y, Piluek J, et al. Ethyl pyruvate ameliorates endotoxin-induced corneal inflammation[J]. Invest Ophthalmol Vis Sci, 2012, 53(10): 6589-6599.
[12] Zhu X, Zeng K, Qiu Y, et al. Therapeutic effect of emodin on collagen-induced arthritis in mice[J]. Inflammation, 2013, 36(6): 1253-1259.
[1] 余桂芳,陈树娣,陈雪竹,侯开连,梁敏. miR-916a调控SOCS6促进HBx-HepG2细胞生长[J]. 山东大学学报(医学版), 2016, 54(12): 14-19.
[2] 袁冰,李冉冉,韩明勇. 恶性黑色素瘤调节肺组织微环境并促进肿瘤肺转移的实验研究[J]. 山东大学学报(医学版), 2016, 54(11): 13-18.
[3] 张明明,安永辉,韩彩莉,张瑛琪,马明,李娜,邹长鹏. CIK细胞联合光动力治疗中晚期食管癌的疗效观察[J]. 山东大学学报(医学版), 2016, 54(1): 38-41.
[4] 李秀华, 李晓丽, 段瑞生, 朱梅佳, 曹莉莉, 李衍滨, 王思, 岳龙涛, 马庆海, 刘菲. 1,25(OH)2D3诱导实验性自身免疫性重症肌无力大鼠免疫耐受的机制[J]. 山东大学学报(医学版), 2015, 53(8): 5-10.
[5] 张蓬, 岳龙涛, 李亨, 张民, 王聪聪, 段瑞生, 窦迎春. 血脂康对实验性自身免疫性神经炎的治疗潜能[J]. 山东大学学报(医学版), 2015, 53(2): 1-5.
[6] 冯青, 谭晓冬. L161982对大鼠实验性自身免疫性神经炎中巨噬细胞亚型变化的影响[J]. 山东大学学报(医学版), 2015, 53(10): 21-25.
[7] 杨永荣, 李俊, 梁烨, 李近都, 蓝家富, 李天资. TNF-α-238G/A基因多态性与口腔扁平苔藓的关系[J]. 山东大学学报(医学版), 2014, 52(S1): 10-11.
[8] 徐良志1,陈昆仑2. 大黄素对骨肉瘤细胞增殖的抑制及相关机制[J]. 山东大学学报(医学版), 2014, 52(6): 37-40.
[9] 赵燕1,王刚1,陈大方2,程宇航1,陈雪彦1 . 首发未治精神分裂症患者IL-6、IL-10和IL-12水平及影响因素[J]. 山东大学学报(医学版), 2014, 52(4): 70-73.
[10] 隋翔宇, 张相春, 张光永, 胡三元, 王延磊, 戴勇. 连环蛋白p120在炎性肺损伤中保护作用的初步研究[J]. 山东大学学报(医学版), 2014, 52(12): 41-44.
[11] 吴凤娟, 李陶, 亓倩, 李玉. 阿奇霉素和地塞米松对烟曲霉孢子刺激A549细胞分泌ICAM-1和TNF-α的影响[J]. 山东大学学报(医学版), 2014, 52(10): 25-28.
[12] 刘露1,张雯1,陈翰祥1,郑琳1,卢翌2,王红1,唐伟1,赵蔚明1. PGRN缺失型腹膜巨噬细胞对细菌脂多糖的体外炎症应答[J]. 山东大学学报(医学版), 2013, 51(3): 58-62.
[13] 周冬枝,刘文,李跃军,夏欣欣,王玉,刘永惠. 大黄素对急性放射性肠炎的疗效及抗自由基损伤的实验研究[J]. 山东大学学报(医学版), 2011, 49(6): 76-79.
[14] 崔莹1,单培彦1,侯桂华2,麻琳1,冯永平3. 老年情感障碍患者SSRIs类药物治疗前后血清细胞因子、皮质醇及认知功能的变化[J]. 山东大学学报(医学版), 2011, 49(3): 76-80.
[15] 彭洁1,韩雪莹2,胡晓燕3,邓景惕3,郭媛1. 白藜芦醇对外周血单个核细胞中IL-1β、IL-6、MCP-1及SOCS1/3 表达的影响[J]. 山东大学学报(医学版), 2011, 49(11): 64-.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!