CIK细胞联合光动力治疗中晚期食管癌的疗效观察

doi:10.6040/j.issn.1671-7554.0.2015.772

摘要/Abstract

摘要： 目的探讨细胞因子诱导的杀伤细胞(CIK)联合光动力(PDT)治疗中晚期食管癌的临床疗效及对生活质量的影响。方法将收治的63例中晚期食管癌患者随机分为2组,单纯CIK组33例,行单纯CIK细胞治疗;联合治疗组30例,CIK细胞联合PDT治疗;CIK细胞培养14 d,分3次安全回输患者体内,间隔1~2 d,每次回输细胞数量>1×10⁹个,活力>95%。联合治疗组: CIK细胞培养14 d,期间第12天按照5 mg/kg静脉注射光敏剂喜泊分注射液,第13天在胃镜引导下使用630 nm波长的激光进行照射,功率密度150 mW/cm²,持续30 min,能量密度270 J/cm²,第14 天回输CIK细胞。对所有患者进行临床症状缓解率、内镜下总有效率、免疫功能及生活质量评价。结果联合治疗组临床症状缓解率(93.3% vs 60.6%)及内镜下评价总有效率(90.0% vs 63.6% )均高于单纯CIK组(P<0.01,P<0.05);两组CD3⁺、CD3⁺ CD56⁺治疗前后差异有统计学意义(均P<0.05),且治疗后联合治疗组CD3⁺、CD3⁺ CD56⁺较单纯CIK组明显上升(P<0.05);联合治疗组生活质量提高率显著高于单纯CIK组(86.7% vs 33.3%,P<0.01)。结论 CIK细胞联合光动力治疗中晚期食管癌患者,对提高患者的近期临床疗效,增强免疫功能等具有较好的临床价值。

关键词: 细胞因子诱导的杀伤细胞, 食管癌, 临床疗效, 光动力治疗

Abstract: Objective To evaluate the clinical efficacy of cytokine-induced killer(CIK)cells and photodynamic therapy(PDT)in combination in the treatment of advanced esophageal cancer. Methods A total of 63 patients with advanced esophageal cancer were collected and randomly divided into two groups: CIK group(treated with CIK cells alone, n=33), and CIK+PDT group(treated with both CIK cells and PDT, n=30). For the CIK group, CIK cells were cultured for 14 days, and transfused for 3 times at an interval of 1~2 days, and the number of CIK cells was >1×10⁹, activity > 95%. For the CIK+PDT group, CIK cells were cultured for 14 days, administered with photofrin 5 mg/kg on day 12, irradiated by 630nm-laser under endoscopic guidance on day 13 for 30 min, power density being 150 mW/cm², energy density being 270 J/cm², and transfused on day 14. The remission rate of clinical symptoms, total effective rate, immune function and life quality of all patients were evaluated. Results The remission rate of clinical symptoms(93.3% vs 60.6%)and the total effective rate(90% vs 63.6%)were much higher in the CIK+PDT group than in the CIK group(P<0.01, P<0.05). There were significant differences in the percentages of CD3⁺, and CD3⁺ CD56⁺ T cells before and after treatment in the two groups(all P<0.05); and the increase was higher in the CIK+PDT group than in the CIK group(P<0.05). The increase of life quality was higher in the CIK+PDT 山东大学学报 (医学版)54卷1期 - 张明明,等.CIK细胞联合光动力治疗中晚期食管癌的疗效观察 \=-group than in the CIK group(86.7% vs 33.3%, P<0.01). Conclusion The CIK cells combined with PDT can improve the clinical efficacy, and enhance the immune function of patients with advanced esophageal cancer, and thereby have a good clinical value.

Key words: Photodynamic therapy, Esophageal cancer, Clinical curative effect, Cytokine-induced killer cells

中图分类号:

R735.1

张明明,安永辉,韩彩莉,张瑛琪,马明,李娜,邹长鹏. CIK细胞联合光动力治疗中晚期食管癌的疗效观察[J]. 山东大学学报（医学版）, 2016, 54(1): 38-41.

ZHANG Mingming, AN Yonghui, HAN Caili, ZHANG Yingqi, MA Ming, LI Na, ZOU Changpeng. Clinical efficacy of combined cellular immunotherapy and photodynamic therapy for advanced esophageal cancer[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(1): 38-41.

参考文献

[1] 秦莉, 王志华. CIK细胞的体外培养与细胞毒作用[J]. 医学分子生物学杂志, 2007, 4(1):82-85. QIN Li, WANG Zhihua. In vitro culture and cytotoxicity of cytokine-induced killer cells[J]. Journal of Medical Molecular Biology, 2007, 4(1):82-85.
[2] Yoon HY, Cheon YK, Choi HJ, et al. Role of photodynamic therapy in the palliation of obstructing esophageal cancer[J]. Korean J Intern Med, 2012, 27(3):278-284.
[3] 罗光华, 马鸣, 郭莉莉, 等. CIK 细胞治疗对肿瘤患者外周血免疫细胞亚型的影响[J]. 中国肿瘤生物治疗杂志, 2013, 20(4):475-477. LUO Guanghua, MA Ming, GUO Lili, et al. Effect of cytokine-induced killer cell therapy on immune cell subsets of peripheral blood in tumor patients[J]. Chinese Journal of Cancer Biotherapy, 2013, 20(4):475-477.
[4] 沙卫红, 李瑜元, 聂玉强, 等. 光动力疗法治疗进展期消化道肿瘤的疗效观察[J]. 中国综合临床, 2005, 21(2):125.
[5] 毛永平, 邱海霞, 顾瑛, 等. 进展期食管癌的光动力治疗[J]. 中国激光医学杂志, 2010, 19:10-12. MAO Yongping, QIU Haixia, GU Ying, et al. Photodynamic therapy for advanced esophageal cancer[J]. Chinese Journal of Laser Medicine & Surgery, 2010, 19:10-12.
[6] 周际昌. 实用肿瘤内科学[M]. 北京: 人民卫生出版社, 2003: 28-48.
[7] 李昌立. 氟尿嘧啶植入剂用于消化道恶性肿瘤手术患者效果观察[J]. 山东医药, 2012, 52: 59-60.
[8] Sangiolo D. Cytokine induced killer cells as promising immunotherapy for solid tumors[J]. J Cancer, 2011, 2(6):363-368.
[9] Milla Sanabria L, Rodriguez ME, Cogno IS, et al. Direct and indirect photodynamic therapy effects on the cellular and molecular components of the tumor micro environment [J]. Biochim Biophys Acta, 2013, 1835(1):36-45.
[10] 吴念, 冯虎翼. 细胞因子诱导的杀伤细胞免疫治疗的研究进展[J]. 中国生物制品学杂志, 2014, 6(27):861-864. WU Nian, FEN Huyi. Progress in research on immunotherapy with cytokine-induced killer cells[J]. Chin J Biologicals, 2014, 6(27):861-864.
[11] 李黎波, 罗荣城, 廖旺军, 等. 内镜下卟吩姆钠-光动力学疗法治疗恶性肿瘤的临床研究[J]. 中国激光医学杂志, 2007, 16(1): 27-30. LI Libo, LUO Rongcheng, LIAO Wangjun, et al. Endoscopic perfimer sodium-photodynamic therapy for malignant tumor: a clinical study[J]. Chinese Journal of Laser Medicine & Surgery, 2007, 16(1):27-30.
[12] 潘林娜, 张志燕, 李曼, 等. 光动力治疗食管癌近期疗效观察[J]. 世界华人消化杂志, 2006, 14(5):530-532. PAN Linna, ZHANG Zhiyan, LI Man, et al. Effect of photodynamic therapy for esophageal carcinoma in near future [J]. World Chinese Journal of Digestology, 2006, 14(5): 530-532.
[13] Yoon HY, Cheon YK, Choi HJ, et al. Role of photodynamic therapy in the palliation of obstructing esophageal cancer[J]. Korean J Intern Med, 2012, 27(3):278-284.
[14] Yoo J, Ha K. New insights into the mechanisms for photodynamic therapy-induced cancer cell death[J]. Int Rev Cell Mol Biol, 2012, 295:139-174.
[15] Peters FP, Kara MA, Rosmolen WD, et al. Endoscopic treatment of high-grade dysplasia and early stage cancer in Barretts esophagus[J]. Gastrointest Endosc, 2005, 61(4):506-514.
[16] Allison RR, Moghissi K. Oncologic photodynamic therapy: clinical strategies that modulate mechanisms of action[J]. Photodiagnosis Photodyn Ther, 2013, 10(4): 331-341.

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