关键词: 结直肠癌; MiR-182; 迁移

Abstract:

Objective   To detect the expression level of miR-182 in colorectal cancer, explore the correlations between miR-182 expression and clinicopathological features, and investigate its effect on the migration of colorectal cancer cells in vitro. Methods   Quantitative real-time PCR (qRT-PCR) was used to measure the expression of miR-182 in 86 pairs of colorectal cancer and corresponding adjacent noncancerous tissues, and the correlations between miR-182 expression and clinicopathological features were further analyzed． HT-29 cells were transiently transfected with miR-182 mimics and miR-negative control, and the experiment was divided into miR-182 over-expression group, negative control group and blank group. In addition, the migration ability was detected by transwell assay in vitro. Results   The expression of miR-182 was significantly higher in colorectal cancer tissues compared with corresponding adjacent noncancerous tissues (P<0.001). Its level was significantly correlated with local invasion (P=0.028), lymph nodes metastasis (P=0.003), distant metastasis (P=0.006), and clinical stage (P=0.005), respectively. However, no correlation was observed between miR-182 and age or other clinicopathological parameters (all P>0.05). The migration cells were significantly higher in miR-182 over-expression group than negative control group and blank group. Conclusion    MiR-182 is up-regulated in colorectal cancer and that high miR-182 expression is associated with the metastasis and progression of colorectal cancer.  In addition, the up-regulated miR-182 promotes colorectal cancer cell migration in vitro, suggesting a potential target for the prevention and therapy of metastasis in colorectal cancer.

Key words: Colorectal cancer; MiR-182; Migration