自体CIK细胞治疗对卵巢癌调节性T细胞的影响

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (5): 101-104.

自体CIK细胞治疗对卵巢癌调节性T细胞的影响

于渊1,李岩1,荣风年2,梁婧1,刘晓琳1,王福立1

山东大学附属千佛山医院 1. 肿瘤科；2. 妇产科, 济南 250014

收稿日期:2010-01-13 出版日期:2010-05-16 发布日期:2010-05-16
通讯作者: 李岩（1962- ），女,主任医师,主要从事肿瘤化疗及生物治疗的研究。 E-mail:liyan@sdhospital.com.cn
作者简介:于渊（1982- ），女,硕士研究生,主要从事肿瘤化疗及生物治疗的研究。
基金资助:
山东省自然科学基金资助课题（2008ZRB02001）

Therapy effect of auto-CIK on regulatory T cells in ovarian neoplasms patients

YU Yuan1, LI Yan1, RONG Fengnian2, LIANG Jing1, LIU Xiaolin1, WANG Fuli1

1. Department of Oncology；2. Department of Gynaecology and Obstetrics
Qianfoshan Hospital of Shandong University, Jinan 250014, China

Received:2010-01-13 Online:2010-05-16 Published:2010-05-16

摘要/Abstract

摘要：

目的观察细胞因子激活的杀伤细胞（CIK）对卵巢癌患者外周血CD4+CD25+调节性T细胞及T细胞亚群变化的影响。方法对30例经病理确诊为卵巢癌的患者,取其自体外周血单个核细胞,通过培养将其诱导为CIK细胞,然后将细胞进行自体回输。2周后采用流式细胞仪检测外周血CD4+CD25+调节性T细胞、T细胞亚群及NK细胞的变化。结果 CIK细胞输注后,患者外周血CD4+CD25+调节性T细胞减少(P＜0.01),CD8+下降(P＜0.05),CD3+、CD4+细胞、CD4+/CD8+比值及NK细胞较治疗前显著升高。结论卵巢癌患者经自体CIK细胞回输治疗后,外周血调节性T细胞的数量降低,T细胞亚群比例失调和功能低下的状况得到不同程度的改善。

关键词: 调节性T细胞；CIK细胞；免疫治疗；卵巢肿瘤

Abstract:

Objective To evaluate the variation of CD4+CD25+ regulatory T cells and T cell subsets affected by cytokine-induced killer cells in ovarian neoplasms patients. Methods Thirty ovarian neoplasms patients were collected with pathologically diagnosed. The auto-CIK cells were transfused back into patients after peripheral blood was cultured and induced into CIK cells. The levels of CD4+CD25+ regulatory T cells, T cell subsets and NK cells were detected with flow cytometry two weeks later. Results CD4+CD25+ regulatory T cells were decreased after autologous CIK cells transfusion, (P＜0.01); the CD8+ cell subsets were decreased (P＜0.05). Cell subsets CD3+ and CD4+, ratio of CD4+/CD8+ and NK cells were significantly increased at the same time. Conclusion Autologous CIK cells can degrade the quantity of regulatory T cells, improve the unbalanced T cell subsets in patients with ovarian neoplasms and upgrade the quality of patients′ life to some extent.

Key words: Regulatory T cells；Cytokine induced killer cells；Immunotherapy；Ovarian neoplasms

于渊1,李岩1,荣风年2,梁婧1,刘晓琳1,王福立1. 自体CIK细胞治疗对卵巢癌调节性T细胞的影响[J]. 山东大学学报(医学版), 2010, 48(5): 101-104.

YU Yuan1, LI Yan1, RONG Fengnian2, LIANG Jing1, LIU Xiaolin1, WANG Fuli1. Therapy effect of auto-CIK on regulatory T cells in ovarian neoplasms patients[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(5): 101-104.

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