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山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (5): 105-.

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IL-17在子痫前期患者胎盘组织和外周血中的表达

田美荣1,马玉燕1,王磊一2,范月莲1,刘振平2   

  1. 山东大学齐鲁医院  1.妇产科; 2.基因室, 济南 250012
  • 收稿日期:2009-12-26 出版日期:2010-05-16 发布日期:2010-05-16
  • 通讯作者: 马玉燕(1965- ),女,教授,主要从事围产医学的研究。 E-mail: yuyanma65@163.com
  • 作者简介:田美荣(1979- ),女,硕士研究生,主要从事围产医学的研究。

Expression of interleukin-17 in placenta and peripheralblood of patients with preeclampsia

TIAN  Meirong1, MA Yuyan1, WANG Leiyi2, FAN YueLian1, LIU Zhenping2   

  1. 1. Department of Obstetrics and Gynecology; 2. Department of Gene Laboratory,
    Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2009-12-26 Online:2010-05-16 Published:2010-05-16

摘要:

目的  探讨白细胞介素-17 (IL-17)和Th17细胞与子痫前期发病的关系。方法  选取32例重度子痫前期患者为子痫前期组,30例正常晚期妊娠孕妇为对照组。采用实时定量PCR方法检测两组胎盘组织IL-17 mRNA 表达水平;酶联免疫吸附试验双抗体夹心(ELISA)法检测外周血血清IL-17、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的浓度。结果子痫前期组胎盘IL-17 mRNA表达量较对照组升高(P<0.05);子痫前期组外周血血清IL-17、IL-6、TNF-α水平均高于对照组(P<0.01、P<0.05、P<0.01);子痫前期组外周血中IL-17与IL-6的表达水平呈正相关(r=0.402,P<0.05)。结论  IL-17和Th17细胞介导了子痫前期的发生,其过程可能与IL-6和TNF-α的参与有关。

关键词: 白细胞介素类;Th17 细胞;子痫前期;肿瘤坏死因子-α

Abstract:

Objective  To investigate the relationship of IL-17 and Th17 cells with preeclampsia. Methods  Thirty-two patients with severe preeclampsia and thirty normal pregnant women as controls were investigated. ① Expression of IL-17 mRNA in placental specimens was detected by  real-time PCR; ② Concentrations of  IL-17, IL-6 and TNFα in serum were measured by ELISA. Results  Compared with the control group,  expression of IL-17 mRNA was higher in preeclampsia placentas (P<0.05);  levels of IL-17,IL-6 and TNF-α in maternal serum were higher in the preeclampsia group than in the controls (P<0.01, P<0.05, P<0.01 respectively ); the preeclampsia group, there was a positive correlation between IL-17 and IL-6(r=0.402, P<0.05). Conclusions  IL-17 and Th17 cells may contribute to the pathogenesis and pathophysiology of preeclampsia. IL-6 and TNF-α may also be involved in the process.

Key words: Interleukins; Th17 cell; Preeclampsia; Tumor necrosis factor-α

中图分类号: 

  • R714.1
[1] 张艳慧1,马玉燕1,杨宁2,张匀2,菅凤1,王琳琳1,刘振平2. 正常妊娠不同时期胎盘组织中chemerin的表达[J]. 山东大学学报(医学版), 2013, 51(06): 81-83.
[2] 贾雪芹1,刘海英2,马玉燕2,高凌雪3,刘媛2. 肝细胞生长因子对滋养细胞HLX1基因的表达及侵袭能力的影响[J]. 山东大学学报(医学版), 2010, 48(2): 58-.
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