妊娠早期CD56brightCD16- NK细胞CD49d和CD11b的表达分析

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (5): 97-100.

妊娠早期CD56brightCD16- NK细胞CD49d和CD11b的表达分析

黄先亮1,董白桦1,胡微煦2,王庆杰2,宋丙凤2,王春娥2,刘佳2,孔北华1,曲迅2

山东大学齐鲁医院 1. 妇产科； 2. 临床基础研究所, 济南 250012

收稿日期:2010-01-20 出版日期:2010-05-16 发布日期:2010-05-16
通讯作者: 董白桦,教授,硕士生导师,主要从事生育调节的研究。 E-mail：usera9597@163.com
作者简介:黄先亮(1982- ),男,硕士研究生,主要从事生育调节的研究。
基金资助:
国家自然科学基金资助课题(30872321 30671902)；山东省自然科学基金资助课题（Y2008C02）

Exprlssions of CD49d and CD11b on CD56brightCD16- NK cell of early pregnancy

HUANG Xianliang1, DONG Baihua1, HU Weixu2, WANG Qingjie2, SONG Bingfeng2, WANG Chune2,LIU Jia2, KONG Beihua1, QU Xun2

1. Department of Gynaecology and Obstetrics; 2. Institute of Clinic and Basic Medicine Research,
Qilu Hospital of Shangdong University, Jinan 250012, China

Received:2010-01-20 Online:2010-05-16 Published:2010-05-16

摘要/Abstract

摘要：

目的探讨早期妊娠和稽留流产患者外周血CD56brightCD16- NK细胞及蜕膜组织子宫自然杀伤(uNK)细胞中整合素α4（CD49d）和αm (CD11b)的表达。方法采集18例早期妊娠妇女外周血（其中14例另取蜕膜组织）和9例稽留流产妇女外周血,Ficoll密度梯度离心法分离单个核细胞悬液,流式细胞术检测样本CD56brightCD16- NK细胞中CD49d和CD11b表达。结果 CD49d和CD11b在早期妊娠蜕膜CD56brightCD16- NK细胞表达显著低于早期妊娠妇女外周血CD56brightCD16-NK细胞(P＜0.01);稽留流产患者外周血CD56brightCD16- NK细胞的表达较早期妊娠妇女显著降低(P＜0.05)。结论 CD49d和CD11b可能在妊娠早期uNK细胞的募集中起重要作用,同时也可能参与了正常妊娠的维持。

关键词: 早期妊娠；稽留流产；uNK细胞；整合素

Abstract:

Objective To investigate the expressions of integrinα4（CD49d）and integrinαm (CD11b) on CD56brightCD16- NK cell of the peripheral blood and decidua in early pregnant women and the peripheral blood in missed abortion women. Methods Peripheral blood samples were collected from early pregnant and missed abortion female, and uNK cells were isolated from the fresh decidua of early pregnant females by mechanical trituration and Ficoll gradient centrifugation. The fluorescent intensity of the integrines was detected by FCM. Results Expressions of CD49d and CD11b on CD56brightCD16- NK cell of peripheral blood in early pregnant women those higher than were in the first trimester decidua( P＜0.01), and also higher than those in missed abortion women (P＜0.05). Conclusion CD49d and CD11b may play an important role in the recruitment of uNK cells and participates in the maintenance of normal pregnancy.

Key words: Early pregnancy; Missed abortin; uNK cell; Integrin

中图分类号:

R714.14

黄先亮1,董白桦1,胡微煦2,王庆杰2,宋丙凤2,王春娥2,刘佳2,孔北华1,曲迅2. 妊娠早期CD56brightCD16- NK细胞CD49d和CD11b的表达分析[J]. 山东大学学报(医学版), 2010, 48(5): 97-100.

HUANG Xianliang1, DONG Baihua1, HU Weixu2, WANG Qingjie2, SONG Bingfeng2, WANG Chune2,LIU Jia2, KONG Beihua1, QU Xun2. Exprlssions of CD49d and CD11b on CD56brightCD16- NK cell of early pregnancy[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(5): 97-100.

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