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山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (9): 1-4.

• 论文 •    下一篇

糖尿病大鼠学习能力与脑细胞凋亡及血糖的关系

陈青,郑冬梅,孔磊,李秋,姜秀云,管庆波,赵家军   

  1. 山东大学附属省立医院内分泌科, 济南 250021
  • 收稿日期:2009-04-12 发布日期:2009-09-16
  • 通讯作者: 赵家军(1961- ),男,主任医师,博士生导师,主要从事内 分泌代谢病的研究。Email: jjzhao@medmail.com.cn
  • 作者简介:陈青(1975- ),女,博士研究生,主治医师,主要从事糖尿病、甲状腺疾病的研究。 

Cognition dysfunction of diabetic rats and its relation to apoptosis in the frontal lobe cortex, the hippocampus and HbA1c

CHEN Qing, ZHENG Dongmei, KONG Lei, LI Qiu, JIANG Xiuyun, GUAN Qingbo, ZHAO Jiajun    

  1. Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China
  • Received:2009-04-12 Published:2009-09-16

摘要:

目的探讨糖尿病大鼠学习能力与大脑神经元凋亡状况及血糖的关系。方法采用电迷宫法测试26只糖尿病大鼠(糖尿病组)和30只正常大鼠(对照组)的学习能力,并应用原位末端标记法检测其额叶皮层和海马发生细胞凋亡的神经元数目,免疫组化法检测凋亡调节基因Bcl2、Bax的表达,并对糖尿病大鼠学习能力与额叶皮层和海马凋亡细胞数目及糖化血红蛋白进行相关分析。结果糖尿病组大鼠电迷宫测试成绩明显下降,达到学会标准前所需训练次数明显多于对照组(P<0.001),额叶皮层和海马细胞凋亡数高于对照组,Bcl2表达减少,而Bax表达则增加(P均<0.01)。糖尿病大鼠达到学会标准前所需训练次数与额叶皮层和海马细胞凋亡指数呈正相关(r=0.410,P<0.05),且与糖化血红蛋白呈正相关。结论糖尿病高血糖状态下额叶皮层与海马凋亡调节基因表达改变引起的细胞凋亡增加可能与糖尿病认知功能障碍有关。

关键词: 糖尿病, 细胞凋亡, 认知功能障碍, 大鼠,Wistar

Abstract:

To discuss the relationship among cognition function, apoptotic status in the cortex of the frontal lobe and the hippocampus and blood glucose in diabetic rats.  MethodsLearning ability of 26 diabetic rats and 30 normal rats were measured by an electric maze after 12 weeks. Then the rats were executed to obtain the brain tissues to estimate the TUNEL (terminal deoxynucleotidyl transferasemediated dUTP nickendlabeling) positive cells and expressions of Bcl2 and Bax genes in the cortex of the frontal lobe and the hippocampus. Learning ability, the number of apoptotic cells in the frontal cortex and the hippocampus, and glycosylated hemoglobin were analyzed. ResultsLearning ability of diabetic rats was worse than that of the control group, as they needed more time to reach the learning standard(P<0.001). TUNEL positive cells in the cortex of the frontal lobe and the hippocampus were more in the diabetes group than in the control group. Expression of Bcl2 gene was reduced while that of the Bax gene was increased in diabetic rats (P<0.01). The time before the diabetic rats reached the learning standard was positively correlated with the apoptotic index of the cortex of the frontal lobe and the hippocampus (r=0.410, P<0.05), and the learning ability of diabetic rats was positively correlated with HbA1c. ConclusionIncreased apoptosis induced by expression of some regulation genes may contribute to cognition dysfunction in diabetes mellitus.

Key words: Diabetes mellitus; Apoptosis; Cognition dysfunction;Rats, Wistar

中图分类号: 

  • R318.15
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