关键词: 宫颈上皮内瘤样病变, 细胞型Fas相关死亡结构域蛋白样白介素-1β转换酶抑制蛋白, 宫颈肿瘤, 胰岛素样生长因子结合蛋白-5

Abstract:

Objective〓〖WTBZ〗To investigate the expression of insulin-like growth binding protein 5 (IGFBP-5), cellular fasassociated death domain-like interleukin-1β-converting enzyme-like inhibitory protein (cFLIP), and high risk-HPV in cervical intraepithelial neoplasia（CIN）and cervical carcinoma（CC）and to evaluate their clinical significances. 〖WTHZ〗Methods〓〖WTBZ〗Immunohistochemistry (SP) was used to determine the expressions of IGFBP-5 and cFLIP proteins in 28 normal cervical tissues, 37 CIN and 40 CC tissues, RT -PCR was used to determine the mRNA, and hybrid capture Ⅱ assay was usedto determine the high-risk HPV DNA. 〖WTHZ〗Results〓〖WTBZ〗IGFBP-5 protein significantly expressed in normal tissues, CIN and CC tissues, and it was the highest in CINⅡ/Ⅲ but it was decreased in CC tissues (P<0.05). IGFBP-5 protein expression had relationships with the clinical stage, cell differentiation and lymph node metastasis. However, IGFBP-5 mRNA expression was higher in the CC group than that in the control group but was lower than that in the CINⅡ/Ⅲ groups. cFLIP expression was not found in normal cervical tissues, but was gradually increased in the CIN and CC groups with an increase of pathological degree (P<0.05). 〖WTHZ〗Conclusion〓〖WTBZ〗IGFBP-5, cFLIP and HR-HPV play potential roles in CIN and CC, and could be used to evaluate the progression and prognosis.

Key words: Insulin-like growth binding protein 5, Cellular Fas-associated death domain-like interleukin-1β-converting enzyme -like inhibitory protein,
Cervix neoplasms, Cervical intraepithelial neoplasia