电刺激对脑梗死大鼠运动功能恢复及Nogo-A、NgR表达的影响

山东大学学报(医学版)

电刺激对脑梗死大鼠运动功能恢复及Nogo-A、NgR表达的影响

杨冰，张秀清，唐吉友，宋云，孙钦建

山东大学附属千佛山医院神经内科，济南 250014

收稿日期:2008-02-26 修回日期:1900-01-01 出版日期:2008-10-16 发布日期:2008-10-16
通讯作者: 张秀清

Effects of electric stimulation on recovery of motor function and expressions of Nogo-A and Nogo receptor after acute cerebral infarction in rats

YANG Bing， ZHANG Xiu-qing， TANG Ji-you， SONG Yun， SUN Qin-jian

Department of Neurology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China

Received:2008-02-26 Revised:1900-01-01 Online:2008-10-16 Published:2008-10-16
Contact: ZHANG Xiu-qing

摘要/Abstract

摘要： 目的探讨单侧和双侧电刺激对脑梗死大鼠梗死灶周围Nogo-A、Nogo受体(NgR)表达和神经功能恢复的影响及作用机制，为电刺激在脑梗死治疗及康复中的应用提供理论依据。方法利用线栓法制作大鼠局灶性脑梗死模型，将造模成功且存活的脑梗死大鼠分为对照组、单侧电刺激组、双侧电刺激组（各32只），另外假手术组32只、正常组8只。对照组、假手术组自然恢复；单、双侧电刺激组接受电刺激治疗。于第1、3、7、14、21d利用平衡木实验检测各组大鼠神经功能情况，第3、7、14、21d利用免疫组化检测梗死灶周围皮质Nogo-A和NgR阳性蛋白的表达。结果电刺激治疗的大鼠运动功能的恢复较对照组明显改善（P＜0.05），双侧电刺激组恢复优于单侧电刺激组。正常组与假手术组各时间点Nogo-A和NgR表达差异无统计学意义（P＞0.05），脑梗死后第3、7d对照组Nogo-A的表达明显高于假手术组，电刺激后Nogo-A的表达较对照组下降（P＜0.05），双侧电刺激组较单侧电刺激组下降（P＜0.05）。脑梗死后3?d至21d对照组与假手术组NgR表达差异无统计学意义（P＞0.05），电刺激后NgR表达较对照组下降，双侧电刺激组NgR的下降较单侧电刺激组明显。结论电刺激能够促进脑梗死大鼠神经功能的恢复，双侧电刺激效果优于单侧电刺激，下调Nogo-A和NgR的表达可能是其促进运动功能恢复的机制之一。

关键词: SpragneDawley, Nogo受体, 大鼠, Nogo-A, 电刺激, 运动功能, 脑梗死

Abstract: To investigate the mechanisms of electric stimulation in improving neuro-rehabilitation by observing the effects of impaired limbs and bilateral electric stimulation on motor function and expressions of Nogo-A and Nogo receptor (NgR) following acute cerebral infarction in experimental rats. MethodsAdult male SpragueDawley (SD) rats were randomly divided into the normal (8 rats), sham operation, control, impaired limb and bilateral electric stimulation groups (each group had 32 rats). The acute cerebral infarction model was mimicked by using the middle cerebral artery occlusion (MCAO) technique. After cerebral infarction for 24h, the rats were treated with or without electric stimulation everyday according to different groups, followed by evaluation of the motor function and expression of Nogo-A and NgR in the border zone of cerebral infarction for 3, 7, 14 and 21 days by using the beam walking test (BWT) and immunohistochemistory. ResultsMotor function was significantly improved in the treatment groups with electric stimulation compared with the control group(P＜0.05). Also, the effect of the bilateral electric stimulation on recovery of motor function was better than that of the impaired limb treatment. Further studies demonstrated that the expressions of NogoA and NgR were remarkably increased in the control and treatment groups after cerebral infarction for 3 days (P＜0.05) in contrast to the sham operation group, and were decreased after 7 days and returned to the basal level following 21 days, whereas no significant point. When compared with the control group, the expressions of NogoA and NgR in the treatment groups were significantly reduced after cerebral infarction for 3 day, however, no significant differences were found after 21 days, in contrast to the impaired limb electric stimulation group, they were lower in the bilateral treatment group 7 days after cerebral infarction (P＜0.05) and returned to the same level of the impaired limb treatment group after 14 days. ConclusionsTreatment of acute cerebral infarction with electric stimulation, in a particular bilateral stimulation, can improve the movement of the paralyzed extremities and down-regulate the expressions of Nogo-A and NgR. Therefore, improvement of motor function by electric stimulation involves the inhibition of NogoA and NgR expressions after acute cerebral infarction.

Key words: Cerebral infarction, Rehabilitation, Electric stimulation, Motor function, NogoA, NgR

中图分类号:

R454.1

杨冰,张秀清,唐吉友,宋云,孙钦建. 电刺激对脑梗死大鼠运动功能恢复及Nogo-A、NgR表达的影响[J]. 山东大学学报(医学版), 2008, 46(10): 931-935.

YANG Bing,ZHANG Xiu-qing,TANG Ji-you,SONG Yun,SUN Qin-jian. Effects of electric stimulation on recovery of motor function and expressions of Nogo-A and Nogo receptor after acute cerebral infarction in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(10): 931-935.

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