蜗牛粘液来源的活性肽SK-14促进成纤维细胞的增殖和迁移

doi:10.6040/j.issn.1671-7554.0.2025.0529

摘要/Abstract

摘要： 目的基于蜗牛粘液的分离提取、酶解和序列测定,筛选并人工合成活性肽序列,评价其对成纤维细胞增殖、迁移能力的影响。方法提取蜗牛粘液蛋白并用胰蛋白酶消化4 h,液相色谱-质谱结合生物信息学分析,筛选具有促进增殖的肽段序列,通过固相合成法制备。经培养人外膜成纤维细胞(human adventitial fibroblasts, HAFs)并用不同浓度的活性肽处理,评估其对细胞增殖、迁移能力的影响。结果合成的肽序列为SK-14(N端序列:SSTIFPPTSGGAAK),分子量为1 268 Da。 SK-14能显著促进成纤维细胞增殖(P<0.001)。细胞增殖能力随SK-14质量浓度增加而呈现浓度依赖性增强,增殖能力随SK-14质量浓度升高而显著提高,并在500 μg/mL达平台期。SK-14同样可显著促进HAFs的迁移能力(P<0.001),其作用效果随着SK-14质量浓度递增上升,以1 000 μg/mL的作用最明显。结论蜗牛粘液来源的活性肽序列SK-14可促进人成纤维细胞的增殖、迁移能力,可能是蜗牛粘液参与促进伤口愈合的活性成分之一。

关键词: 蜗牛粘液, 活性肽, 成纤维细胞, 增殖, 迁移

Abstract: Objective To screen and synthesize the active peptide sequence, and to evaluate its effects on the proliferation and migration of fibroblasts based on the isolation, enzymatic digestion and sequencing of snail mucus. Methods Snail mucus protein was extracted and digested with trypsin for 4 h. The peptide sequence with proliferation promotion was screened by liquid chromatography-mass spectrometry and bioinformatics analysis, and then prepared by solid phase synthesis. Human adventitial fibroblasts(HAFs)were cultured and treated with different concentrations of active peptides to evaluate their effects on cell proliferation and migration. Results The synthesized peptide sequence was SK-14(N-terminal sequence: SSTIFPPTSGGAAK), with a molecular weight of 1,268 Da. Compared with the control group, SK-14 could significantly promote fibroblast proliferation at different concentrations(P<0.001). The cell proliferation ability increased in a concentration-dependent manner with the increase of SK-14 concentration. Within the test concentration range, the proliferation ability significantly increased with the increase of drug SK-14 concentration, and reached the plateau at 500 μg/mL. SK-14 can also significantly promote the migration ability of HAFs(P<0.001), and its effect increased with the increase of SK-14 concentration, with the most obvious effect at 1,000 μg/mL in the test concentration range. Conclusion The active peptide SK-14 derived from snail mucus can promote the proliferation and migration of HAFs, which may be one of the active components of snail mucus in promoting wound healing.

Key words: Snail mucus, Active peptides, Fibroblasts, Proliferation, Migration

中图分类号:

R654.4

李观强,施昱诚,朱可涵,胡波,黄献琛,孙元,李笃信,张喜成. 蜗牛粘液来源的活性肽SK-14促进成纤维细胞的增殖和迁移[J]. 山东大学学报 (医学版), 2025, 63(11): 1-7.

LI Guanqiang, SHI Yucheng, ZHU Kehan, HU Bo, HUANG Xianchen, SUN Yuan, LI Duxin, ZHANG Xicheng. Active peptide SK-14 derived from snail mucus promotes fibroblast proliferation and migration[J]. Journal of Shandong University (Health Sciences), 2025, 63(11): 1-7.

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