山东大学学报 (医学版) ›› 2024, Vol. 62 ›› Issue (6): 9-16.doi: 10.6040/j.issn.1671-7554.0.2023.1040
• 基础医学 • 上一篇
郭姝画1,2,樊扬3,田风1,2,王传新1,2,杜鲁涛1,2,李培龙1,2,郭兴1,4,徐硕1,4
GUO Shuhua1,2, FAN Yang3, TIAN Feng1,2, WANG Chuanxin1,2, DU Lutao1,2, LI Peilong1,2, GUO Xing1,4, XU Shuo1,4
摘要: 目的 探讨微原纤维相关蛋白3(microfibril-associated protein 3, MFAP3)在胶质母细胞瘤(glioblastoma, GBM)中的表达与临床意义,以及对于胶质瘤干细胞(glioma stem cells, GSCs)恶性生物学行为的影响。 方法 利用TCGA数据和CGGA数据分析GBM中MFAP3表达情况及其与患者预后相关性;使用Western blotting检测胶质瘤干细胞系中MFAP3蛋白表达水平;探究敲减及过表达MFAP3对胶质瘤干细胞自我更新能力和表型的影响。 结果 MFAP3在GBM中高表达,且与患者不良预后相关。间充质型GSCs中MFAP3的表达水平高于前神经元型GSCs。MFAP3敲减后,GSCs自我更新能力减弱,间充质表型标志物CD44、YKL40蛋白水平均降低,过表达与之相反。 结论 MFAP3对GBM恶性生物学进展具有重要调控作用,有望成为新的GBM临床诊断标志物和治疗干预靶点。
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