山东大学学报 (医学版) ›› 2024, Vol. 62 ›› Issue (2): 29-35.doi: 10.6040/j.issn.1671-7554.0.2023.0893
• 基础医学 • 上一篇
田欣鑫1*,王立俊1*,李琳1,孙正达2,刘海燕1
TIAN Xinxin1*, WANG Lijun1*, LI Lin1, SUN Zhengda2, LIU Haiyan1
摘要: 目的 探讨CD300c-Ig对胶原诱导性关节炎(collagen induced arthritish, CIA)小鼠关节损伤的影响及作用机制。 方法 分别在第1天及第21天免疫DBA/1J 小鼠,构建CIA模型,随机分为处理组和对照组,每组10只。处理组腹腔注射20 μg CD300c-Ig 100 μL,1次/3 d,共6次,总剂量为120 μg;对照组则腹腔注射相同质量的对照Ig蛋白(control-Ig),1次/3 d,共6次。观察四肢关节肿胀程度,记录关节炎指数评分;DBA/1J小鼠初次免疫40 d后,取小鼠脾脏制成单细胞悬液,流式细胞术检测调节性T细胞(T regulatory cells, Treg)、辅助性T细胞(T helper cells, Th17)、肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、干扰素-γ( interferon-γ, IFN-γ)的表达;取小鼠下肢,脱钙,制成切片进行苏木精-伊红染色及番红O染色,观察小鼠关节病理特征并进行评分。采用荧光定量PCR法检测叉头框蛋白P3(forkhead box protein P3, Foxp3)、白介素-17(interleukin-17, IL-17)和白介素-23(interleukin-23, IL-23)的mRNA表达。 结果 处理组关节肿胀程度较对照组减轻,关节炎指数与对照组相比明显降低;苏木精-伊红染色及番红O染色显示,处理组关节滑膜细胞增殖较对照组减轻、血管翳形成、骨质侵蚀情况均比对照组有明显改善(P<0.05);流式细胞术检测结果显示,处理组脾脏Th17细胞比例较对照组明显降低(P<0.05),Treg细胞比例较对照组明显升高(P<0.05);同时,处理组脾脏TNF-α、IFN-γ表达与对照组相比明显降低(P<0.05);荧光定量PCR结果显示,处理组IL-17和IL-23 mRNA表达水平明显低于对照组。 结论 CD300c-Ig可减轻CIA小鼠的关节损伤,其机制与其对Th17/Treg 细胞平衡的调控和抑制炎性因子的释放有关。
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