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山东大学学报 (医学版) ›› 2020, Vol. 58 ›› Issue (2): 1-6.doi: 10.6040/j.issn.1671-7554.0.2019.1047

• 基础医学 •    下一篇

6-姜酚减轻血管紧张素Ⅱ诱导的大鼠心房纤维化

赵位昆1,吕祥威2,武琦3,鲁攀1,彭丽1,覃秋语1   

  1. 1. 桂林医学院附属医院综合科(医疗保健病区), 广西 桂林 541001;2. 桂林医学院附属医院心血管内科二病区, 广西 桂林 541001;3. 徐州医科大学生理学教研室, 江苏 徐州 221004
  • 出版日期:2020-02-10 发布日期:2022-09-27
  • 通讯作者: 吕祥威. E-mail:lvxiangwei910@163.com
  • 基金资助:
    广西自然科学基金(2018GXNSFAA294096,2018GXNSFDA281039);广西高校中青年教师科研基础能力提升项目(2019KY0532);桂林医学院中青年教职工科研能力提升项目(2018glmcy048)

6-Gingerol reduces atrial fibrosis induced by angiotensin Ⅱ in rats

ZHAO Weikun1, LYU Xiangwei2, WU Qi3, LU Pan1, PENG Li1, QIN Qiuyu1   

  1. 1. Health Care Ward, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi, China;
    2. The Second Ward in Cardiovascular Department, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi, China;
    3. Department of Physiology, Xuzhou Medicine University, Xuzhou 221004, Jiangsu, China
  • Online:2020-02-10 Published:2022-09-27

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摘要： 目的 探讨6-姜酚(6-G)对血管紧张素Ⅱ(AngⅡ)诱导大鼠心房纤维化的作用及机制。 方法 8周龄雄性SD大鼠18只,按照随机数字表法分为假手术组、模型组和6-G干预+AngⅡ组,每组6只。假手术组经尾静脉注射0.9%氯化钠注射液,2 mg/kg;模型组经尾静脉注射AngⅡ,2 mg/kg,制备心房纤维化模型;6-G干预+AngⅡ组经尾静脉注射6-G,6 mg/kg,30 min后再经尾静脉注射AngⅡ,2 mg/kg。3组持续给药14 d,1次/d。各组经超声心动图检测大鼠心功能,采用ELISA法检测心肌损伤标记物的变化,采用Masson三色染色法检测大鼠心房纤维化程度,采用Western blotting法检测大鼠心房转化生长因子-β1(TGF-β1)、Ⅰ型胶原(Collagen-Ⅰ)的表达。 结果 假手术组、模型组和6-G干预+AngⅡ组3组之间相比,左心室射血分数(LVEF)(F=1.500,P=0.255)、左心室缩短率(LVFS)(F=0.793,P=0.471)、左室收缩末期内径(LVESD)(F=0.104,P=0.902)、左室舒张末期内径(LVEDd)(P=0.566)和CK-MB(F=0.089,P=0.915)、cTnT(F=0.181,P=0.836)差异无统计学意义(P>0.05)。与假手术组相比,模型组和6-G干预+AngⅡ组心房纤维化程度增加,胶原分数(F=322.527)、TGF-β1(F=44.537)、Collagen-Ⅰ(F=45.626)表达均升高,3组之间差异有统计学意义(P<0.05)。与假手术组相比,模型组大鼠上述心功能和心肌损伤标志物无明显差异(P>0.017),心房纤维化程度增加,胶原分数、TGF-β1、Collagen-Ⅰ表达均升高,差异有统计学意义(P<0.017)。与模型组相比,6-G干预+AngⅡ组大鼠上述心功能和心肌损伤标志物差异无统计学意义(P>0.017),心房纤维化程度减轻,胶原分数、TGF-β1、Collagen-Ⅰ表达均降低,差异有统计学意义(P<0.017)。 结论 6-G可减轻AngⅡ诱导的大鼠心房纤维化,其机制可能与抑制TGF-β1的表达,减少Collagen-Ⅰ的生成有关。

关键词: 6-姜酚, 血管紧张素Ⅱ, 心房纤维化, 转化生长因子-β1, Ⅰ型胶原

Abstract: Objective To investigate the effect and mechanism of 6-Gingerol(6-G)on atrial fibrosis induced by angiotensin Ⅱ(Ang II)in rats. Methods Eighteen 8-week-old male SD rats were randomly divided into sham operation group, model group, and 6-G intervention + Ang II group, with 6 rats in each group. Rats in sham operation group received 2 mg/kg 0.9% sodium chloride injection in the tail vein. Rats in model group received 2 mg/kg AngⅡ injection 山 东 大 学 学 报 (医 学 版)58卷2期 -赵位昆,等.6-姜酚减轻血管紧张素Ⅱ诱导的大鼠心房纤维化 \=-to induce atrial fibrosis. Rats in 6-G intervention + AngⅡ group received 6 mg/kg 6-G injection, and then 2 mg/kg Ang II injection after 30 min. Drugs were continuously administered for 14 days, once a day. The cardiac function was observed with echocardiography, changes of myocardial injury markers were determined with ELISA, degree of atrial fibrosis was measured with Massons trichrome staining, and TGF-β1 and collagen-Ⅰ expressions were detected with Western blotting. Results There were no statistically significant differences in LVEF(F=1.500, P=0.255), LVFS(F=0.793, P=0.471), LVESD(F=0.104, P=0.902), LVEDd(P=0.566)CK-MB(F=0.089, P=0.915), and cTnT(F=0.181, P=0.836)among the three groups(P>0.05). Compared with sham operation group, the model group and 6-G intervention + Ang II group had severer atrial fibrosis, elevated collagen fraction(F=322.527), TGF-β1(F=44.537)and Collagen-Ⅰ (F=45.626), and the differences were statistically significant(P<0.05). Compared with sham operation group, the model group had comparable cardiac function and myocardial injury markers(P>0.017), but severer atrial fibrosis, elevated collagen fraction, TGF-β1 and Collagen-I, and the differences were statistically significant(P<0.017). Compared with the model group, the 6-G intervention + AngⅡ group had no statistically different cardiac function and myocardial injury markers(P>0.017), but reduced atrial fibrosis, decreased collagen fraction, TGF-β1 and Collagen-Ⅰ, and the differences were statistically significant(P<0.017). Conclusion 6-G can improve AngⅡ-induced atrial fibrosis by inhibiting TGF-β1 and Collagen-Ⅰ expressions.

Key words: 6-Gingerol, Angiotensin Ⅱ, Atrial Fibrosis, Transforming growth factor β1, Collagen type-Ⅰ

中图分类号: 

  • R542.2
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