血清lncRNA UCA1在食管鳞癌的表达及临床意义

doi:10.6040/j.issn.1671-7554.0.2018.178

摘要/Abstract

摘要： 目的探讨长链非编码RNA(lncRNA)尿路上皮癌胚抗原1(UCA1)在食管鳞癌患者血清中的表达及潜在的临床意义。方法收集96例食管鳞癌患者血清样本,同时选取96例年龄、性别匹配的健康体检者作为健康对照组,其中12例食管鳞癌患者行术前、术后比较。采用实时荧光定量PCR的方法检测 lncRNA UCA1在食管鳞癌血清中的表达,并对食管鳞癌患者术前、术后进行比较。分析食管鳞癌患者血清lncRNA UCA1与其临床病理参数的关系,采用受试者工作曲线(ROC)对血清lncRNA UCA1的诊断效能进行评价。结果与健康对照组比较,食管鳞癌患者血清中lncRNA UCA1的表达升高,且患者术后较术前表达下降,差异有统计学意义(P<0.001)。血清lncRNA UCA1诊断食管鳞癌的ROC曲线下面积(AUC)为0.816,灵敏度和特异度分别为77.1%和76.0%。此外,与传统肿瘤标志物鳞状细胞癌相关抗原(AUC=0.679)比较,lncRNA UCA1对食管鳞癌诊断效能更高(P<0.001)。结论血清lncRNA UCA1可作为食管鳞癌早期诊断及术后监测潜在的新型肿瘤标志物。

关键词: 食管鳞癌, 长链非编码RNA UCA1, 诊断, 监测, 标志物

Abstract: Objective To explore the expression of serum long non-coding RNA(lncRNA)urothelialcarcinoma associated 1(UCA1)in esophageal squamous cell carcinoma(ESCC)and its clinical significance. Methods A total of 96 ESCC cases and another 96 age-and gender-matched healthy controls were involved. The serum samples were collected to determine the expression of lncRNA UCA1 with qRT-PCR. The lncRNA UCA1 expression of 12 ESCC patients were compared before and after operation. The correlation between UCA1 expression and clinical pathological parameters of ESCC were analyzed, and the diagnostic value of serum lncRNA UCA1 was evaluated with the receiver operating characteristic curve(ROC). Results Compared with controls, ESCC patients had upregulated lncRNA UCA1 expression, which decreased after operation(P<0.001). In addition, the area under the curve(AUC)was 0.816, providing a sensitivity of 77.1% and a specificity of 76.0%. Moreover, lncRNA UCA1 was statistically superior to squamous cell carcinoma antigen(AUC=0.679)in discriminating ESCC patients from healthy controls(P<0.001). Conclusion Serum lncRNA UCA1 may serve as a potential tumor biomarker for the early diagnosis and postoperative monitoring of ESCC.

Key words: Esophageal squamous cell carcinoma, Long non-coding RNA UCA1, Diagnosis, Monitoring, Biomarker

中图分类号:

R574

闫素真,杜鲁涛,王丽丽,王传新. 血清lncRNA UCA1在食管鳞癌的表达及临床意义[J]. 山东大学学报 (医学版), 2018, 56(6): 41-46.

YAN Suzhen, DU Lutao, WANG Lili, WANG Chuanxin. Expression and clinical significance of serum lncRNA UCA1 in esophageal squamous cell carcinoma[J]. Journal of Shandong University (Health Sciences), 2018, 56(6): 41-46.

参考文献

[1] Torre LA, Bray F, Siegel RL, et al. Global cancer statistics, 2012[J]. CA Cancer J Clin, 2015, 65(2): 87-108.
[2] Sakai NS, Samia-Aly E, Barbera M, et al. A review of the current understanding and clinical utility of miRNAs in esophageal cancer[J]. Semin Cancer Biol, 2013, 23(6 Pt B): 512-521.
[3] Liao Y, Xing S, Xu B, et al. Evaluation of the circulating level of fibroblast activation protein alpha for diagnosis of esophageal squamous cell carcinoma[J]. Oncotarget, 2017, 8(18): 30050-30062.
[4] Quinodoz S, Guttman M. Long noncoding RNAs: an emerging link between gene regulation and nuclear organization[J]. Trends Cell Biol, 2014, 24(11): 651-663.
[5] Deniz E, Erman B. Long noncoding RNA(lincRNA), a new paradigm in gene expression control[J]. Funct Integr Genomics, 2017, 17(2-3): 135-143.
[6] Tong YS, Wang XW, Zhou XL, et al. Identification of the long non-coding RNA POU3F3 in plasma as a novel biomarker for diagnosis of esophageal squamous cell carcinoma[J]. Mol Cancer, 2015, 14: 3. doi: 10.1186/1476-4598-14-3.
[7] Hu HB, Jie HY, Zheng XX. Three circulating lncRNA predict early progress of esophageal squamous cell carcinoma[J]. Cell Physiol Biochem, 2016, 40(1-2):117-125.
[8] Li JY, Ma X, Zhang CB. Overexpression of long non-coding RNA UCA1 predicts a poor prognosis in patients with esophageal squamous cell carcinoma[J]. Int J Clin Exp Pathol, 2014, 7(11): 7938-7944.
[9] Xia H, Ma L, Li J, et al. Elevated HBXIP expressionis associated with aggressive phenotype and poor prognosis in esophageal squamous cell carcinoma[J]. Am J Cancer Res, 2017, 7(11): 2190-2198.
[10] Wang KC, Chang HY. Molecular mechanisms of long noncoding RNAs[J]. Mol Cell, 2011, 43(6): 904-914.
[11] Yuan JH, Liu XN, Wang TT, et al. The MBNL3 splicing factor promotes hepatocellular carcinoma by increasing PXN expression through the alternative splicing of lncRNA-PXN-AS1[J]. Nat Cell Biol, 2017, 19(7): 820-832.
[12] Wang XS, Zhang Z, Wang HC, et al. Rapid identification of UCA1 as a very sensitive and specific unique marker for human bladder carcinoma[J]. Clin Cancer Res, 2006, 12(16): 4851-4858.
[13] Wang Y, Chen W, Yang C, et al. Long non-coding RNA UCA1a(CUDR)promotes proliferation and tumorigenesis of bladder cancer[J]. Int J Oncol, 2012, 41(1): 276-284.
[14] Zhou Y, Wang X, Zhang J, et al. Artesunate suppresses the viability and mobility of prostate cancer cells through UCA1, the sponge of miR-184[J]. Oncotarget, 2017, 8(11): 18260-18270.
[15] Liu H, Wang G, Yang L, et al. Knockdown of long non-coding RNA UCA1 increases the tamoxifen sensitivity of breast cancer cells through inhibition of Wnt/beta-catenin pathway[J]. PLoS One, 2016, 11(12): e0168406. doi: 10.1371/journal.pone.0168406.
[16] Chen P, Wan D, Zheng D, et al. Long non-coding RNA UCA1 promotes the tumorigenesis in pancreatic cancer[J]. Biomed Pharmacother, 2016, 83: 1220-1226. doi: 10.1016/j.biopha.2016.08.041.
[17] Zuo ZK, Gong Y, Chen XH, et al. TGFbeta1-induced lncRNAUCA1 upregulation promotes gastric cancer invasion and migration[J]. DNA Cell Biol, 2017, 36(2): 159-167.
[18] Bian Z, Jin L, Zhang J, et al. LncRNA-UCA1 enhances cell proliferation and 5-fluorouracil resistance in colorectal cancer by inhibiting miR-204-5p[J]. Sci Rep, 2016, 6: 23892. doi: 10.1038/srep23892.
[19] Xiao JN, Yan TH, Yu RM, et al. Long non-coding RNA UCA1 regulates the expression of Snail2 by miR-203 to promote hepatocellular carcinoma progression[J]. J Cancer Res Clin Oncol, 2017, 143(6): 981-990.
[20] 江楠, 田海华, 潘金昌, 等. 循环长链非编码RNA作为生物标志物在肿瘤分子诊断中应用[J].生物工程学报, 2017, 33(6): 910-922. JIANG Nan, TIAN Haihua, PAN Jinchang, et al. Circulating long noncoding RNAs as biomarkers in tumor diagnosis[J]. Chinese Journal of Biotechnology, 2017, 33(6): 910-922.
[21] 方海云, 高丰厚. 循环长链非编码RNA在消化道肿瘤中的研究进展[J].现代肿瘤医学, 2017, 25(13): 2160-2163. FANG Haiyun, GAO Fenghou. Research progress of long chain non coding RNA in digestive tract tumor[J]. Modern Oncology, 2017, 25(13): 2160-2163.
[22] Duan W, Du L, Jiang X, et al. Identification of a serum circulating lncRNA panel for the diagnosis and recurrence prediction of bladder cancer[J]. Oncotarget, 2016, 7(48): 78850-78858.
[23] Wang W, He X, Zheng Z, et al. Serum HOTAIR as a novel diagnostic biomarker for esophageal squamous cell carcinoma[J]. Mol Cancer, 2017, 16(1): 75. doi: 10.1186/s12943-017-0643-6.
[24] Zheng ZK, Pang C, Yang Y, et al. Serum long noncoding RNA urothelial carcinoma-associated 1: a novel biomarker for diagnosis and prognosis of hepatocellular carcinoma[J]. J Int Med Res, 2018, 46(1): 348-356.
[25] Wen JJ, Ma YD, Yang GS, et al. Analysis of circulating long non-coding RNA UCA1 as potential biomarkers for diagnosis and prognosis of osteosarcoma[J]. Eur Rev Med Pharmacol Sci, 2017, 21(3): 498-503.

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