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山东大学学报 (医学版) ›› 2018, Vol. 56 ›› Issue (10): 86-92.doi: 10.6040/j.issn.1671-7554.0.2017.925

• • 上一篇    

黏蛋白1C亚基对食管癌细胞生物学行为的影响

公孙鑫,辛钟伟,史墨,秦启明,姜斌,宋学敏,刘相燕   

  1. 山东大学附属省立医院胸外科, 山东 济南 250021
  • 收稿日期:2017-09-26 发布日期:2022-09-27
  • 通讯作者: 刘相燕. E-mail:liuxy6638@126.com
  • 基金资助:
    山东省自然科学基金(ZR2014HM013)

Effects of mucin1-C on the biological behaviors of esophageal squamous cell carcinoma cells

GONGSUN Xin, XIN Zhongwei, SHI Mo, QIN Qiming, JIANG Bin, SONG Xuemin, LIU Xiangyan   

  1. Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2017-09-26 Published:2022-09-27

摘要: 目的 探讨黏蛋白1 C亚基(MUC1-C)在食管鳞癌中的表达定位及在肿瘤细胞恶性生物学行为中的作用。 方法 Western blotting检测MUC1-C在食管鳞癌组织及正常组织细胞核内的表达;运用穿膜肽Go-201抑制MUC1-C入核后,集落实验及CCK-8实验检测食管鳞癌细胞的活性、生长和增殖能力;划痕实验及Transwell小室实验检测食管鳞癌细胞迁移及侵袭能力;流式细胞术检测食管鳞癌细胞凋亡率。 结果 MUC1-C在食管鳞癌组织细胞核内的表达高于正常组织,抑制MUC1-C入核后食管鳞癌细胞活性、生长、增殖、迁移、侵袭能力均降低,凋亡率增高。 结论 MUC1-C通过入核发挥作用,能诱导和促进食管鳞癌细胞的恶性生长、增殖、迁移、侵袭,抑制肿瘤细胞的凋亡。

关键词: 食管鳞癌, 黏蛋白1C亚基, 转移, 凋亡

Abstract: Objective To explore the expression of mucin1-C(MUC1-C)in esophageal squamous cell carcinoma(ESCC)and its role in the malignant biological behaviors of ESCC cells. Methods The expressions of MUC1-C in the ESCC tissues and normal tissues were detected with Western blotting. After MUC1-C entrance into the nucleus was inhibited with Go-201, the activity, growth and proliferation of ESCC cells were assessed with colony-forming assay and CCK-8 test; the cell migration and invasion abilities were measured with wound healing and Transwell assay, respectively; the apoptosis rate was determined with flow cytometry. Results The expression of MUC1-C in ESCC tissues was higher than that in normal tissues. The activity, growth, proliferation, migration and invasion of ESCC cells decreased while the apoptosis rate increased after MUC1-C entrance into the nucleus was inhibited with Go-201. Conclusion MUC1-C can promote the growth, proliferation, migration and invasion while inhibit the apoptosis of ESCC cells.

Key words: Esophageal squamous cell carcinoma, Mucin1-c, Migration, Apoptosis

中图分类号: 

  • R574
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