黏蛋白1C亚基对食管癌细胞生物学行为的影响

doi:10.6040/j.issn.1671-7554.0.2017.925

摘要/Abstract

摘要： 目的探讨黏蛋白1 C亚基(MUC1-C)在食管鳞癌中的表达定位及在肿瘤细胞恶性生物学行为中的作用。方法 Western blotting检测MUC1-C在食管鳞癌组织及正常组织细胞核内的表达;运用穿膜肽Go-201抑制MUC1-C入核后,集落实验及CCK-8实验检测食管鳞癌细胞的活性、生长和增殖能力;划痕实验及Transwell小室实验检测食管鳞癌细胞迁移及侵袭能力;流式细胞术检测食管鳞癌细胞凋亡率。结果 MUC1-C在食管鳞癌组织细胞核内的表达高于正常组织,抑制MUC1-C入核后食管鳞癌细胞活性、生长、增殖、迁移、侵袭能力均降低,凋亡率增高。结论 MUC1-C通过入核发挥作用,能诱导和促进食管鳞癌细胞的恶性生长、增殖、迁移、侵袭,抑制肿瘤细胞的凋亡。

关键词: 食管鳞癌, 黏蛋白1C亚基, 转移, 凋亡

Abstract: Objective To explore the expression of mucin1-C(MUC1-C)in esophageal squamous cell carcinoma(ESCC)and its role in the malignant biological behaviors of ESCC cells. Methods The expressions of MUC1-C in the ESCC tissues and normal tissues were detected with Western blotting. After MUC1-C entrance into the nucleus was inhibited with Go-201, the activity, growth and proliferation of ESCC cells were assessed with colony-forming assay and CCK-8 test; the cell migration and invasion abilities were measured with wound healing and Transwell assay, respectively; the apoptosis rate was determined with flow cytometry. Results The expression of MUC1-C in ESCC tissues was higher than that in normal tissues. The activity, growth, proliferation, migration and invasion of ESCC cells decreased while the apoptosis rate increased after MUC1-C entrance into the nucleus was inhibited with Go-201. Conclusion MUC1-C can promote the growth, proliferation, migration and invasion while inhibit the apoptosis of ESCC cells.

Key words: Esophageal squamous cell carcinoma, Mucin1-c, Migration, Apoptosis

中图分类号:

R574

公孙鑫,辛钟伟,史墨,秦启明,姜斌,宋学敏,刘相燕. 黏蛋白1C亚基对食管癌细胞生物学行为的影响[J]. 山东大学学报 (医学版), 2018, 56(10): 86-92.

GONGSUN Xin, XIN Zhongwei, SHI Mo, QIN Qiming, JIANG Bin, SONG Xuemin, LIU Xiangyan. Effects of mucin1-C on the biological behaviors of esophageal squamous cell carcinoma cells[J]. Journal of Shandong University (Health Sciences), 2018, 56(10): 86-92.

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