交感神经信号对大鼠正畸牙根吸收的影响

doi:10.6040/j.issn.1671-7554.0.2017.580

摘要/Abstract

摘要： 目的探讨交感神经信号对大鼠正畸牙移动中牙根吸收的影响。方法选取36只8周龄无特定病原体(SPF)级健康雄性SD大鼠建立正畸牙移动实验模型,随机分为普萘洛尔(PRO)组、异丙肾上腺素(ISO)组和对照组,每组12只。各组大鼠分别腹腔注射PRO［20 mg/(kg·d)］、ISO［5 mg/(kg·d)］和等体积的生理盐水。镍钛螺旋拉簧每周加力1次,3组动物于正畸加力14 d后处死。分离大鼠上颌骨及第一磨牙,采用苏木精-伊红(HE)染色光镜观察各组大鼠牙根吸收情况,并用MicroCT测量分析第一磨牙近中根的体积,比较各组之间的差异。应用SPSS 19.0对数据进行统计学分析。结果 HE染色显示PRO组的牙根表面吸收陷窝面积和深度与其他两组相比表现最轻,ISO组的牙根吸收程度较其他两组严重,可达牙本质。MicroCT结果显示PRO组的上颌第一磨牙近中根平均牙根体积明显大于对照组(P<0.01),ISO组的平均牙根体积小于对照组(P<0.05)。结论交感神经信号对正畸过程中根吸收有影响,神经激活会促进牙根吸收,神经抑制会减少牙根吸收。

关键词: 交感神经, 根吸收, MicroCT, 正畸, 大鼠

Abstract: Objective To explore the effects of sympathetic signals on root resorption in rats during orthodontic tooth movement. Methods Models of orthodontic tooth movement were established in 36 male 8-week old specific pathogen free(SPF)SD rats, which were randomly divided into 3 groups: propranolol(PRO)group, isoprenaline(ISO)group, and control group. The rats in the 3 groups were intraperitoneally injected with PRO［20 mg/(kg·d)］, ISO［5 mg/(kg·d)］ and isometric 0.9% saline, respectively, and were executed with force once every 7 days. After 14 days, the rats were sacrificed. The maxillae and first molars were separated. The root resorption was observed with hematoxylin-eosin(HE)staining.The volume of the mesial root of the first molars were measured with MicroCT. The differences among the 3 groups were compared using the SPSS 19.0 software. Results The results of HE staining showed that root resorption pits appeared in all 3 groups. The depth and scope of root resorption pits were the most serious in the ISO group, followed by the control and PRO groups. The results of MicroCT showed that the volume of mesial root of the first molars in the PRO group was larger than that in the control group(P<0.01), while the volume in the ISO group was obviously smaller than that in the control group(P<0.05). Conclusion Sympathetic signals can influence the root resorption during orthodontic tooth movement. The activation of signals promotes root resorption while the suppression of signals inhibits resorption.

Key words: Sympathetic nerve, Root resorption, MicroCT, Orthodontics, Rats

中图分类号:

R783.5

汪倩倩,王旭霞,乔青芳,王英姿,杨盼盼,张君. 交感神经信号对大鼠正畸牙根吸收的影响[J]. 山东大学学报 (医学版), 2018, 56(1): 76-80.

WANG Qianqian, WANG Xuxia, QIAO Qingfang, WANG Yingzi, YANG Panpan, ZHANG Jun. Effects of sympathetic signals on orthodontic root resorption in rats[J]. Journal of Shandong University (Health Sciences), 2018, 56(1): 76-80.

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