山东大学学报 (医学版) ›› 2018, Vol. 56 ›› Issue (1): 38-44.doi: 10.6040/j.issn.1671-7554.0.2017.1038
• • 上一篇
李弋,彭静,张蕾,陆劲松
LI Yi, PENG Jing, ZHANG Lei, LU Jingsong
摘要: 目的 观察异甘草酸镁对乳腺癌生物学行为的影响,并初步探讨其作用机制。 方法 将三阴性乳腺癌细胞株MDA-MB-231与ER阳性乳腺癌细胞株MCF-7用不同浓度(0、50、500 μmol/L)异甘草酸镁处理,分别采用CCK-8法、划痕实验、LDH活性检测观察异甘草酸镁对两种细胞株细胞增殖、迁移、死亡的作用;利用Western blotting检测异甘草酸镁对细胞不同凋亡蛋白(Bax、 Bcl-2、 Caspase-3、 Caspase-9、HSP70与HSP90)的表达。 结果 不同浓度异甘草酸镁处理后,乳腺癌细胞的增殖、迁移能力被明显抑制,细胞自噬、凋亡增加;与化疗药物顺铂联合使用后,异甘草酸镁能够提高顺铂对乳腺癌细胞迁移能力的抑制作用。在MDA-MB-231细胞株中,异甘草酸镁能够降低HSP90蛋白的表达,促进Bax蛋白的表达;在MCF-7细胞株中,异甘草酸镁能够降低HSP70蛋白的表达。 结论 异甘草酸镁对乳腺癌细胞株的生物学行为存在一定抑制作用,与化疗药物顺铂联合使用,能够提高顺铂对肿瘤细胞的抑制作用。异甘草酸镁在MCF-7及MDA-MB-231细胞株中的作用机制存在一定差异。
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