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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (12): 57-61.doi: 10.6040/j.issn.1671-7554.0.2015.901

• 临床医学 • 上一篇    下一篇

可溶性晚期糖基化终末产物受体及其基因多态性与2型糖尿病的易感性分析

李方   

  1. 山东大学附属济南市中心医院保健科, 山东济南 250014
  • 收稿日期:2015-09-23 出版日期:2015-12-10 发布日期:2015-12-10
  • 通讯作者: 李方。E-mail:mbfb@sdu.edu.cn E-mail:mbfb@sdu.edu.cn

Association between receptor of advanced glycation end products gene polymorphisms, soluble receptor of advanced glycation end products and susceptibility of type 2 diabetes mellitus

LI Fang   

  1. Department of Preventive Medicine, Jinan Central Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2015-09-23 Online:2015-12-10 Published:2015-12-10

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摘要： 目的 探讨可溶性晚期糖基化终末产物受体(sRAGE)水平及晚期糖基化终末产物受体(RAGE)基因单核苷酸位点多态性与2型糖尿病的关联。方法 选择184例2型糖尿病患者作为糖尿病组,190例健康体检者作为正常对照组。ELISA方法检测sRAGE水平,PCR-TaqMan-MGB探针检测rs2070600、rs1800624、rs1800625及rs184003基因分型。结果 糖尿病组sRAGE水平[M(P25,P75)]为1087.4(713.5,1213.4)pg/mL,正常对照组sRAGE为908.2(674.6,1107.1)pg/mL,两组差异有统计学意义(P<0.001)。糖尿病组与正常对照组间4个位点的最小等位基因频率分布均无统计学差异,且在基因型分析中也无阳性发现。线性相关分析表明,4个位点与sRAGE水平均无明显关联性。结论 sRAGE水平与2型糖尿病的发病相关,但RAGE位点多态性与糖尿病无明显关联。RAGE位点多态性与sRAGE水平无关联。

关键词: 可溶性晚期糖基化终末产物受体, 晚期糖基化终末产物受体, 单核苷酸基因多态, 2型糖尿病

Abstract: Objective To estimate the level of soluble receptor of advanced glycation end product (sRAGE) in type 2 diabetes mellitus, as well as its association with single nucleotide polymorphism (SNP) of receptor of advanced glycation end product (RAGE). Methods A total of 184 patients with type 2 diabetes mellitus and 190 healthy controls were recruited. ELISA was applied to test sRAGE, and PCR-TaqMan-MGB probe was used for the genotyping of rs2070600, rs1800624, rs1800625 and rs184003. Results The level of sRAGE[M(P25,P75)] in type 2 diabetes mellitus patients was[1087.4(713.5,1213.4) pg/mL], which was significantly higher than that of the controls[908.2(674.6,1107.1 pg/mL)]. As to the allele analysis, no significant differences were observed in the minor allele frequency comparisons of the 4 SNPs, and no association was found between the 4 SNPs and sRAGE level in the linear regression. Conclusion The elevated level of sRAGE is associated with type 2 diabetes mellitus, but the polymorphism of RAGE is not. There is no significant association between polymorphism of SNPs in RAGE and sRAGE level.

Key words: Single nucleotide polymorphism, Type 2 diabetes mellitus, Soluble receptor for advanced glycation end products, Receptor for advanced glycation end products

中图分类号: 

  • R589
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