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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (5): 27-30.doi: 10.6040/j.issn.1671-7554.0.2014.998

• 基础医学 • 上一篇    下一篇

血糖水平对糖尿病大鼠心肌GLUT1和GLUT4 mRNA表达的影响

韩云凤1, 徐华2, 刘骞3, 孙汉辰1, 许丹丹1, 侯为开1, 黄庆先4   

  1. 1. 山东大学齐鲁医院内分泌科, 山东 济南 250012;
    2. 济南市第五人民医院内分泌科, 山东 济南 250022;
    3. 泰安市第一人民医院内分泌科, 山东 泰安 271000;
    4. 山东大学齐鲁医院青岛院区内分泌科, 山东 青岛 260012
  • 收稿日期:2014-12-25 修回日期:2015-04-02 出版日期:2015-05-10 发布日期:2015-05-10
  • 通讯作者: 黄庆先。E-mail:hqx19860422@126.com E-mail:hqx19860422@126.com
  • 基金资助:
    国家自然科学基金(81170352)

Effect of blood glucose on expressions of GLUT1 and GLUT4 mRNA in myocardial tissues of diabetic rats

HAN Yunfeng1, XU Hua2, LIU Qian3, SUN Hanchen1, XU Dandan1, HOU Weikai1, HUANG Qingxian4   

  1. 1. Department of Endocrinology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Endocrinology, The Fifth People's Hospital of Jinan, Jinan 250022, Shandong, China;
    3. Department of Endocrinology, First People's Hospital of Taian, Taian 271000, Shandong, China;
    4. Department of Endocrinology, Qingdao Branch of Qilu Hospital, Shandong University, Qingdao 260012, Shandong, China
  • Received:2014-12-25 Revised:2015-04-02 Online:2015-05-10 Published:2015-05-10

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摘要： 目的 利用大鼠糖尿病模型探讨不同血糖水平对心肌GLUT1和GLUT4 mRNA表达的影响。方法 SD大鼠50只,随机选取10只为正常对照组(NC组),另外40只建立糖尿病模型。取建模成功糖尿病大鼠36只(DM组),随机分为糖尿病血糖未控制组(A组,血糖>16.7 mmol/L)、糖尿病血糖控制较差组(B组,血糖14～16.7 mmol/L)、糖尿病血糖控制一般组(C组,血糖10～13.9 mmol/L)和糖尿病血糖控制良好组(D组,血糖<10 mmol/L),每组9只。腹腔注射甘精胰岛素,12周后称体质量,取血测空腹血糖(FBG)、糖化血红蛋白(HbA1c),并将大鼠处死取心肌组织,采用RT-PCR法检测GLUT1和GLUT4 mRNA。结果 与NC组相比,DM组心肌组织GLUT1和GLUT4mRNA表达水平显著降低(P<0.05)。经胰岛素控制血糖后,DM组心肌组织GLUT1和GLUT4mRNA表达水平有所升高,A组、B组、C组和D组表达量依次升高,各组间差异有统计学意义(P<0.05)。DM组心肌组织GLUT1和GLUT4mRNA表达水平与血糖及HbA1c水平呈显著负相关(P<0.05)。结论 血糖长期慢性升高可使DM大鼠心肌组织GLUT1和GLUT4mRNA的表达降低,是机体对长期高血糖的一种代偿性保护。

关键词: 葡萄糖转运蛋白1, 糖化血红蛋白, 心肌组织, 葡萄糖转运蛋白4, 糖尿病

Abstract: Objective To evaluate the effect of blood glucose on expressions of GLUT1 and GLUT4 mRNA in diabetic rats. Methods A total of 50 healthy SD rats were randomly divided into normal control group (n=10, NC group), and diabetic model group (n=40, DM group). Of the 40 DM models, 36 were divided into 4 subgroups according to different levels of blood glucose: >16.7 mmol/L (A group), 14-16.7 mmol/L (B group), 10-14 mmol/L (C group), < 10 mmol/L (D group), with 9 animals in each group. Insulin glargine was injected into abdomen. Twelve weeks later, blood samples were collected to detect FBG and HbA1c. Myocardial tissues were collected to measure GLUT1 and GLUT4 mRNA with PT-PCR. Results Compared with the NC group, GLUT1 and GLUT4 mRNA expressions significantly reduced in all DM groups (P<0.05). After blood glucose level was controlled with insulinglargine, GLUT1 and GLUT4 mRNA expressions slightly escalated in all DM groups in the following order: D > C > B > A (P<0.05). The GLUT1 and GLUT4 mRNA expressions were negatively correlated with the level of glucose and HbA1c (P<0.05). Conclusion Chronic hyperglycemia down-regulates GLUT1 and GLUT4 mRNA expressions. This may be a protection against chronic high blood glucose mediated by GLUT1 and GLUT4.

Key words: Glucose transporter 4, Myocardial tissues, Hemoglobin A1c, Diabetes, Glucose transporter 1

中图分类号: 

  • R589.1
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