您的位置：山东大学 -> 科技期刊社 -> 《山东大学学报（医学版）》

山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (5): 31-35.doi: 10.6040/j.issn.1671-7554.0.2015.047

• 基础医学 • 上一篇    下一篇

病毒性心肌炎小鼠调节性B细胞的变化

岑治宏, 郭予洁, 伍伟锋, 李丽萍, 周秋曦   

  1. 广西医科大学第一附属医院心内科, 广西 南宁 530021
  • 收稿日期:2015-01-13 修回日期:2015-04-01 出版日期:2015-05-10 发布日期:2015-05-10
  • 通讯作者: 伍伟锋。E-mail:wucna65@163.com E-mail:wucna65@163.com
  • 基金资助:
    国家自然科学基金(81450055)

Changes of regulatory B cells in mice with viral myocarditis induced by Coxsackie virus

CEN Zhihong, GUO Yujie, WU Weifeng, LI Liping, ZHOU Qiuxi   

  1. Department of Cardiology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China
  • Received:2015-01-13 Revised:2015-04-01 Online:2015-05-10 Published:2015-05-10

PDF (PC)

129

摘要： 目的 观察病毒性心肌炎小鼠不同时间点调节性B淋巴细胞(Breg)亚群的变化,探讨其在病毒性心肌炎发病中的作用。方法 将小鼠随机分为实验组和对照组,实验组腹腔注射柯萨奇病毒B3(CVB3),对照组注射等量磷酸盐缓冲液(PBS)。依据注射后的时间,实验组和对照组均设1周和2周时间点亚组。应用苏木素伊红染色后观察心肌病理改变并计算心肌病理积分,采用RT-PCR法检测心肌组织中CVB3 mRNA的表达水平,采用流式细胞术检测小鼠脾脏Breg细胞亚群占CD19+细胞的比例。结果 实验组心肌炎症水平及CVB3 mRNA表达水平均明显高于对照组;实验组1周亚组心肌组织病理积分低于2周亚组,但实验组1周亚组心肌CVB3 mRNA表达水平却高于2周亚组,差异均有统计学意义(P<0.05)。实验组小鼠脾Breg细胞比例均高于对照组,且实验组1周亚组小鼠脾Breg细胞比例高于2周亚组,差异有统计学意义(P<0.05)。结论 病毒性心肌炎小鼠中调节性B细胞表达增高,其可能参与病毒性心肌炎的发病过程。

关键词: 肠道病毒属, CD19+CD1dhiCD5+ B细胞, 心肌炎, 炎症, 调节性B细胞

Abstract: Objective To observe the changes of regulatory B (Breg) cells in mice with viral myocarditis (VMC) induced by coxsackie virus B3 (CVB3), and to explore the role of Breg cells in VMC mice. Methods Balb/c male mice were randomly divided into experimental group and control group. Mice in the experimental group were intraperitoneally infected with CVB3 to establish VMC models, while mice in the control group were treated with phosphate-buffered saline (PBS) intraperitoneally. According to the time after injection of CVB3 or PBS, all mice in the two groups were randomly divided into 1-week and 2-week subgroups. Myocardial histopathological scores were determined in hematoxylin eosin stained sections. Expression of CVB3 mRNA in heart was measured with real-time reverse transcription-polymerase chain reaction (RT-PCR), and the frequency of splenic Breg subsets in CD19+ B cells was measured with flow cytometry. Results There were significant differences in the pathological scores and expression of myocardial CVB3 mRNA between the VMC mice and controls. The pathological scores of 1-week experimental subgroup were lower than those of 2-week experimental subgroup, while the expression of myocardial CVB3 mRNA in 1-week experimental subgroup was higher than that in 2-week experimental subgroup (all P<0.05). Compared with controls, the frequency of splenic Breg cells in the VMC mice increased significantly at different time points. Moreover, the frequency of splenic Breg cells in 1-week experimental subgroup was higher than that in the 2-week experimental subgroup (all P<0.05). Conclusion Breg cells increase in mice with CVB3-induced VMC, indicating that Breg cells mayplay an important role in the pathogenesis of CVB3-induced VMC.

Key words: Inflammation, Myocarditis, CD19+CD1dhiCD5+ B cells, Enterovirus, Regulatory B cells

中图分类号: 

  • R542.2
[1] Caforio AL, Calabrese F, Angelini A, et al. A prospective study of biopsy-proven myocarditis: prognostic relevance of clinical and aetiopathogenetic features at diagnosis[J]. Eur Heart J, 2007, 28(11):1326-1333.
[2] Sagar S, Liu PP, Cooper LT. Myocarditis[J]. Lancet, 2012, 379(9817): 738-747.
[3] Marchant DJ, Boyd JH, Lin DC, et al. Inflammation in myocardial diseases[J]. Circ Res, 2012, 110(1):126-144.
[4] DiLillo DJ, Matsushita T, Tedder TF. B10 cells and regulatory B cells balance immune responses during inflammation, autoimmunity, and cancer[J]. Ann N Y Acad Sci, 2010, 1183(2010): 38-57.
[5] Yanaba K, Bouaziz JD, Haas KM, et al. A regulatory B cell subset with a unique CD1dhiCD5+ phenotype controls T cell-dependent inflammatory responses[J]. Immunity, 2008, 28(5):639-650.
[6] Yang M, Rui K, Wang S, et al. Regulatory B cells in autoimmune diseases[J]. Cell Mol Immunol, 2013, 10(2):122-132.
[7] 孔清, 高梦莎, 薛贻敏, 等. 白细胞介素-17通过促进巨噬细胞分泌白细胞介素-27参与小鼠病毒性心肌炎的发生[J]. 中华心血管病杂志, 2014, 42(5): 428-432. KONG Qing, GAO Mengsha, XUE Yimin, et al. Interleukin-17 contributes to the macrophage secretion of interleukin-27 in a murine model of viral myocarditis[J]. Chin J Cardiol, 2014, 42(5):428-432.
[8] Candando KM, Lykken JM, Tedder TF. B10 cell regulation of health and disease[J]. Immunol Rev, 2014, 259(1):259-272.
[9] Yanaba K, Bouaziz JD, Matsushita T, et al. The development and function of regulatory B cells expressing IL-10 (B10 cells) requires antigen receptor diversity and TLR signals[J]. J Immunol, 2009, 182(12):7459-7472.
[10] 秦瑶, 张梅, 蒋瑞妹, 等. 小鼠 B10细胞的分离、鉴定及功能特征[J]. 中华微生物学和免疫学杂志, 2013, 33(11): 819-827. QIN Yao, ZHANG Mei, JIANG Ruimei, et al. Isolation, identification and functional characteristics of murine B10 cells[J]. Chin J Microbiol Immunol, 2013, 33(11): 819-827.
[11] 伍昌林, 王前, 郑磊, 等. CITP患者外周血Breg与CD4+T细胞的相关性及临床意义[J]. 中国实验血液学杂志, 2013, 21(6): 1517-1521. WU Changlin, WANG Qian, ZHENG Lei, et al. Correlation of breg with CD4+T Cells of peripheral blood in patients with CITP and its clinical significance[J]. J Exp Hematol, 2013, 21(6): 1517-1521.
[12] Yang M, Deng J, Liu Y, et al. IL-10-producing regulatory B10 cells ameliorate collagen-induced arthritis via suppressing Th17 cell generation[J]. Am J Pathol, 2012, 180(6): 2375-2385.
[13] Matsushita T, Yanaba K, Bouaziz JD, et al. Regulatory B cells inhibit EAE initiation in mice while other B cells promote disease progression[J]. J Clin Invest, 2008, 118(10):3420-3430.
[14] Yang X, Yang J, Chu Y, et al. T follicular helper cells and regulatory B cells dynamics in systemic lupus erythematosus[J]. PLoS One, 2014, 9(2):88441.
[15] Shi L, Bi M, Yang R, et al. Defective expression of regulatory B cells in iodine-induced autoimmune thyroiditis in non-obese diabetic H-2(h4) mice[J]. J Endocrinol Invest, 2014, 37(1):43-50.
[16] Sheng JR, Quan S, Soliven B. CD1d(hi)CD5+ B Cells expanded by GM-CSF in vivo suppress experimental autoimmune myasthenia gravis[J]. J Immunol, 2014, 193(6):2669-2677.
[1] 徐宁宇 王磊 郝恩魁 苏国海. STEMI患者急诊PCI前口服阿托伐他汀对炎症介质及左心室功能的影响[J]. 山东大学学报(医学版), 2209, 47(6): 69-72.
[2] 郝跃伟 刘雪平 赵婷婷 郑敏 王一兵. 环氧化酶2基因多态性与动脉粥样硬化缺血性脑卒中的相关性[J]. 山东大学学报(医学版), 2209, 47(6): 95-98.
[3] 张栾,陈欧,栾云,朱晓波,陈元,王一彪. Gemigliptin对野百合碱诱导的肺动脉高压大鼠治疗作用及炎症因子的影响[J]. 山东大学学报（医学版）, 2017, 55(5): 19-22.
[4] 闫松鹤,高立芬,赵伟,王燕. 人牙根尖乳头细胞抑制人单核细胞THP-1活化及其分子机制[J]. 山东大学学报（医学版）, 2017, 55(3): 49-53.
[5] 吴倩,倪阳,杨清锐,孙红胜. 双眼睑肿胀及双侧颌下包块1例——IgG4相关性疾病的诊断与思考[J]. 山东大学学报（医学版）, 2017, 55(11): 93-96.
[6] 杨延军,梁静,李芳,杜令席. 常山酮对小鼠子宫内膜异位症巨噬细胞极化的调控作用[J]. 山东大学学报（医学版）, 2016, 54(9): 26-31.
[7] 刘蒙蒙,赵翠芬,孔清玉,蔡直锋,夏伟. miR-1/133对病毒性心肌炎小鼠心肌细胞离子通道表达的影响[J]. 山东大学学报（医学版）, 2016, 54(8): 6-11.
[8] 王庆石,陈允震,刘海春,武文亮,焦广俊,李晓峰,徐大霞. 髓核细胞分泌的炎症因子对后纵韧带成纤维细胞增殖与成骨能力的影响[J]. 山东大学学报（医学版）, 2016, 54(6): 22-26.
[9] 李红志,刘静,宋岩,迟令懿,刘玉光. 利拉鲁肽对脊髓损伤修复作用的探讨[J]. 山东大学学报（医学版）, 2016, 54(4): 1-5.
[10] 孙鹏飞,孟晓,张凯,黎莉. 抵抗素样分子 β在动脉粥样硬化斑块稳定性中的作用[J]. 山东大学学报（医学版）, 2016, 54(3): 1-4.
[11] 袁冰,李冉冉,韩明勇. 恶性黑色素瘤调节肺组织微环境并促进肿瘤肺转移的实验研究[J]. 山东大学学报（医学版）, 2016, 54(11): 13-18.
[12] 李少伟, 刘宗正, 刘春霞, 张焱如, 周欢敏. 葡萄籽原花青素对小鼠脂肪肝模型缺血再灌注造成的急性肝损伤的保护作用[J]. 山东大学学报（医学版）, 2015, 53(9): 41-46.
[13] 张荣军, 韩波, 高聆, 朱梅, 丁国玉, 梁燕. siRNA沉默CD40基因对实验性自身免疫性心肌炎大鼠的作用及IL-22表达的影响[J]. 山东大学学报（医学版）, 2015, 53(5): 36-40.
[14] 刘洋, 王焕亮, 刘亚洋, 闫红丹, 孙宝柱, 黄珊珊, 黄瑞, 类维富. 利多卡因对脂多糖诱导小鼠巨噬细胞caspase-1和IL-1β表达的影响[J]. 山东大学学报（医学版）, 2015, 53(12): 43-46,56.
[15] 李少伟, 刘宗正, 刘春霞, 张焱如, 周欢敏. 口服富氢水对小鼠脂肪肝缺血再灌注损伤的保护作用[J]. 山东大学学报（医学版）, 2015, 53(1): 10-15.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2018 《山东大学学报(医学版)》编辑部　
地址:山东大学科技期刊社（济南市历城区山大南路27号山东大学中心校区明德楼B座721室） 电话: 0531-88366918 E-mail: xbyxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发