芜菁挥发油对高脂高糖小鼠降血糖的作用机制

doi:10.6040/j.issn.1671-7554.0.2014.172

摘要/Abstract

摘要： 目的探讨芜菁挥发油成分对高脂高糖小鼠动物模型的降血糖作用机制。方法采用超临界二氧化碳萃取仪提取芜菁挥发油成分。高糖高脂饲料喂养小鼠4周后,连续3 d腹腔注射链脲佐菌素(STZ)100 mg/kg,制备小鼠2型糖尿病模型,血糖测定仪尾静脉采血,测空腹血糖, 小鼠血糖大于11.0 mmol/L即为造模成功。将小鼠随机分为空白对照组、模型组、芜菁挥发油高剂量[40 g/(kg·d)]组、芜菁挥发油低剂量[20 g/(kg·d)]组、阳性药物(盐酸二甲双胍)组,每日灌胃1次,连续14 d,分别于造模前,造模后,用药后3、7及14 d剪尾取血,检测血糖。小鼠摘眼球取血,血清测定糖化血红蛋白(HbA1c)、甘油三酯(TG)、总胆固醇(TC)、血清胰岛素(INS)水平;剖取胸腺、脾脏,计算脏器指数。结果高、低剂量的芜菁挥发油均可降低2型糖尿病模型小鼠的血糖水平(P< 0.01)及HbA1c水平(P<0.05),升高INS水平(P<0.05),显著降低TG及TC水平(P<0.01),保护胸腺,增强机体的免疫能力。结论芜菁挥发油成分对链脲佐菌素联合高脂高糖小鼠糖尿病模型有较明显的降低血糖及血脂的作用,其机制可能是通过干预HbA1c的形成、提高INS水平来降低高脂高糖小鼠血糖。

关键词: 甘油三酯, 芜菁挥发油, 血清胰岛素, 总胆固醇, 糖化血红蛋白

Abstract: Objective To explore the hypoglycemic mechanism of plateau plant Brassica rapa L. (turnip) volatile oil in an animal model of diabetic mice. Methods The volatile oil was extracted using supercritical carbon dioxide extraction method. The diabetic mice model was built by feeding high-fat and high-carbohydrate diet for 4 weeks then intraperitoneal injection of streptozotocin (STZ) 100 mg/kg for 3 days. The mice with fasting blood glucose in tail vein being greater than 11.0 mmol/L were defined as successful experimental models. Fifty mice were randomly divided into control group, model group, high dose of turnip volatile oil group, low dose of turnip volatile oil group, and positive control (metformin hydrochloride) group, 10 in each group. The mice were perfused for 14 days at one time per day. The blood was collected from tail vein and the blood glucose level was measured before and after building the models, and at day 3, 7, 14 after medication. The blood samples were taken from the mice's eyeballs and the glycosylated hemoglobin (HbA1c), triglyceride (TG), total cholesterol (TC), and serum insulin (INS) levels were measured. The fresh spleen and thymus gland were cut and weighed to compute organ index. Results Both high dose and low dose of turnip volatile oil had significant influences on the type 2 diabetic model mice, including reduced blood glucose (P<0.01) and HbA1c levels (P<0.05), increased INS level (P<0.05), and decreased TG and TC levels (P<0.01). The turnip volatile oil could protect the thymus in diabetic mice, and enhance the immune ability. Conclusion Turnip volatile oil can reduce hyperglycemia significantly in diabetic model induced by STZ, which may due to the intervention of the biosynthesis of HbAlc and increase of INS level.

Key words: Serum insulin, Tri-glyceride, Glycosylated hemoglobin, Total cholesterol, Brassica rapa L. (turnip) volatile oil

中图分类号:

R285.5

陈湘宏, 刘燕, 翁裕馨, 康文娟, 杨仕兵. 芜菁挥发油对高脂高糖小鼠降血糖的作用机制[J]. 山东大学学报（医学版）, 2014, 52(12): 20-23.

CHEN Xianghong, LIU Yan, WENG Yuxin, KANG Wenjuan, YANG Shibing. Hypoglycemic mechanism of volatile oil of plateau plant Brassica rapa L. in hyperlipidemic and hyperglycemic mice[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(12): 20-23.

参考文献

[1] 彭彤,梁慧,郭亦然,等.芜菁根挥发油化学成分的气相色谱-质谱联用分析[J].时珍国医国药, 2009, 20(12):3000-3002. PENG Tong, LIANG Hui, GUO Yiran, et al. Analysis on the volatile oils from Brassica rapa L. by GC-MS[J]. LISHIZHEN Medicine and Materia Medica Research, 2009, 20(12): 3000-3002.
[2] 陈湘宏,文绍敦,吴萍,等.芜菁不同提取物对糖尿病模型小鼠降血糖作用的研究[J].中国药房, 2013, 24(7):596-598. CHEN Xianghong, WEN Shaodun, WU Ping. Study on hypoglycemic effect of different extracts of Brassica rapa on diabetic model mice[J]. China Pharmacy, 2013, 24(7):596-598.
[3] 朱爱勤.糖尿病证治八型[J].黑龙江中医药, 2001(4):5-6.
[4] Srinivasan K, Viswanad B, Asrat L, et al. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: a model for type 2 diabetes and pharmacological screening[J]. Pharmacological Research, 2005, 52(4):313-320.
[5] Zhang M, Lv X Y, Li J, et al. The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model[J]. Exp Diabetes Res, 2008, 2008:704045.
[6] 刘文晖.早期糖尿病肾脏微血管病变对糖化血红蛋白及尿微量白蛋白水平的影响[J].临床合理用药, 2011, 4(3B):9-10. LIU Wenhui. Effect of early diabetic renal microangiopathy with glycated hemoglobin (HbAlc) and urinary microproteins[J]. Chin J of Clinical Rational Drug Use, 2011, 4(3B):9-10.
[7] Yamato T, Nakamura T, Nobel N A, et al. Expression of transforming growth factor-β is elevated in human and experimental diabetic nephropathy [J]. Proc Natl Acad Sci U S A, 1993, 90(5):1814-1818.
[8] Yamato T, Nobel N A, Cohen A H, et al. Expression of transforming growth factor-beta isofomns in human glomerular diseases[J]. Kidney Int, 1996, 49(2):461-469.
[9] Shankland S J, Scholey J W, Ly H, et al. Expression oftransforming growth factor-β1 during diabetic renal hypertrophy [J]. Kidney Int, 1994, 46(6):430-432.
[10] 苏丽.糖化血红蛋白与糖尿病及其并发症的相关性探讨[J].临床和实验医学杂志, 2011, 10(10):771-772.
[11] Eckel R H, York D A, Rossner S, et al. Prevention Conference VII: obesity, a worldwide epidemic related to heart disease and stroke: executive summary[J]. Circulation, 2004, 110(18):2968-2975.
[12] Barnett A H. Dyslipidaemia in diabetes—a GP guide[J]. Practitioner, 2002, 246 (1631):120-123.
[13] Smith S R, Ravussin E. Emerging paradigms for understanding fatness and diabetes risk[J]. Curr Diab Rep, 2002, 2(3): 223-230.
[14] Unger R H. Lipotoxic diseases[J]. Annu Rev Med, 2002, 53:319-336.
[15] Aissaoui A, Zizi S, Israili Z H, et al. Hypoglycemic and hypolipidemic effects of Coriandrum sativum L. in Meriones shawi rats[J]. J Ethnopharmacol, 2011, 137(1): 652-661.

相关文章 10

[1]	韩云凤, 徐华, 刘骞, 孙汉辰, 许丹丹, 侯为开, 黄庆先. 血糖水平对糖尿病大鼠心肌GLUT1和GLUT4 mRNA表达的影响[J]. 山东大学学报（医学版）, 2015, 53(5): 27-30.
[2]	靳成伟, 李葵, 赵静, 岳欣, 尚嫄嫄, 韩露, 张运, 张薇, 马骁, 钟明. 外周血单核细胞甘油三酯水解酶的表达与代谢综合征的相关性[J]. 山东大学学报（医学版）, 2014, 52(8): 39-42.
[3]	王川1，侯新国1，梁凯1，闫飞1，杨俊鹏1，王令舒1，田萌1，李成乔2，张秀萍3，杨位芳4，马泽强5，陈丽1. HDL-C和TG/HDL-C比值对山东省中老年回族人胰岛素抵抗的预测价值[J]. 山东大学学报（医学版）, 2014, 52(5): 73-76.
[4]	张楠1，袁中尚2，张栩1，张祎坤1，于春晓1，高聆3，赵家军1，管庆波1，管理英4. 2型糖尿病男性患者血清睾酮与血脂关系的探讨[J]. 山东大学学报（医学版）, 2014, 52(4): 58-61.
[5]	黄芬，蒋钰 . IGT患者高甘油三酯血症-腰围表型与早期认知障碍的关系[J]. 山东大学学报（医学版）, 2014, 52(3): 72-74.
[6]	郭娅棣，高燕燕，李莉，孙瑞霞，王静，咸玉欣. 兰索拉唑对2型糖尿病患者血糖及胃泌素的影响[J]. 山东大学学报(医学版), 2013, 51(7): 62-66.
[7]	王孝勇1，李士雪2，孙秀彬2，逄增昌3，乔青4. 5种方法筛查无症状2型糖尿病的效果评价[J]. 山东大学学报(医学版), 2011, 49(4): 158-162.
[8]	孙林林，石军郝菁华，任万华. 低分子肝素对高脂饮食大鼠非酒精性脂肪性肝炎模型的影响[J]. 山东大学学报(医学版), 2010, 48(11): 58-62.
[9]	尹小芳，侯新国，孙宇，矫磊，刘庆红，马爱霞，范琳琳，张亮，曹立君，陈丽. 2型糖尿病患者糖化血红蛋白达标率及影响因素现状调查[J]. 山东大学学报(医学版), 2010, 48(1): 12-16.
[10]	王莉莉，刘兆兰，房盛夏，贾崇奇 . 乙醇脱氢酶1B基因变异与高甘油三酯血症对早发冠心病的影响[J]. 山东大学学报(医学版), 2009, 47(02): 103-105.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed