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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (5): 21-26.doi: 10.6040/j.issn.1671-7554.0.2014.1002

• 基础医学 • 上一篇    下一篇

长链非编码RNA在压力超负荷引起的大鼠心肌肥厚中的差异表达

姜蕾, 张磊, 梁江久   

  1. 山东大学附属千佛山医院保健心内一科, 山东 济南 250014
  • 收稿日期:2014-12-29 修回日期:2015-04-01 出版日期:2015-05-10 发布日期:2015-05-10
  • 通讯作者: 梁江久。E-mail:liangjiangjiu@163.com E-mail:liangjiangjiu@163.com
  • 基金资助:
    山东省自然科学基金(ZR2010HM116)

Expression profiles of long noncoding RNAs in rat myocardial hypertrophy induced by pressure overload

JIANG Lei, ZHANG Lei, LIANG Jiangjiu   

  1. Department of Cardiovascular Health Care No.1, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2014-12-29 Revised:2015-04-01 Online:2015-05-10 Published:2015-05-10

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摘要： 目的 探讨长链非编码RNA(lncRNA)在腹主动脉缩窄术大鼠心肌肥厚和正常大鼠心肌中的差异表达。方法 构建腹主动脉缩窄大鼠模型,术后4周测量大鼠超声心动图参数、左室质量指数,通过HE染色观察心肌细胞的面积,采用qRT-PCR法检测心肌肥厚相关因子ANF、β-MHC mRNA的表达水平,验证压力超负荷模型构建情况。采用lncRNA芯片技术检测lncRNA表达谱,筛选出具有差异性表达的lncRNA,并通过qRT-PCR验证芯片结果准确性。利用lncRNA与mRNA特异性表达的标准化信号强度,构建lncRNA与靶基因的共表达网络。结果 大鼠心肌肥厚中共检测出6 969条lncRNA,其中显著上调表达80条,显著下调表达172条。经qRT-PCR检测显示,在大鼠心肌肥厚中MRAK134201下调表达,X89963上调表达,与芯片结果一致。构建共表达网络发现,与XR_008680共表达的蛋白编码基因111个。结论 lncRNA在压力超负荷性大鼠心脏组织中的表达谱发生变化,提示lncRNA可能在心肌肥厚的发生发展中具有一定的作用。

关键词: 心肌肥厚, 长链非编码RNA, 大鼠, 长链非编码RNA芯片, 压力超负荷

Abstract: Objective To analyze the expressions of long noncoding RNA (lncRNA) in rat myocardial hypertrophy induced by abdominal aortic banding and in healthy rat hearts. Methods Rat models with myocardial hypertrophy induced by abdominal aortic banding were established. After 4 weeks, the echocardiographic data, left ventricular mass index and the myocyte cross-section were tested. Expressions of ANF and β-MHC mRNA were determined with quantitative real-time polymerase chain reaction (qRT-PCR). Differences of lncRNA expression profiles were inspected with lncRNA microarray and validated with qRT-PCR. Gene co-expression network was built with normalized signal intensity of specifically expressed lncRNAs and mRNAs. Results We identified 6 969 lncRNAs, among which 80 were significantly up-regulated and 172 down-regulated. The expressions of lncRNA MRAK134201 and X89963 verified by qRT-PCR and microarray data were consistent. The network constructed from lncRNA XR_008680 was co-expressed with 111 coding mRNAs. Conclusion Long noncoding RNAs are differentially expressed in rat cardiac hypertrophy models, indicating that lncRNAs might play a role in the pathogenesis of myocardial hypertrophy.

Key words: Pressure overload, LncRNA array, Rats, Myocardial hypertrophy, Long noncoding RNA

中图分类号: 

  • R541
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